FAK抑制剂对肝癌HCC-LM3细胞骨架重排和侵袭的作用机制
目的基于FAK/PI3K/Akt通路，探讨FAK抑制剂CT-707对人肝癌HCC-LM3细胞骨架重排、侵袭、迁移及裸鼠皮下移植瘤生长的影响和机制。方法HCC-LM3细胞分为Control组、CT-707低剂量（1.5 μmol/L）组、CT-707中剂量（3 μmol/L）组、CT-707高剂量（6 μmol/L）组，采用Transwell小室实验、细胞划痕、MTT实验分别测定细胞侵袭、迁移能力及细胞活力；采用RT-qPCR和Western blot分别检测Palladin、Vimentin、MMP2和MMP9 mRNA及蛋白的表达；Western blot检测p-FAK、FAK、p-PI3K...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Sun Yat-sen University
2021-05-01
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| Series: | Zhongshan Daxue xuebao. Yixue kexue ban |
| Subjects: | |
| Online Access: | http://xuebaoyx.sysu.edu.cn/zh/article/doi/10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2021.0106/ |
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