Unraveling the transepithelial absorption of novel dark blue pigment from Vaccinium bracteatum Thunb. leaves on the Caco-2 intestinal cell
The dark blue pigment (DBP) is a health ingredient from Vaccinium bracteatum Thunb. leaves used as a functional food supplement. However, the details of transepithelial absorption on the intestinal epithelial cells are barely understood. This study aimed to clarify the absorption properties of DBP i...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2025-03-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2024.9250062 |
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| author | Mingcong Fan Weijia Lian Wenjun Yao Tingting Li Jiajia Zhao Qiang Li Yanming Guan Yan Li Haifeng Qian Zhiming Rao Li Wang |
| author_facet | Mingcong Fan Weijia Lian Wenjun Yao Tingting Li Jiajia Zhao Qiang Li Yanming Guan Yan Li Haifeng Qian Zhiming Rao Li Wang |
| author_sort | Mingcong Fan |
| collection | DOAJ |
| description | The dark blue pigment (DBP) is a health ingredient from Vaccinium bracteatum Thunb. leaves used as a functional food supplement. However, the details of transepithelial absorption on the intestinal epithelial cells are barely understood. This study aimed to clarify the absorption properties of DBP in the Caco-2 cell monolayer model and evaluate the effect on the endo-metabolism and barrier function of Caco-2 cells. The results showed that the DBP did not show the dose-dependent toxic effect to Caco-2 cells between 0.25 and 1.5 mg/mL, which did not cause disorder in the normal cell metabolism and absorption activity. The Caco-2 cell monolayer model could absorb DBP by passive and active transport, and the absorptive pattern was dose-dependent when the concentration was more than 0.25 mg/mL. During DBP absorption, an increase in mRNA and protein expressions of glucose transporters demonstrated that the glucose transporters were the potential transporter of DBP. But the glucose transport amounts were significantly lowered after 30 min of DBP treatment, indicating that DBP owned the inhibitory effect on glucose transportation. Furthermore, DBP also owned protective effects on the barrier function of intestinal epithelial cells. |
| format | Article |
| id | doaj-art-8a4757de885f4095b47b8fbd4a78f87f |
| institution | DOAJ |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-8a4757de885f4095b47b8fbd4a78f87f2025-08-20T02:41:14ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302025-03-01143925006210.26599/FSHW.2024.9250062Unraveling the transepithelial absorption of novel dark blue pigment from Vaccinium bracteatum Thunb. leaves on the Caco-2 intestinal cellMingcong Fan0Weijia Lian1Wenjun Yao2Tingting Li3Jiajia Zhao4Qiang Li5Yanming Guan6Yan Li7Haifeng Qian8Zhiming Rao9Li Wang10School of Food Science and Technology, State Key Laboratory of Food Science and Technology, National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, ChinaSchool of Food Science and Technology, State Key Laboratory of Food Science and Technology, National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, ChinaSchool of Food Science and Technology, State Key Laboratory of Food Science and Technology, National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, ChinaDepartment of Food Science and Engineering, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, ChinaCollege of Cooking Science and Technology, Jiangsu College of Tourism, Yangzhou 225000, ChinaChina National Institute of Standardization, Beijing 100089, ChinaChina National Research Institute of Food and Fermentation Industries Co., Ltd., Beijing 100015, ChinaSchool of Food Science and Technology, State Key Laboratory of Food Science and Technology, National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, ChinaSchool of Food Science and Technology, State Key Laboratory of Food Science and Technology, National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, ChinaChina National Research Institute of Food and Fermentation Industries Co., Ltd., Beijing 100015, ChinaSchool of Food Science and Technology, State Key Laboratory of Food Science and Technology, National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, ChinaThe dark blue pigment (DBP) is a health ingredient from Vaccinium bracteatum Thunb. leaves used as a functional food supplement. However, the details of transepithelial absorption on the intestinal epithelial cells are barely understood. This study aimed to clarify the absorption properties of DBP in the Caco-2 cell monolayer model and evaluate the effect on the endo-metabolism and barrier function of Caco-2 cells. The results showed that the DBP did not show the dose-dependent toxic effect to Caco-2 cells between 0.25 and 1.5 mg/mL, which did not cause disorder in the normal cell metabolism and absorption activity. The Caco-2 cell monolayer model could absorb DBP by passive and active transport, and the absorptive pattern was dose-dependent when the concentration was more than 0.25 mg/mL. During DBP absorption, an increase in mRNA and protein expressions of glucose transporters demonstrated that the glucose transporters were the potential transporter of DBP. But the glucose transport amounts were significantly lowered after 30 min of DBP treatment, indicating that DBP owned the inhibitory effect on glucose transportation. Furthermore, DBP also owned protective effects on the barrier function of intestinal epithelial cells.https://www.sciopen.com/article/10.26599/FSHW.2024.9250062vaccinium bracteatum thunb. leafdark blue pigmentintestinal epithelial celltransepithelial absorptionepithelial cell barrier |
| spellingShingle | Mingcong Fan Weijia Lian Wenjun Yao Tingting Li Jiajia Zhao Qiang Li Yanming Guan Yan Li Haifeng Qian Zhiming Rao Li Wang Unraveling the transepithelial absorption of novel dark blue pigment from Vaccinium bracteatum Thunb. leaves on the Caco-2 intestinal cell Food Science and Human Wellness vaccinium bracteatum thunb. leaf dark blue pigment intestinal epithelial cell transepithelial absorption epithelial cell barrier |
| title | Unraveling the transepithelial absorption of novel dark blue pigment from Vaccinium bracteatum Thunb. leaves on the Caco-2 intestinal cell |
| title_full | Unraveling the transepithelial absorption of novel dark blue pigment from Vaccinium bracteatum Thunb. leaves on the Caco-2 intestinal cell |
| title_fullStr | Unraveling the transepithelial absorption of novel dark blue pigment from Vaccinium bracteatum Thunb. leaves on the Caco-2 intestinal cell |
| title_full_unstemmed | Unraveling the transepithelial absorption of novel dark blue pigment from Vaccinium bracteatum Thunb. leaves on the Caco-2 intestinal cell |
| title_short | Unraveling the transepithelial absorption of novel dark blue pigment from Vaccinium bracteatum Thunb. leaves on the Caco-2 intestinal cell |
| title_sort | unraveling the transepithelial absorption of novel dark blue pigment from vaccinium bracteatum thunb leaves on the caco 2 intestinal cell |
| topic | vaccinium bracteatum thunb. leaf dark blue pigment intestinal epithelial cell transepithelial absorption epithelial cell barrier |
| url | https://www.sciopen.com/article/10.26599/FSHW.2024.9250062 |
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