REPROGRAMMING OF SPLENIC MONONUCLEAR PHAGOCYTE PHENOTYPE DURING MODELING OF SYSTEMIC JUVENILE IDIOPATHIC ARTHRITISIN WISTAR RATS

Purpose. This study is devoted to the evaluation of the number of splenic alternatively activated (M2, CD163+) macrophages and other mononuclear phagocytes after arthritis development in a model of systemic juvenile arthritis in Wistar rats (with and without modification by doxycycline or dexamethaso...

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Format: Article
Language:Russian
Published: Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University) 2017-09-01
Series:Сеченовский вестник
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Online Access:https://www.sechenovmedj.com/jour/article/view/24
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description Purpose. This study is devoted to the evaluation of the number of splenic alternatively activated (M2, CD163+) macrophages and other mononuclear phagocytes after arthritis development in a model of systemic juvenile arthritis in Wistar rats (with and without modification by doxycycline or dexamethasone). Methods. Cell phenotypes were assessed by flow cytometric analysis. Percentages of classically-activated phagocytes were estimated (M1, CD163-). Results. The indexes of dominating phenotypes are assessed for spleen (no significant differences in animal groups). In comparison we demonstrate indexes for the blood mononuclear phagocytes (significant changes were observed). Nevetherless, we have demonstrated phenotypical changes of splenic phagocyte population at whole. Conclusions. Redistribution of the tissue alternatively-activated cells of phagocyte population in spleen was found to be significantly different in animal groups with doxycycline and dexamethasone administration.
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institution Kabale University
issn 2218-7332
2658-3348
language Russian
publishDate 2017-09-01
publisher Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
record_format Article
series Сеченовский вестник
spelling doaj-art-8a46faceaeb846c4b943a47e9400da122025-08-20T03:59:04ZrusFederal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)Сеченовский вестник2218-73322658-33482017-09-01034923REPROGRAMMING OF SPLENIC MONONUCLEAR PHAGOCYTE PHENOTYPE DURING MODELING OF SYSTEMIC JUVENILE IDIOPATHIC ARTHRITISIN WISTAR RATS0ФГАОУ ВО Первый МГМУ им. И.М. Сеченова Минздрава России (Сеченовский Университет)Purpose. This study is devoted to the evaluation of the number of splenic alternatively activated (M2, CD163+) macrophages and other mononuclear phagocytes after arthritis development in a model of systemic juvenile arthritis in Wistar rats (with and without modification by doxycycline or dexamethasone). Methods. Cell phenotypes were assessed by flow cytometric analysis. Percentages of classically-activated phagocytes were estimated (M1, CD163-). Results. The indexes of dominating phenotypes are assessed for spleen (no significant differences in animal groups). In comparison we demonstrate indexes for the blood mononuclear phagocytes (significant changes were observed). Nevetherless, we have demonstrated phenotypical changes of splenic phagocyte population at whole. Conclusions. Redistribution of the tissue alternatively-activated cells of phagocyte population in spleen was found to be significantly different in animal groups with doxycycline and dexamethasone administration.https://www.sechenovmedj.com/jour/article/view/24the systemic form of juvenile idiopathic arthritismononuclear phagocytesmonocytesmacrophagesphenotype
spellingShingle REPROGRAMMING OF SPLENIC MONONUCLEAR PHAGOCYTE PHENOTYPE DURING MODELING OF SYSTEMIC JUVENILE IDIOPATHIC ARTHRITISIN WISTAR RATS
Сеченовский вестник
the systemic form of juvenile idiopathic arthritis
mononuclear phagocytes
monocytes
macrophages
phenotype
title REPROGRAMMING OF SPLENIC MONONUCLEAR PHAGOCYTE PHENOTYPE DURING MODELING OF SYSTEMIC JUVENILE IDIOPATHIC ARTHRITISIN WISTAR RATS
title_full REPROGRAMMING OF SPLENIC MONONUCLEAR PHAGOCYTE PHENOTYPE DURING MODELING OF SYSTEMIC JUVENILE IDIOPATHIC ARTHRITISIN WISTAR RATS
title_fullStr REPROGRAMMING OF SPLENIC MONONUCLEAR PHAGOCYTE PHENOTYPE DURING MODELING OF SYSTEMIC JUVENILE IDIOPATHIC ARTHRITISIN WISTAR RATS
title_full_unstemmed REPROGRAMMING OF SPLENIC MONONUCLEAR PHAGOCYTE PHENOTYPE DURING MODELING OF SYSTEMIC JUVENILE IDIOPATHIC ARTHRITISIN WISTAR RATS
title_short REPROGRAMMING OF SPLENIC MONONUCLEAR PHAGOCYTE PHENOTYPE DURING MODELING OF SYSTEMIC JUVENILE IDIOPATHIC ARTHRITISIN WISTAR RATS
title_sort reprogramming of splenic mononuclear phagocyte phenotype during modeling of systemic juvenile idiopathic arthritisin wistar rats
topic the systemic form of juvenile idiopathic arthritis
mononuclear phagocytes
monocytes
macrophages
phenotype
url https://www.sechenovmedj.com/jour/article/view/24