Measurement of Anti-TNF Biologics in Serum Samples of Pediatric Patients: Comparison of Enzyme-Linked Immunosorbent Assay (ELISA) with a Rapid and Automated Fluorescence-Based Lateral Flow Immunoassay

Measurement of Anti-TNF Biologics in Serum Samples of Pediatric Patients: Comparison of Enzyme-Linked Immunosorbent Assay (ELISA) with a Rapid and Automated Fluorescence-Based Lateral Flow Immunoassay

<b>Background</b>: Therapeutic drug monitoring (TDM) of infliximab (IFX) and adalimumab (ADL) mainly relies on the use of enzyme-linked immunosorbent assays (ELISA). More recently, rapid assays have been developed and validated to reduce turnaround time (TAT). Here, we compared IFX and A...

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Main Authors: Chiara Rossi, Raffaele Simeoli, Giulia Angelino, Sara Cairoli, Fiammetta Bracci, Daniela Knafelz, Erminia Francesca Romeo, Simona Faraci, Giusyda Tarantino, Alessandro Mancini, Alessia Vitale, Carlo Dionisi Vici, Silvia Magni Manzoni, Paola De Angelis, Bianca Maria Goffredo
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Pharmaceutics
Subjects:
pediatric patients
therapeutic drug monitoring (TDM)
anti-TNF biological agents
enzyme-linked immunosorbent assay (ELISA)
fluorescence-based lateral flow immunoassay (AFIAS)
turnaround time (TAT)
Online Access:https://www.mdpi.com/1999-4923/17/4/421
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