Unraveling gene expression: a beginner’s guide from chromatin modifications to mRNA export in Saccharomyces cerevisiae
Understanding gene expression requires grasping its multi-step processes, from chromatin remodeling to mRNA export. This manuscript provides an accessible entry point for PhD students and junior postdocs beginning research in this area, using yeast as a model organism. We present a beginner-friendly...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Nucleus |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19491034.2025.2516909 |
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| author | Joan Serrano-Quílez Susana Rodriguez-Navarro |
| author_facet | Joan Serrano-Quílez Susana Rodriguez-Navarro |
| author_sort | Joan Serrano-Quílez |
| collection | DOAJ |
| description | Understanding gene expression requires grasping its multi-step processes, from chromatin remodeling to mRNA export. This manuscript provides an accessible entry point for PhD students and junior postdocs beginning research in this area, using yeast as a model organism. We present a beginner-friendly overview of gene expression, emphasizing the dynamic interplay between chromatin modifications, transcription, mRNA processing, and export. Key topics include chromatin organization, with a focus on H2B ubiquitylation and H3 methylation crosstalk; transcriptional control by RNA polymerase II, including initiation, elongation, and termination; and the export of mRNAs via Mex67–Mtr2, adaptor proteins, and the TREX and TREX-2 complexes at the nuclear pore complex. Relevant examples from yeast genetics, biochemistry, and structural biology illustrate each step. This overview aims to equip new researchers with foundational knowledge and provides references to key studies, current challenges, and open questions in the regulation of gene expression. |
| format | Article |
| id | doaj-art-8a3a41ed373744e38443e1f4553a174e |
| institution | OA Journals |
| issn | 1949-1034 1949-1042 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Nucleus |
| spelling | doaj-art-8a3a41ed373744e38443e1f4553a174e2025-08-20T02:33:39ZengTaylor & Francis GroupNucleus1949-10341949-10422025-12-0116110.1080/19491034.2025.2516909Unraveling gene expression: a beginner’s guide from chromatin modifications to mRNA export in Saccharomyces cerevisiaeJoan Serrano-Quílez0Susana Rodriguez-Navarro1Instituto de Biomedicina de Valencia (IBV), CSIC, Valencia, SpainInstituto de Biomedicina de Valencia (IBV), CSIC, Valencia, SpainUnderstanding gene expression requires grasping its multi-step processes, from chromatin remodeling to mRNA export. This manuscript provides an accessible entry point for PhD students and junior postdocs beginning research in this area, using yeast as a model organism. We present a beginner-friendly overview of gene expression, emphasizing the dynamic interplay between chromatin modifications, transcription, mRNA processing, and export. Key topics include chromatin organization, with a focus on H2B ubiquitylation and H3 methylation crosstalk; transcriptional control by RNA polymerase II, including initiation, elongation, and termination; and the export of mRNAs via Mex67–Mtr2, adaptor proteins, and the TREX and TREX-2 complexes at the nuclear pore complex. Relevant examples from yeast genetics, biochemistry, and structural biology illustrate each step. This overview aims to equip new researchers with foundational knowledge and provides references to key studies, current challenges, and open questions in the regulation of gene expression.https://www.tandfonline.com/doi/10.1080/19491034.2025.2516909ChromatinepigeneticsmRNA exporttranscriptionyeast |
| spellingShingle | Joan Serrano-Quílez Susana Rodriguez-Navarro Unraveling gene expression: a beginner’s guide from chromatin modifications to mRNA export in Saccharomyces cerevisiae Nucleus Chromatin epigenetics mRNA export transcription yeast |
| title | Unraveling gene expression: a beginner’s guide from chromatin modifications to mRNA export in Saccharomyces cerevisiae |
| title_full | Unraveling gene expression: a beginner’s guide from chromatin modifications to mRNA export in Saccharomyces cerevisiae |
| title_fullStr | Unraveling gene expression: a beginner’s guide from chromatin modifications to mRNA export in Saccharomyces cerevisiae |
| title_full_unstemmed | Unraveling gene expression: a beginner’s guide from chromatin modifications to mRNA export in Saccharomyces cerevisiae |
| title_short | Unraveling gene expression: a beginner’s guide from chromatin modifications to mRNA export in Saccharomyces cerevisiae |
| title_sort | unraveling gene expression a beginner s guide from chromatin modifications to mrna export in saccharomyces cerevisiae |
| topic | Chromatin epigenetics mRNA export transcription yeast |
| url | https://www.tandfonline.com/doi/10.1080/19491034.2025.2516909 |
| work_keys_str_mv | AT joanserranoquilez unravelinggeneexpressionabeginnersguidefromchromatinmodificationstomrnaexportinsaccharomycescerevisiae AT susanarodrigueznavarro unravelinggeneexpressionabeginnersguidefromchromatinmodificationstomrnaexportinsaccharomycescerevisiae |