From Spectra to Signatures: Detecting Fentanyl in Human Nails with ATR–FTIR and Machine Learning
Human nails have recently become a sample of interest for toxicological purposes. Multiple studies have proven the ability to detect various analytes within the keratin matrix of the nail. The analyte of interest in this study is fentanyl, a highly dangerous and abused drug in recent decades. In thi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-01-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/25/1/227 |
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| author | Aubrey Barney Václav Trojan Radovan Hrib Ashley Newland Jan Halámek Lenka Halámková |
| author_facet | Aubrey Barney Václav Trojan Radovan Hrib Ashley Newland Jan Halámek Lenka Halámková |
| author_sort | Aubrey Barney |
| collection | DOAJ |
| description | Human nails have recently become a sample of interest for toxicological purposes. Multiple studies have proven the ability to detect various analytes within the keratin matrix of the nail. The analyte of interest in this study is fentanyl, a highly dangerous and abused drug in recent decades. In this proof-of-concept study, ATR–FTIR was combined with machine learning methods, which are effective in detecting and differentiating fentanyl in samples, to explore whether nail samples are distinguishable from individuals who have used fentanyl and those who have not. PLS-DA and SVM-DA prediction models were created for this study and had an overall accuracy rate of 84.8% and 81.4%, respectively. Notably, when classification was considered at the donor level—i.e., determining whether the donor of the nail sample was using fentanyl—all donors were correctly classified. These results show that ATR–FTIR spectroscopy in combination with machine learning can effectively differentiate donors who have used fentanyl and those who have not and that human nails are a viable sample matrix for toxicology. |
| format | Article |
| id | doaj-art-8a30a63b68564d02bb4f0d8a6ae57000 |
| institution | OA Journals |
| issn | 1424-8220 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Sensors |
| spelling | doaj-art-8a30a63b68564d02bb4f0d8a6ae570002025-08-20T02:36:03ZengMDPI AGSensors1424-82202025-01-0125122710.3390/s25010227From Spectra to Signatures: Detecting Fentanyl in Human Nails with ATR–FTIR and Machine LearningAubrey Barney0Václav Trojan1Radovan Hrib2Ashley Newland3Jan Halámek4Lenka Halámková5Department of Environmental Toxicology, Texas Tech University, Lubbock, TX 79409, USACannabis Facility, International Clinical Research Centre, St. Anne’s University Hospital Brno, 60200 Brno, Czech RepublicCannabis Facility, International Clinical Research Centre, St. Anne’s University Hospital Brno, 60200 Brno, Czech RepublicDepartment of Environmental Toxicology, Texas Tech University, Lubbock, TX 79409, USADepartment of Environmental Toxicology, Texas Tech University, Lubbock, TX 79409, USADepartment of Environmental Toxicology, Texas Tech University, Lubbock, TX 79409, USAHuman nails have recently become a sample of interest for toxicological purposes. Multiple studies have proven the ability to detect various analytes within the keratin matrix of the nail. The analyte of interest in this study is fentanyl, a highly dangerous and abused drug in recent decades. In this proof-of-concept study, ATR–FTIR was combined with machine learning methods, which are effective in detecting and differentiating fentanyl in samples, to explore whether nail samples are distinguishable from individuals who have used fentanyl and those who have not. PLS-DA and SVM-DA prediction models were created for this study and had an overall accuracy rate of 84.8% and 81.4%, respectively. Notably, when classification was considered at the donor level—i.e., determining whether the donor of the nail sample was using fentanyl—all donors were correctly classified. These results show that ATR–FTIR spectroscopy in combination with machine learning can effectively differentiate donors who have used fentanyl and those who have not and that human nails are a viable sample matrix for toxicology.https://www.mdpi.com/1424-8220/25/1/227fentanylfingernailstoenailsATR–FTIRmachine learningPLS-DA |
| spellingShingle | Aubrey Barney Václav Trojan Radovan Hrib Ashley Newland Jan Halámek Lenka Halámková From Spectra to Signatures: Detecting Fentanyl in Human Nails with ATR–FTIR and Machine Learning Sensors fentanyl fingernails toenails ATR–FTIR machine learning PLS-DA |
| title | From Spectra to Signatures: Detecting Fentanyl in Human Nails with ATR–FTIR and Machine Learning |
| title_full | From Spectra to Signatures: Detecting Fentanyl in Human Nails with ATR–FTIR and Machine Learning |
| title_fullStr | From Spectra to Signatures: Detecting Fentanyl in Human Nails with ATR–FTIR and Machine Learning |
| title_full_unstemmed | From Spectra to Signatures: Detecting Fentanyl in Human Nails with ATR–FTIR and Machine Learning |
| title_short | From Spectra to Signatures: Detecting Fentanyl in Human Nails with ATR–FTIR and Machine Learning |
| title_sort | from spectra to signatures detecting fentanyl in human nails with atr ftir and machine learning |
| topic | fentanyl fingernails toenails ATR–FTIR machine learning PLS-DA |
| url | https://www.mdpi.com/1424-8220/25/1/227 |
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