Risk of residual/recurrent cervical diseases in HPV-positive women post-conization depends on HPV integration status
Abstract Background It is crucial to identify post-operative patients with HPV infection who are at high risk for residual/recurrent disease. This study aimed to evaluate the association between HPV integration and clinical outcomes in HPV-positive women after cervical conization, as well as to iden...
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BMC
2025-01-01
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| Series: | Infectious Agents and Cancer |
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| Online Access: | https://doi.org/10.1186/s13027-025-00637-3 |
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| author | Wenyu Lin Yuxuan Huang Yan Zhang Lixiang Huang Hongning Cai Guanxiang Huang Ye Li Qiaoyu Zhang Huifeng Xue Binhua Dong Pengming Sun |
| author_facet | Wenyu Lin Yuxuan Huang Yan Zhang Lixiang Huang Hongning Cai Guanxiang Huang Ye Li Qiaoyu Zhang Huifeng Xue Binhua Dong Pengming Sun |
| author_sort | Wenyu Lin |
| collection | DOAJ |
| description | Abstract Background It is crucial to identify post-operative patients with HPV infection who are at high risk for residual/recurrent disease. This study aimed to evaluate the association between HPV integration and clinical outcomes in HPV-positive women after cervical conization, as well as to identify HPV integration breakpoints. Methods This retrospective study analyzed data of 791 women who underwent cervical conization for cervical intraepithelial neoplasia grades 2–3 (CIN2-3) between September 2019 and September 2023, sourced from the Fujian and Hubei cervical lesion screening cohorts. Among these, 73 women with HPV infection post-conization underwent HPV integration test within 3 months after a positive HPV test. HPV integration test was performed using the high-throughput viral integration detection (HIVID), a sensitive method for genome-wide survey of HPV integration breakpoints. Results Among the 73 participants with HPV infection post-conization, 10 cases (13.7%) were positive for HPV integration. The logistic regression analysis showed a higher residual/recurrent lesions risk in patients with HPV integration (OR = 3.917, p = 0.048). According to the Kaplan-Meier analysis, age ≥ 45 years (p = 0.016) and HPV integration (p = 0.035) were associated with a higher risk of residual/recurrent CIN at the 1-year follow-up. HPV 52 accounted for the majority of HPV integration genotype (3/10, 30.0%). Surprisingly, HPV 16 had the highest number of HPV average integration sequencing reads (n = 129), followed by HPV 31, 58, 52, 59, 35, and 39. The study also identified 13 HPV breakpoints, including TP63, TLR4, USP10, etc. Conclusions HPV integration was identified as an independent risk factor for residual/recurrent CIN in HPV-positive women post-conization. Women with positive HPV integration should pay attention to careful post-treatment follow-up. |
| format | Article |
| id | doaj-art-8a2e5e068d684812800eb7e807a8fc5d |
| institution | Kabale University |
| issn | 1750-9378 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
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| series | Infectious Agents and Cancer |
| spelling | doaj-art-8a2e5e068d684812800eb7e807a8fc5d2025-02-02T12:09:31ZengBMCInfectious Agents and Cancer1750-93782025-01-0120111110.1186/s13027-025-00637-3Risk of residual/recurrent cervical diseases in HPV-positive women post-conization depends on HPV integration statusWenyu Lin0Yuxuan Huang1Yan Zhang2Lixiang Huang3Hongning Cai4Guanxiang Huang5Ye Li6Qiaoyu Zhang7Huifeng Xue8Binhua Dong9Pengming Sun10College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical UniversityCollege of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical UniversityThe State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Public Health, Xiamen UniversityCollege of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical UniversityDepartment of Gynecology, Maternal and Child Health Hospital of Hubei Province (Women and Children’s Hospital of Hubei Province)College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical UniversityDepartment of Obstetrics and Gynecology, The Third Affiliated Hospital of Zhengzhou UniversityDepartment of Gynecology, Military Hospital of the 73rd Army GroupFujian Provincial Cervical Disease Diagnosis and Treatment Health Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical UniversityCollege of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical UniversityCollege of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical UniversityAbstract Background It is crucial to identify post-operative patients with HPV infection who are at high risk for residual/recurrent disease. This study aimed to evaluate the association between HPV integration and clinical outcomes in HPV-positive women after cervical conization, as well as to identify HPV integration breakpoints. Methods This retrospective study analyzed data of 791 women who underwent cervical conization for cervical intraepithelial neoplasia grades 2–3 (CIN2-3) between September 2019 and September 2023, sourced from the Fujian and Hubei cervical lesion screening cohorts. Among these, 73 women with HPV infection post-conization underwent HPV integration test within 3 months after a positive HPV test. HPV integration test was performed using the high-throughput viral integration detection (HIVID), a sensitive method for genome-wide survey of HPV integration breakpoints. Results Among the 73 participants with HPV infection post-conization, 10 cases (13.7%) were positive for HPV integration. The logistic regression analysis showed a higher residual/recurrent lesions risk in patients with HPV integration (OR = 3.917, p = 0.048). According to the Kaplan-Meier analysis, age ≥ 45 years (p = 0.016) and HPV integration (p = 0.035) were associated with a higher risk of residual/recurrent CIN at the 1-year follow-up. HPV 52 accounted for the majority of HPV integration genotype (3/10, 30.0%). Surprisingly, HPV 16 had the highest number of HPV average integration sequencing reads (n = 129), followed by HPV 31, 58, 52, 59, 35, and 39. The study also identified 13 HPV breakpoints, including TP63, TLR4, USP10, etc. Conclusions HPV integration was identified as an independent risk factor for residual/recurrent CIN in HPV-positive women post-conization. Women with positive HPV integration should pay attention to careful post-treatment follow-up.https://doi.org/10.1186/s13027-025-00637-3HPV integrationCervical intraepithelial neoplasiaPost-treatment surveillance |
| spellingShingle | Wenyu Lin Yuxuan Huang Yan Zhang Lixiang Huang Hongning Cai Guanxiang Huang Ye Li Qiaoyu Zhang Huifeng Xue Binhua Dong Pengming Sun Risk of residual/recurrent cervical diseases in HPV-positive women post-conization depends on HPV integration status Infectious Agents and Cancer HPV integration Cervical intraepithelial neoplasia Post-treatment surveillance |
| title | Risk of residual/recurrent cervical diseases in HPV-positive women post-conization depends on HPV integration status |
| title_full | Risk of residual/recurrent cervical diseases in HPV-positive women post-conization depends on HPV integration status |
| title_fullStr | Risk of residual/recurrent cervical diseases in HPV-positive women post-conization depends on HPV integration status |
| title_full_unstemmed | Risk of residual/recurrent cervical diseases in HPV-positive women post-conization depends on HPV integration status |
| title_short | Risk of residual/recurrent cervical diseases in HPV-positive women post-conization depends on HPV integration status |
| title_sort | risk of residual recurrent cervical diseases in hpv positive women post conization depends on hpv integration status |
| topic | HPV integration Cervical intraepithelial neoplasia Post-treatment surveillance |
| url | https://doi.org/10.1186/s13027-025-00637-3 |
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