Different immunization methods lead to altered gut flora and varied responses to Mycobacterium tuberculosis infection in mice
Introduction: Vaccination is an essential means for prevention of tuberculosis infection, but the effects of various vaccines on the intestinal flora of mice and their response to Mycobacterium tuberculosis (Mtb) infection remain poorly understood. Methodology: In this study, two different vacci...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Journal of Infection in Developing Countries
2020-10-01
|
| Series: | Journal of Infection in Developing Countries |
| Subjects: | |
| Online Access: | https://jidc.org/index.php/journal/article/view/12697 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849324160081199104 |
|---|---|
| author | Jianguo Guo Jun Tang Taisheng Kang Yi Xiong Zhiguang Xiang Chuan Qin |
| author_facet | Jianguo Guo Jun Tang Taisheng Kang Yi Xiong Zhiguang Xiang Chuan Qin |
| author_sort | Jianguo Guo |
| collection | DOAJ |
| description |
Introduction: Vaccination is an essential means for prevention of tuberculosis infection, but the effects of various vaccines on the intestinal flora of mice and their response to Mycobacterium tuberculosis (Mtb) infection remain poorly understood.
Methodology: In this study, two different vaccinations − ESAT6 and ESAT6 + TLR8 agonists - were administered to mice transgenic for human TLR8 to investigate gut microbiota characteristics following vaccination. Gut microbiota was investigated by next generation sequencing in the MiSeq Sequencing System. Adonis analysis was used to evaluate the effect of variables on gut bacterial community stucture. Chao1, Shannon index, and phylogenetic diversity index were used to explore the gut bacterial diversity.
Results: The results showed that different vaccines have significant influence on mice intestinal bacteria (adonis analysis, p < 0.01), with gut bacterial diversity within the ESAT6 + TLR8 agonists group being significantly decreased compared to the ESAT6 treatment group (p < 0.01). Following infection with Mtb via tail vein injection, the bacterial community structure within the control versus vaccinated groups altered significantly (adonis analysis, p < 0.01), and the altered changed genera were markedly different between the groups. Following infection, Bifidobacteria differed between the groups, indicated that they play a vital role in the response to infection.
Conclusions: Our results indicated that different vaccines might have distinct influences on intestinal flora, and their role should not be ignored.
|
| format | Article |
| id | doaj-art-8a2adaeeb88a493f99ba53ac684a7b2c |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2020-10-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-8a2adaeeb88a493f99ba53ac684a7b2c2025-08-20T03:48:47ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802020-10-01141010.3855/jidc.12697Different immunization methods lead to altered gut flora and varied responses to Mycobacterium tuberculosis infection in miceJianguo Guo0Jun Tang1Taisheng Kang2Yi Xiong3Zhiguang Xiang4Chuan Qin5NHC Key Laboratory of Human Disease Comparative Medicine (The Institute of Laboratory Animal Sciences, CAMS and PUMC), Beijing, ChinaNHC Key Laboratory of Human Disease Comparative Medicine (The Institute of Laboratory Animal Sciences, CAMS and PUMC), Beijing, ChinaNHC Key Laboratory of Human Disease Comparative Medicine (The Institute of Laboratory Animal Sciences, CAMS and PUMC), Beijing, ChinaDepartment of Food Science and Engineering, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, ChinaNHC Key Laboratory of Human Disease Comparative Medicine (The Institute of Laboratory Animal Sciences, CAMS and PUMC), Beijing, ChinaNHC Key Laboratory of Human Disease Comparative Medicine (The Institute of Laboratory Animal Sciences, CAMS and PUMC), Beijing, China Introduction: Vaccination is an essential means for prevention of tuberculosis infection, but the effects of various vaccines on the intestinal flora of mice and their response to Mycobacterium tuberculosis (Mtb) infection remain poorly understood. Methodology: In this study, two different vaccinations − ESAT6 and ESAT6 + TLR8 agonists - were administered to mice transgenic for human TLR8 to investigate gut microbiota characteristics following vaccination. Gut microbiota was investigated by next generation sequencing in the MiSeq Sequencing System. Adonis analysis was used to evaluate the effect of variables on gut bacterial community stucture. Chao1, Shannon index, and phylogenetic diversity index were used to explore the gut bacterial diversity. Results: The results showed that different vaccines have significant influence on mice intestinal bacteria (adonis analysis, p < 0.01), with gut bacterial diversity within the ESAT6 + TLR8 agonists group being significantly decreased compared to the ESAT6 treatment group (p < 0.01). Following infection with Mtb via tail vein injection, the bacterial community structure within the control versus vaccinated groups altered significantly (adonis analysis, p < 0.01), and the altered changed genera were markedly different between the groups. Following infection, Bifidobacteria differed between the groups, indicated that they play a vital role in the response to infection. Conclusions: Our results indicated that different vaccines might have distinct influences on intestinal flora, and their role should not be ignored. https://jidc.org/index.php/journal/article/view/12697Gut microbiotavaccineTLR8Mycobacterium tuberculosis |
| spellingShingle | Jianguo Guo Jun Tang Taisheng Kang Yi Xiong Zhiguang Xiang Chuan Qin Different immunization methods lead to altered gut flora and varied responses to Mycobacterium tuberculosis infection in mice Journal of Infection in Developing Countries Gut microbiota vaccine TLR8 Mycobacterium tuberculosis |
| title | Different immunization methods lead to altered gut flora and varied responses to Mycobacterium tuberculosis infection in mice |
| title_full | Different immunization methods lead to altered gut flora and varied responses to Mycobacterium tuberculosis infection in mice |
| title_fullStr | Different immunization methods lead to altered gut flora and varied responses to Mycobacterium tuberculosis infection in mice |
| title_full_unstemmed | Different immunization methods lead to altered gut flora and varied responses to Mycobacterium tuberculosis infection in mice |
| title_short | Different immunization methods lead to altered gut flora and varied responses to Mycobacterium tuberculosis infection in mice |
| title_sort | different immunization methods lead to altered gut flora and varied responses to mycobacterium tuberculosis infection in mice |
| topic | Gut microbiota vaccine TLR8 Mycobacterium tuberculosis |
| url | https://jidc.org/index.php/journal/article/view/12697 |
| work_keys_str_mv | AT jianguoguo differentimmunizationmethodsleadtoalteredgutfloraandvariedresponsestomycobacteriumtuberculosisinfectioninmice AT juntang differentimmunizationmethodsleadtoalteredgutfloraandvariedresponsestomycobacteriumtuberculosisinfectioninmice AT taishengkang differentimmunizationmethodsleadtoalteredgutfloraandvariedresponsestomycobacteriumtuberculosisinfectioninmice AT yixiong differentimmunizationmethodsleadtoalteredgutfloraandvariedresponsestomycobacteriumtuberculosisinfectioninmice AT zhiguangxiang differentimmunizationmethodsleadtoalteredgutfloraandvariedresponsestomycobacteriumtuberculosisinfectioninmice AT chuanqin differentimmunizationmethodsleadtoalteredgutfloraandvariedresponsestomycobacteriumtuberculosisinfectioninmice |