Preferred M2 Polarization by ASC-Based Hydrogel Accelerated Angiogenesis and Myogenesis in Volumetric Muscle Loss Rats
Volumetric muscle loss (VML) injury resulted from massive muscle defects and diseases for which there are still no effective therapeutic treatments. This study aimed to investigate the effects of rat adipose-derived mesenchymal stem cells (rASCs) and rASCs-conditioned medium- (CM-) based type I coll...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/2896874 |
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| author | Hong Huang Jiejie Liu Haojie Hao Deyun Chen Ling Zhizhong Meirong Li Haijing Song Rong Xiang Chaoguang Jiang Xiaobing Fu Weidong Han |
| author_facet | Hong Huang Jiejie Liu Haojie Hao Deyun Chen Ling Zhizhong Meirong Li Haijing Song Rong Xiang Chaoguang Jiang Xiaobing Fu Weidong Han |
| author_sort | Hong Huang |
| collection | DOAJ |
| description | Volumetric muscle loss (VML) injury resulted from massive muscle defects and diseases for which there are still no effective therapeutic treatments. This study aimed to investigate the effects of rat adipose-derived mesenchymal stem cells (rASCs) and rASCs-conditioned medium- (CM-) based type I collagen hydrogel on macrophage (MP) transition, myogenesis, and vascularization in the rat VML model. Laser Doppler results demonstrated much higher blood flow in the rASC- and CM-based hydrogel groups. qRT-PCR, hematoxylin and eosin, immunofluorescence, and Sirius Red staining manifested that both rASCs and CM-based hydrogel implantation accelerated muscle repair with upregulated angiogenesis and myogenesis, attenuated inflammation while facilitating M2 transition, and decreased the collagen deposition compared with the hydrogel group. In vitro experiments indicated that factors secreted from polarized M2 MPs could accelerate the migration and tube formation capacities of HUVECs. These results suggested that rASCs exerted immunomodulatory effects on MPs which further enhanced the proangiogenic potential on ECs to promote myogenesis and angiogenesis during muscle repair. These fundamental results support further clinical applications of ASCs for muscle loss injury. |
| format | Article |
| id | doaj-art-8a210c7fb9bc4b299034e9781d5e2a8f |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-8a210c7fb9bc4b299034e9781d5e2a8f2025-08-20T03:37:50ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/28968742896874Preferred M2 Polarization by ASC-Based Hydrogel Accelerated Angiogenesis and Myogenesis in Volumetric Muscle Loss RatsHong Huang0Jiejie Liu1Haojie Hao2Deyun Chen3Ling Zhizhong4Meirong Li5Haijing Song6Rong Xiang7Chaoguang Jiang8Xiaobing Fu9Weidong Han10Medical School of Nankai University, Tianjin 300071, ChinaMolecular & Immunological Department, Bio-therapeutic Department, Chinese PLA General Hospital, Beijing 100853, ChinaMolecular & Immunological Department, Bio-therapeutic Department, Chinese PLA General Hospital, Beijing 100853, ChinaMolecular & Immunological Department, Bio-therapeutic Department, Chinese PLA General Hospital, Beijing 100853, ChinaMolecular & Immunological Department, Bio-therapeutic Department, Chinese PLA General Hospital, Beijing 100853, ChinaMolecular & Immunological Department, Bio-therapeutic Department, Chinese PLA General Hospital, Beijing 100853, ChinaMolecular & Immunological Department, Bio-therapeutic Department, Chinese PLA General Hospital, Beijing 100853, ChinaMedical School of Nankai University, Tianjin 300071, ChinaMolecular & Immunological Department, Bio-therapeutic Department, Chinese PLA General Hospital, Beijing 100853, ChinaBurns Institute, The First Affiliated Hospital of Chinese PLA General Hospital, Beijing 100853, ChinaMolecular & Immunological Department, Bio-therapeutic Department, Chinese PLA General Hospital, Beijing 100853, ChinaVolumetric muscle loss (VML) injury resulted from massive muscle defects and diseases for which there are still no effective therapeutic treatments. This study aimed to investigate the effects of rat adipose-derived mesenchymal stem cells (rASCs) and rASCs-conditioned medium- (CM-) based type I collagen hydrogel on macrophage (MP) transition, myogenesis, and vascularization in the rat VML model. Laser Doppler results demonstrated much higher blood flow in the rASC- and CM-based hydrogel groups. qRT-PCR, hematoxylin and eosin, immunofluorescence, and Sirius Red staining manifested that both rASCs and CM-based hydrogel implantation accelerated muscle repair with upregulated angiogenesis and myogenesis, attenuated inflammation while facilitating M2 transition, and decreased the collagen deposition compared with the hydrogel group. In vitro experiments indicated that factors secreted from polarized M2 MPs could accelerate the migration and tube formation capacities of HUVECs. These results suggested that rASCs exerted immunomodulatory effects on MPs which further enhanced the proangiogenic potential on ECs to promote myogenesis and angiogenesis during muscle repair. These fundamental results support further clinical applications of ASCs for muscle loss injury.http://dx.doi.org/10.1155/2017/2896874 |
| spellingShingle | Hong Huang Jiejie Liu Haojie Hao Deyun Chen Ling Zhizhong Meirong Li Haijing Song Rong Xiang Chaoguang Jiang Xiaobing Fu Weidong Han Preferred M2 Polarization by ASC-Based Hydrogel Accelerated Angiogenesis and Myogenesis in Volumetric Muscle Loss Rats Stem Cells International |
| title | Preferred M2 Polarization by ASC-Based Hydrogel Accelerated Angiogenesis and Myogenesis in Volumetric Muscle Loss Rats |
| title_full | Preferred M2 Polarization by ASC-Based Hydrogel Accelerated Angiogenesis and Myogenesis in Volumetric Muscle Loss Rats |
| title_fullStr | Preferred M2 Polarization by ASC-Based Hydrogel Accelerated Angiogenesis and Myogenesis in Volumetric Muscle Loss Rats |
| title_full_unstemmed | Preferred M2 Polarization by ASC-Based Hydrogel Accelerated Angiogenesis and Myogenesis in Volumetric Muscle Loss Rats |
| title_short | Preferred M2 Polarization by ASC-Based Hydrogel Accelerated Angiogenesis and Myogenesis in Volumetric Muscle Loss Rats |
| title_sort | preferred m2 polarization by asc based hydrogel accelerated angiogenesis and myogenesis in volumetric muscle loss rats |
| url | http://dx.doi.org/10.1155/2017/2896874 |
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