Whole genome-sequence analysis of Bacillus subtilis strain KC14-1 with broad-spectrum antifungal activity
Abstract Background Bacillus is used as a biological control agent in agricultural production. The main mechanisms responsible for its biocontrol activity encompass the generation of various antifungal active substances during life activities, competition, antagonism with pathogens, promotion of gro...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-03-01
|
| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-025-11227-3 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849733365914140672 |
|---|---|
| author | Xiaowei Li Yahan Chen Shunyi Yang Yi Zhou Chengde Yang |
| author_facet | Xiaowei Li Yahan Chen Shunyi Yang Yi Zhou Chengde Yang |
| author_sort | Xiaowei Li |
| collection | DOAJ |
| description | Abstract Background Bacillus is used as a biological control agent in agricultural production. The main mechanisms responsible for its biocontrol activity encompass the generation of various antifungal active substances during life activities, competition, antagonism with pathogens, promotion of growth, and induction of plant resistance, enhancing the inhibition of pathogenic fungi. Bacillus has high biological control potential and has become a research hotspot. Results It was found that strain KC14-1 had significant inhibitory effects on Fusarium fujikuroi, Rhizoclonia solani, Alternaria solani, Fusarium oxysporum, and Valsa mali. Based on morphological observations, physiological and biochemical determinations, and 16 S rRNA, gyrA, and gyrB gene sequencing, strain KC14-1 was identified as Bacillus subtilis. Whole genome sequencing results showed that the genome of strain KC14-1 was composed of a ring chromosome 3,908,079 bp in size, with a GC content of 43.82% and 3,895 coding genes. Anti-SMASH predicted that the genome of strain KC14-1 contained nine gene clusters that synthesised antibacterial substances. The homology between fengycin, bacillibactin, pulcherriminic acid, subtilosin A, and bacilysin was 100%. Conclusion The biocontrol potential of Bacillus subtilis KC14-1 was determined through whole-genome analysis. Our study provides a solid foundation for developing and utilising this strain. |
| format | Article |
| id | doaj-art-8a1ca75839604243b14ccd4f382599b0 |
| institution | DOAJ |
| issn | 1471-2164 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj-art-8a1ca75839604243b14ccd4f382599b02025-08-20T03:08:02ZengBMCBMC Genomics1471-21642025-03-0126111410.1186/s12864-025-11227-3Whole genome-sequence analysis of Bacillus subtilis strain KC14-1 with broad-spectrum antifungal activityXiaowei Li0Yahan Chen1Shunyi Yang2Yi Zhou3Chengde Yang4College of Plant Protection, Gansu Agricultural UniversityCollege of Plant Protection, Gansu Agricultural UniversityCollege of Plant Protection, Gansu Agricultural UniversityCollege of Plant Protection, Gansu Agricultural UniversityCollege of Plant Protection, Gansu Agricultural UniversityAbstract Background Bacillus is used as a biological control agent in agricultural production. The main mechanisms responsible for its biocontrol activity encompass the generation of various antifungal active substances during life activities, competition, antagonism with pathogens, promotion of growth, and induction of plant resistance, enhancing the inhibition of pathogenic fungi. Bacillus has high biological control potential and has become a research hotspot. Results It was found that strain KC14-1 had significant inhibitory effects on Fusarium fujikuroi, Rhizoclonia solani, Alternaria solani, Fusarium oxysporum, and Valsa mali. Based on morphological observations, physiological and biochemical determinations, and 16 S rRNA, gyrA, and gyrB gene sequencing, strain KC14-1 was identified as Bacillus subtilis. Whole genome sequencing results showed that the genome of strain KC14-1 was composed of a ring chromosome 3,908,079 bp in size, with a GC content of 43.82% and 3,895 coding genes. Anti-SMASH predicted that the genome of strain KC14-1 contained nine gene clusters that synthesised antibacterial substances. The homology between fengycin, bacillibactin, pulcherriminic acid, subtilosin A, and bacilysin was 100%. Conclusion The biocontrol potential of Bacillus subtilis KC14-1 was determined through whole-genome analysis. Our study provides a solid foundation for developing and utilising this strain.https://doi.org/10.1186/s12864-025-11227-3Whole genome sequenceBacillus subtilisSecondary metabolitesKC14-1 |
| spellingShingle | Xiaowei Li Yahan Chen Shunyi Yang Yi Zhou Chengde Yang Whole genome-sequence analysis of Bacillus subtilis strain KC14-1 with broad-spectrum antifungal activity BMC Genomics Whole genome sequence Bacillus subtilis Secondary metabolites KC14-1 |
| title | Whole genome-sequence analysis of Bacillus subtilis strain KC14-1 with broad-spectrum antifungal activity |
| title_full | Whole genome-sequence analysis of Bacillus subtilis strain KC14-1 with broad-spectrum antifungal activity |
| title_fullStr | Whole genome-sequence analysis of Bacillus subtilis strain KC14-1 with broad-spectrum antifungal activity |
| title_full_unstemmed | Whole genome-sequence analysis of Bacillus subtilis strain KC14-1 with broad-spectrum antifungal activity |
| title_short | Whole genome-sequence analysis of Bacillus subtilis strain KC14-1 with broad-spectrum antifungal activity |
| title_sort | whole genome sequence analysis of bacillus subtilis strain kc14 1 with broad spectrum antifungal activity |
| topic | Whole genome sequence Bacillus subtilis Secondary metabolites KC14-1 |
| url | https://doi.org/10.1186/s12864-025-11227-3 |
| work_keys_str_mv | AT xiaoweili wholegenomesequenceanalysisofbacillussubtilisstrainkc141withbroadspectrumantifungalactivity AT yahanchen wholegenomesequenceanalysisofbacillussubtilisstrainkc141withbroadspectrumantifungalactivity AT shunyiyang wholegenomesequenceanalysisofbacillussubtilisstrainkc141withbroadspectrumantifungalactivity AT yizhou wholegenomesequenceanalysisofbacillussubtilisstrainkc141withbroadspectrumantifungalactivity AT chengdeyang wholegenomesequenceanalysisofbacillussubtilisstrainkc141withbroadspectrumantifungalactivity |