Simultaneous Determination of Nitroimidazoles and Quinolones in Honey by Modified QuEChERS and LC-MS/MS Analysis
This study reports an analytical method for the determination of nitroimidazole and quinolones in honey using liquid chromatography-tandem mass spectrometry (LC-MS/MS). A modified QuEChERS methodology was used to extract the analytes and determine veterinary drugs in honey by LC-MS/MS. The linear re...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/4271385 |
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| author | Haiyan Lei Jianbo Guo Zhuo Lv Xiaohong Zhu Xiaofeng Xue Liming Wu Wei Cao |
| author_facet | Haiyan Lei Jianbo Guo Zhuo Lv Xiaohong Zhu Xiaofeng Xue Liming Wu Wei Cao |
| author_sort | Haiyan Lei |
| collection | DOAJ |
| description | This study reports an analytical method for the determination of nitroimidazole and quinolones in honey using liquid chromatography-tandem mass spectrometry (LC-MS/MS). A modified QuEChERS methodology was used to extract the analytes and determine veterinary drugs in honey by LC-MS/MS. The linear regression was excellent at the concentration levels of 1–100 ng/mL in the solution standard curve and the matrix standard curve. The recovery rates of nitroimidazole and quinolones were 4.4% to 59.1% and 9.8% to 46.2% with relative standard deviations (RSDs) below 5.2% and the recovery rates of nitroimidazole and quinolones by the matrix standard curve ranged from 82.0% to 117.8% and 79% to 115.9% with relative standard deviations (RSDs) lower than 6.3% in acacia and jujube honey. The acacia and jujube honeys have stronger matrix inhibition effect to nitroimidazole and quinolones residue; the matrix inhibition effect of jujube honey is stronger than acacia honey. The matrix standard curve can calibrate matrix effect effectively. In this study, the detection method of antibiotics in honey can be applied to the actual sample. The results demonstrated that the modified QuEChERS method combined with LC-MS/MS is a rapid, high, sensitive method for the analysis of nitroimidazoles and quinolones residues in honey. |
| format | Article |
| id | doaj-art-8a0bc3ed7a0e4b1a9908eaa19ac24ad2 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-8a0bc3ed7a0e4b1a9908eaa19ac24ad22025-08-20T03:37:51ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792018-01-01201810.1155/2018/42713854271385Simultaneous Determination of Nitroimidazoles and Quinolones in Honey by Modified QuEChERS and LC-MS/MS AnalysisHaiyan Lei0Jianbo Guo1Zhuo Lv2Xiaohong Zhu3Xiaofeng Xue4Liming Wu5Wei Cao6Department of Food Science and Engineering, School of Chemical Engineering, Northwest University, Xi’an, Shaanxi 710069, ChinaShaanxi Institute for Food and Drug Control, Xi’an, Shaanxi 710069, ChinaShaanxi Institute for Food and Drug Control, Xi’an, Shaanxi 710069, ChinaShaanxi Institute for Food and Drug Control, Xi’an, Shaanxi 710069, ChinaInstitute of Apiculture Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaInstitute of Apiculture Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaDepartment of Food Science and Engineering, School of Chemical Engineering, Northwest University, Xi’an, Shaanxi 710069, ChinaThis study reports an analytical method for the determination of nitroimidazole and quinolones in honey using liquid chromatography-tandem mass spectrometry (LC-MS/MS). A modified QuEChERS methodology was used to extract the analytes and determine veterinary drugs in honey by LC-MS/MS. The linear regression was excellent at the concentration levels of 1–100 ng/mL in the solution standard curve and the matrix standard curve. The recovery rates of nitroimidazole and quinolones were 4.4% to 59.1% and 9.8% to 46.2% with relative standard deviations (RSDs) below 5.2% and the recovery rates of nitroimidazole and quinolones by the matrix standard curve ranged from 82.0% to 117.8% and 79% to 115.9% with relative standard deviations (RSDs) lower than 6.3% in acacia and jujube honey. The acacia and jujube honeys have stronger matrix inhibition effect to nitroimidazole and quinolones residue; the matrix inhibition effect of jujube honey is stronger than acacia honey. The matrix standard curve can calibrate matrix effect effectively. In this study, the detection method of antibiotics in honey can be applied to the actual sample. The results demonstrated that the modified QuEChERS method combined with LC-MS/MS is a rapid, high, sensitive method for the analysis of nitroimidazoles and quinolones residues in honey.http://dx.doi.org/10.1155/2018/4271385 |
| spellingShingle | Haiyan Lei Jianbo Guo Zhuo Lv Xiaohong Zhu Xiaofeng Xue Liming Wu Wei Cao Simultaneous Determination of Nitroimidazoles and Quinolones in Honey by Modified QuEChERS and LC-MS/MS Analysis International Journal of Analytical Chemistry |
| title | Simultaneous Determination of Nitroimidazoles and Quinolones in Honey by Modified QuEChERS and LC-MS/MS Analysis |
| title_full | Simultaneous Determination of Nitroimidazoles and Quinolones in Honey by Modified QuEChERS and LC-MS/MS Analysis |
| title_fullStr | Simultaneous Determination of Nitroimidazoles and Quinolones in Honey by Modified QuEChERS and LC-MS/MS Analysis |
| title_full_unstemmed | Simultaneous Determination of Nitroimidazoles and Quinolones in Honey by Modified QuEChERS and LC-MS/MS Analysis |
| title_short | Simultaneous Determination of Nitroimidazoles and Quinolones in Honey by Modified QuEChERS and LC-MS/MS Analysis |
| title_sort | simultaneous determination of nitroimidazoles and quinolones in honey by modified quechers and lc ms ms analysis |
| url | http://dx.doi.org/10.1155/2018/4271385 |
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