Riboflavin/UVA collagen cross-linking-induced changes in normal and keratoconus corneal stroma.
<h4>Purpose</h4>To determine the effect of Ultraviolet-A collagen cross-linking with hypo-osmolar and iso-osmolar riboflavin solutions on stromal collagen ultrastructure in normal and keratoconus ex vivo human corneas.<h4>Methods</h4>Using small-angle X-ray scattering, measur...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0022405 |
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| author | Sally Hayes Craig Boote Christina S Kamma-Lorger Madhavan S Rajan Jonathan Harris Erin Dooley Nicholas Hawksworth Jennifer Hiller Nick J Terill Farhad Hafezi Arun K Brahma Andrew J Quantock Keith M Meek |
| author_facet | Sally Hayes Craig Boote Christina S Kamma-Lorger Madhavan S Rajan Jonathan Harris Erin Dooley Nicholas Hawksworth Jennifer Hiller Nick J Terill Farhad Hafezi Arun K Brahma Andrew J Quantock Keith M Meek |
| author_sort | Sally Hayes |
| collection | DOAJ |
| description | <h4>Purpose</h4>To determine the effect of Ultraviolet-A collagen cross-linking with hypo-osmolar and iso-osmolar riboflavin solutions on stromal collagen ultrastructure in normal and keratoconus ex vivo human corneas.<h4>Methods</h4>Using small-angle X-ray scattering, measurements of collagen D-periodicity, fibril diameter and interfibrillar spacing were made at 1 mm intervals across six normal post-mortem corneas (two above physiological hydration (swollen) and four below (unswollen)) and two post-transplant keratoconus corneal buttons (one swollen; one unswollen), before and after hypo-osmolar cross-linking. The same parameters were measured in three other unswollen normal corneas before and after iso-osmolar cross-linking and in three pairs of swollen normal corneas, in which only the left was cross-linked (with iso-osmolar riboflavin).<h4>Results</h4>Hypo-osmolar cross-linking resulted in an increase in corneal hydration in all corneas. In the keratoconus corneas and unswollen normal corneas, this was accompanied by an increase in collagen interfibrillar spacing (p<0.001); an increase in fibril diameter was also seen in two out of four unswollen normal corneas and one unswollen keratoconus cornea (p<0.001). Iso-osmolar cross-linking resulted in a decrease in tissue hydration in the swollen normal corneas only. Although there was no consistent treatment-induced change in hydration in the unswollen normal samples, iso-osmolar cross-linking of these corneas did result in a compaction of collagen fibrils and a reduced fibril diameter (p<0.001); these changes were not seen in the swollen normal corneas. Collagen D-periodicity was not affected by either treatment.<h4>Conclusion</h4>The observed structural changes following Ultraviolet-A cross-linking with hypo-osmolar or iso-osmolar riboflavin solutions are more likely a consequence of treatment-induced changes in tissue hydration rather than cross-linking. |
| format | Article |
| id | doaj-art-89fddd5996a94c0ebac34ce4b5a4beef |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-89fddd5996a94c0ebac34ce4b5a4beef2025-08-20T02:08:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0168e2240510.1371/journal.pone.0022405Riboflavin/UVA collagen cross-linking-induced changes in normal and keratoconus corneal stroma.Sally HayesCraig BooteChristina S Kamma-LorgerMadhavan S RajanJonathan HarrisErin DooleyNicholas HawksworthJennifer HillerNick J TerillFarhad HafeziArun K BrahmaAndrew J QuantockKeith M Meek<h4>Purpose</h4>To determine the effect of Ultraviolet-A collagen cross-linking with hypo-osmolar and iso-osmolar riboflavin solutions on stromal collagen ultrastructure in normal and keratoconus ex vivo human corneas.<h4>Methods</h4>Using small-angle X-ray scattering, measurements of collagen D-periodicity, fibril diameter and interfibrillar spacing were made at 1 mm intervals across six normal post-mortem corneas (two above physiological hydration (swollen) and four below (unswollen)) and two post-transplant keratoconus corneal buttons (one swollen; one unswollen), before and after hypo-osmolar cross-linking. The same parameters were measured in three other unswollen normal corneas before and after iso-osmolar cross-linking and in three pairs of swollen normal corneas, in which only the left was cross-linked (with iso-osmolar riboflavin).<h4>Results</h4>Hypo-osmolar cross-linking resulted in an increase in corneal hydration in all corneas. In the keratoconus corneas and unswollen normal corneas, this was accompanied by an increase in collagen interfibrillar spacing (p<0.001); an increase in fibril diameter was also seen in two out of four unswollen normal corneas and one unswollen keratoconus cornea (p<0.001). Iso-osmolar cross-linking resulted in a decrease in tissue hydration in the swollen normal corneas only. Although there was no consistent treatment-induced change in hydration in the unswollen normal samples, iso-osmolar cross-linking of these corneas did result in a compaction of collagen fibrils and a reduced fibril diameter (p<0.001); these changes were not seen in the swollen normal corneas. Collagen D-periodicity was not affected by either treatment.<h4>Conclusion</h4>The observed structural changes following Ultraviolet-A cross-linking with hypo-osmolar or iso-osmolar riboflavin solutions are more likely a consequence of treatment-induced changes in tissue hydration rather than cross-linking.https://doi.org/10.1371/journal.pone.0022405 |
| spellingShingle | Sally Hayes Craig Boote Christina S Kamma-Lorger Madhavan S Rajan Jonathan Harris Erin Dooley Nicholas Hawksworth Jennifer Hiller Nick J Terill Farhad Hafezi Arun K Brahma Andrew J Quantock Keith M Meek Riboflavin/UVA collagen cross-linking-induced changes in normal and keratoconus corneal stroma. PLoS ONE |
| title | Riboflavin/UVA collagen cross-linking-induced changes in normal and keratoconus corneal stroma. |
| title_full | Riboflavin/UVA collagen cross-linking-induced changes in normal and keratoconus corneal stroma. |
| title_fullStr | Riboflavin/UVA collagen cross-linking-induced changes in normal and keratoconus corneal stroma. |
| title_full_unstemmed | Riboflavin/UVA collagen cross-linking-induced changes in normal and keratoconus corneal stroma. |
| title_short | Riboflavin/UVA collagen cross-linking-induced changes in normal and keratoconus corneal stroma. |
| title_sort | riboflavin uva collagen cross linking induced changes in normal and keratoconus corneal stroma |
| url | https://doi.org/10.1371/journal.pone.0022405 |
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