Investigating the Bioactive Potential of Ononis spinosa L. Phytochemical Profiling and Antibacterial Effectiveness Evaluation
This study was conducted in order to investigate the antibacterial potency of the Ononis spinosa L. extracts. The phenolic compounds in the aerial parts of Ononis spinosa were examined using high performance liquid chromatography coupled with a diode array detector (HPLC-UV/DAD) technology, and were...
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2025-03-01
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| Series: | Novel Research in Microbiology Journal |
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| author | Lakhdar Benalach Fadhela Boukada Kouider Cherifi Ali Latreche Ikram Messellem Mohammed Bellatreche |
| author_facet | Lakhdar Benalach Fadhela Boukada Kouider Cherifi Ali Latreche Ikram Messellem Mohammed Bellatreche |
| author_sort | Lakhdar Benalach |
| collection | DOAJ |
| description | This study was conducted in order to investigate the antibacterial potency of the Ononis spinosa L. extracts. The phenolic compounds in the aerial parts of Ononis spinosa were examined using high performance liquid chromatography coupled with a diode array detector (HPLC-UV/DAD) technology, and were assessed due to their antimicrobial properties. The greatest total phenolic amount of the extracts, as measured by the Folin-Ciocalteu reagent procedure, was found to be 43.03 ± 1.02 mg GAE/gDW in stem extract, while the utmost flavonoid content value was 31.45 ± 0.45 mg QE/gDW in stem extract, as measured by aluminum chloride colorimetric method. Based on HPLC analysis, we find that epicatechin (5.1 mg/g) constituted the predominant compound in the aerial plant extract. The antibacterial capability of the extracts was determined against twelve strains of Gram-negative and Gram-positive bacteria. The strains evaluated were chosen for an antibacterial assay utilizing the in vitro disc diffusion and the minimum inhibitory concentration (MIC) assays. All examined extracts exhibited antibacterial potential, varying from moderate to strong, with the stem extract demonstrating the most pronounced effect, significantly inhibiting the development of Staphylococcus aureus recording diameter of inhibition zone, MIC, and MBC (minimal bactericidal concentration) of 30 mm, 4.68 mg/ ml, and 9.36 mg/ ml, respectively. The extracts from Ononis spinosa showed a high antibacterial efficacy, thus they may have potential applications in food and pharmaceutical products. |
| format | Article |
| id | doaj-art-89fd2be41b5244cc9064271a830fe8f1 |
| institution | DOAJ |
| issn | 2537-0286 2537-0294 |
| language | English |
| publishDate | 2025-03-01 |
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| series | Novel Research in Microbiology Journal |
| spelling | doaj-art-89fd2be41b5244cc9064271a830fe8f12025-08-20T02:40:48ZengResearchersLinks, LtdNovel Research in Microbiology Journal2537-02862537-02942025-03-01926372https://dx.doi.org/10.17582/journal.NRMJ/2025/9.2.82.91Investigating the Bioactive Potential of Ononis spinosa L. Phytochemical Profiling and Antibacterial Effectiveness EvaluationLakhdar Benalach0Fadhela Boukada1Kouider Cherifi2Ali Latreche3Ikram Messellem4Mohammed Bellatreche5Laboratory of Plant Biodiversity: Conservation and Valorization, Faculty of Natural Sciences and Life, University of Djillali Liabes, Sidi Bel Abbès 22000, AlgeriaLaboratory of Research, Bioconversion, Engineering Microbiology and Health Safety, Faculty of Science of the Nature and Life, University of Mascara (UN 2901), BP. 763, Sidi Said, Mascara, AlgeriaLaboratory of Plant Biodiversity: Conservation and Valorization, Faculty of Natural Sciences and Life, University of Djillali Liabes, Sidi Bel Abbès 22000, AlgeriaLaboratory of Plant Biodiversity: Conservation and Valorization, Faculty of Natural Sciences and Life, University of Djillali Liabes, Sidi Bel Abbès 22000, AlgeriaLaboratory of Biosystematics and Arthropod Ecology, Faculty of Natural and Life Sciences, Department of Animal Biology, Mentouri Constantine Brothers University, AlgeriaNational Institute for Plant Protection, El-Harache, Algeria.This study was conducted in order to investigate the antibacterial potency of the Ononis spinosa L. extracts. The phenolic compounds in the aerial parts of Ononis spinosa were examined using high performance liquid chromatography coupled with a diode array detector (HPLC-UV/DAD) technology, and were assessed due to their antimicrobial properties. The greatest total phenolic amount of the extracts, as measured by the Folin-Ciocalteu reagent procedure, was found to be 43.03 ± 1.02 mg GAE/gDW in stem extract, while the utmost flavonoid content value was 31.45 ± 0.45 mg QE/gDW in stem extract, as measured by aluminum chloride colorimetric method. Based on HPLC analysis, we find that epicatechin (5.1 mg/g) constituted the predominant compound in the aerial plant extract. The antibacterial capability of the extracts was determined against twelve strains of Gram-negative and Gram-positive bacteria. The strains evaluated were chosen for an antibacterial assay utilizing the in vitro disc diffusion and the minimum inhibitory concentration (MIC) assays. All examined extracts exhibited antibacterial potential, varying from moderate to strong, with the stem extract demonstrating the most pronounced effect, significantly inhibiting the development of Staphylococcus aureus recording diameter of inhibition zone, MIC, and MBC (minimal bactericidal concentration) of 30 mm, 4.68 mg/ ml, and 9.36 mg/ ml, respectively. The extracts from Ononis spinosa showed a high antibacterial efficacy, thus they may have potential applications in food and pharmaceutical products.https://researcherslinks.com/current-issues/Investigating-the-Bioactive-Potential-of-Ononis/44/1/10970/htmlononis spinosaantibacterial potencyhplcpolyphenolsflavonoidsmic |
| spellingShingle | Lakhdar Benalach Fadhela Boukada Kouider Cherifi Ali Latreche Ikram Messellem Mohammed Bellatreche Investigating the Bioactive Potential of Ononis spinosa L. Phytochemical Profiling and Antibacterial Effectiveness Evaluation Novel Research in Microbiology Journal ononis spinosa antibacterial potency hplc polyphenols flavonoids mic |
| title | Investigating the Bioactive Potential of Ononis spinosa L. Phytochemical Profiling and Antibacterial Effectiveness Evaluation |
| title_full | Investigating the Bioactive Potential of Ononis spinosa L. Phytochemical Profiling and Antibacterial Effectiveness Evaluation |
| title_fullStr | Investigating the Bioactive Potential of Ononis spinosa L. Phytochemical Profiling and Antibacterial Effectiveness Evaluation |
| title_full_unstemmed | Investigating the Bioactive Potential of Ononis spinosa L. Phytochemical Profiling and Antibacterial Effectiveness Evaluation |
| title_short | Investigating the Bioactive Potential of Ononis spinosa L. Phytochemical Profiling and Antibacterial Effectiveness Evaluation |
| title_sort | investigating the bioactive potential of ononis spinosa l phytochemical profiling and antibacterial effectiveness evaluation |
| topic | ononis spinosa antibacterial potency hplc polyphenols flavonoids mic |
| url | https://researcherslinks.com/current-issues/Investigating-the-Bioactive-Potential-of-Ononis/44/1/10970/html |
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