Collagen Content in Skin and Internal Organs of the Tight Skin Mouse: An Animal Model of Scleroderma
The Tight Skin mouse is a genetically induced animal model of tissue fibrosis caused by a large in-frame mutation in the gene encoding fibrillin-1 (Fbn-1). We examined the influence of gender on the collagen content of tissues in C57BL/6J wild type (+/+) and mutant Tight Skin (Tsk/+) mice employing...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
|
| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2013/436053 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850212724202536960 |
|---|---|
| author | Jayanthi Manne Marina Markova Linda D. Siracusa Sergio A. Jimenez |
| author_facet | Jayanthi Manne Marina Markova Linda D. Siracusa Sergio A. Jimenez |
| author_sort | Jayanthi Manne |
| collection | DOAJ |
| description | The Tight Skin mouse is a genetically induced animal model of tissue fibrosis caused by a large in-frame mutation in the gene encoding fibrillin-1 (Fbn-1). We examined the influence of gender on the collagen content of tissues in C57BL/6J wild type (+/+) and mutant Tight Skin (Tsk/+) mice employing hydroxyproline assays. Tissue sections were stained with Masson’s trichrome to identify collagen in situ. Adult Tsk/+ mice skin contains ~15% more collagen, on average, than skin from +/+ mice of the same gender. The heart of Tsk/+ males had significantly more collagen than that of +/+ males. No significant gender differences were found in lungs and kidney collagen content. Overall, the collagen content of Tsk/+ males and +/+ males was higher than that of their Tsk/+ and +/+ female counterparts, respectively. Our data confirm increased deposition of collagen in skin and hearts of Tsk/+ mice; however, the effects of the Tsk mutation on collagen content are both tissue specific and gender specific. These results indicate that comparative studies of collagen content between normal and Tsk/+ mice skin and internal organs must take into account gender differences caused by expression of the androgen receptor. |
| format | Article |
| id | doaj-art-89f7bb4e5f93450da30ce9f42dcb9727 |
| institution | OA Journals |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-89f7bb4e5f93450da30ce9f42dcb97272025-08-20T02:09:17ZengWileyBiochemistry Research International2090-22472090-22552013-01-01201310.1155/2013/436053436053Collagen Content in Skin and Internal Organs of the Tight Skin Mouse: An Animal Model of SclerodermaJayanthi Manne0Marina Markova1Linda D. Siracusa2Sergio A. Jimenez3Department of Microbiology and Immunology, Kimmel Cancer Center, Jefferson Medical College, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107-5541, USADepartment of Microbiology and Immunology, Kimmel Cancer Center, Jefferson Medical College, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107-5541, USADepartment of Microbiology and Immunology, Kimmel Cancer Center, Jefferson Medical College, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107-5541, USAJefferson Institute of Molecular Medicine, Jefferson Medical College, Thomas Jefferson University, Suite 509 Bluemle Life Sciences Building, 233 South 10th Street, Philadelphia, PA 19107-5541, USAThe Tight Skin mouse is a genetically induced animal model of tissue fibrosis caused by a large in-frame mutation in the gene encoding fibrillin-1 (Fbn-1). We examined the influence of gender on the collagen content of tissues in C57BL/6J wild type (+/+) and mutant Tight Skin (Tsk/+) mice employing hydroxyproline assays. Tissue sections were stained with Masson’s trichrome to identify collagen in situ. Adult Tsk/+ mice skin contains ~15% more collagen, on average, than skin from +/+ mice of the same gender. The heart of Tsk/+ males had significantly more collagen than that of +/+ males. No significant gender differences were found in lungs and kidney collagen content. Overall, the collagen content of Tsk/+ males and +/+ males was higher than that of their Tsk/+ and +/+ female counterparts, respectively. Our data confirm increased deposition of collagen in skin and hearts of Tsk/+ mice; however, the effects of the Tsk mutation on collagen content are both tissue specific and gender specific. These results indicate that comparative studies of collagen content between normal and Tsk/+ mice skin and internal organs must take into account gender differences caused by expression of the androgen receptor.http://dx.doi.org/10.1155/2013/436053 |
| spellingShingle | Jayanthi Manne Marina Markova Linda D. Siracusa Sergio A. Jimenez Collagen Content in Skin and Internal Organs of the Tight Skin Mouse: An Animal Model of Scleroderma Biochemistry Research International |
| title | Collagen Content in Skin and Internal Organs of the Tight Skin Mouse: An Animal Model of Scleroderma |
| title_full | Collagen Content in Skin and Internal Organs of the Tight Skin Mouse: An Animal Model of Scleroderma |
| title_fullStr | Collagen Content in Skin and Internal Organs of the Tight Skin Mouse: An Animal Model of Scleroderma |
| title_full_unstemmed | Collagen Content in Skin and Internal Organs of the Tight Skin Mouse: An Animal Model of Scleroderma |
| title_short | Collagen Content in Skin and Internal Organs of the Tight Skin Mouse: An Animal Model of Scleroderma |
| title_sort | collagen content in skin and internal organs of the tight skin mouse an animal model of scleroderma |
| url | http://dx.doi.org/10.1155/2013/436053 |
| work_keys_str_mv | AT jayanthimanne collagencontentinskinandinternalorgansofthetightskinmouseananimalmodelofscleroderma AT marinamarkova collagencontentinskinandinternalorgansofthetightskinmouseananimalmodelofscleroderma AT lindadsiracusa collagencontentinskinandinternalorgansofthetightskinmouseananimalmodelofscleroderma AT sergioajimenez collagencontentinskinandinternalorgansofthetightskinmouseananimalmodelofscleroderma |