Symptomatic, widespread, and inconspicuous: new detection of tomato fruit blotch virus
Tomato production is an important part of the Swiss vegetable production with most tomato crops grown in greenhouses. Tomato plants are vulnerable to diseases caused by viruses, which can have significant impacts on crop production. This study reports the first detection of tomato fruit botch virus...
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| Format: | Article |
| Language: | English |
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Firenze University Press
2023-09-01
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| Series: | Phytopathologia Mediterranea |
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| Online Access: | https://oajournals.fupress.net/index.php/pm/article/view/14463 |
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| author | Arnaud G. BLOUIN Nathalie DUBUIS Justine BRODARD Laure APOTHÉLOZ-PERRET-GENTIL Denise ALTENBACH Olivier SCHUMPP |
| author_facet | Arnaud G. BLOUIN Nathalie DUBUIS Justine BRODARD Laure APOTHÉLOZ-PERRET-GENTIL Denise ALTENBACH Olivier SCHUMPP |
| author_sort | Arnaud G. BLOUIN |
| collection | DOAJ |
| description | Tomato production is an important part of the Swiss vegetable production with most tomato crops grown in greenhouses. Tomato plants are vulnerable to diseases caused by viruses, which can have significant impacts on crop production. This study reports the first detection of tomato fruit botch virus (ToFBV, Blunervirus solani) in Switzerland, from a tomato production site at the southern part of the Ticino region. The symptoms observed indicated presence of a viral pathogen, but tests against the most common tomato viruses were negative. Immunocapture of double-stranded RNA and its subsequent sequencing on a Flongle flowcell (Oxford Nanopore Technologies) identified the presence of ToFBV and southern tomato virus. The genome of the Swiss ToFBV isolate was very similar to that available in GenBank. Datamining of the sequence read archives found the virus in two other countries, with a highly conserved genome. With this study, there are now 12 near-complete genomes of ToFBV available, and the virus is recorded from ten countries. This study underlines the importance of continuous monitoring and research on emerging viruses in tomato production. |
| format | Article |
| id | doaj-art-89f5f5b68abc45018f42ca8dd9637c6e |
| institution | OA Journals |
| issn | 0031-9465 1593-2095 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Firenze University Press |
| record_format | Article |
| series | Phytopathologia Mediterranea |
| spelling | doaj-art-89f5f5b68abc45018f42ca8dd9637c6e2025-08-20T02:11:29ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952023-09-0162334935410.36253/phyto-1446313289Symptomatic, widespread, and inconspicuous: new detection of tomato fruit blotch virusArnaud G. BLOUIN0https://orcid.org/0000-0003-1360-1529Nathalie DUBUIS1Justine BRODARD2Laure APOTHÉLOZ-PERRET-GENTIL3https://orcid.org/0000-0002-8592-3079Denise ALTENBACH4Olivier SCHUMPP5https://orcid.org/0000-0002-2070-2144Virology-Phytoplasmology Laboratory, Agroscope, 1260 NyonVirology-Phytoplasmology Laboratory, Agroscope, 1260 NyonVirology-Phytoplasmology Laboratory, Agroscope, 1260 NyonDiagnostic moléculaire des organismes nuisibles réglementés des végétaux, Agroscope, 1260 NyonDiagnostic moléculaire des organismes nuisibles réglementés des végétaux, Agroscope, 1260 NyonVirology-Phytoplasmology Laboratory, Agroscope, 1260 NyonTomato production is an important part of the Swiss vegetable production with most tomato crops grown in greenhouses. Tomato plants are vulnerable to diseases caused by viruses, which can have significant impacts on crop production. This study reports the first detection of tomato fruit botch virus (ToFBV, Blunervirus solani) in Switzerland, from a tomato production site at the southern part of the Ticino region. The symptoms observed indicated presence of a viral pathogen, but tests against the most common tomato viruses were negative. Immunocapture of double-stranded RNA and its subsequent sequencing on a Flongle flowcell (Oxford Nanopore Technologies) identified the presence of ToFBV and southern tomato virus. The genome of the Swiss ToFBV isolate was very similar to that available in GenBank. Datamining of the sequence read archives found the virus in two other countries, with a highly conserved genome. With this study, there are now 12 near-complete genomes of ToFBV available, and the virus is recorded from ten countries. This study underlines the importance of continuous monitoring and research on emerging viruses in tomato production.https://oajournals.fupress.net/index.php/pm/article/view/14463 kitaviridaeblunervirus solaniflongle sequencingdsrna |
| spellingShingle | Arnaud G. BLOUIN Nathalie DUBUIS Justine BRODARD Laure APOTHÉLOZ-PERRET-GENTIL Denise ALTENBACH Olivier SCHUMPP Symptomatic, widespread, and inconspicuous: new detection of tomato fruit blotch virus Phytopathologia Mediterranea kitaviridae blunervirus solani flongle sequencing dsrna |
| title | Symptomatic, widespread, and inconspicuous: new detection of tomato fruit blotch virus |
| title_full | Symptomatic, widespread, and inconspicuous: new detection of tomato fruit blotch virus |
| title_fullStr | Symptomatic, widespread, and inconspicuous: new detection of tomato fruit blotch virus |
| title_full_unstemmed | Symptomatic, widespread, and inconspicuous: new detection of tomato fruit blotch virus |
| title_short | Symptomatic, widespread, and inconspicuous: new detection of tomato fruit blotch virus |
| title_sort | symptomatic widespread and inconspicuous new detection of tomato fruit blotch virus |
| topic | kitaviridae blunervirus solani flongle sequencing dsrna |
| url | https://oajournals.fupress.net/index.php/pm/article/view/14463 |
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