Protocol for processing and analyzing multiplexed images improves lymphatic cell identification and spatial architecture in human tissue

Protocol for processing and analyzing multiplexed images improves lymphatic cell identification and spatial architecture in human tissue

Summary: Multiplexed images of human lymphatic tissue are extensively preprocessed before cell phenotyping and spatial analysis. Here, we present KINTSUGI (knowledge integration with new technologies: simplified user-guided image processing), a protocol designed to interactively engage the user in e...

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Bibliographic Details
Main Authors: Justin A. Smith, Drew T. Bloss, MacKenzie D. Williams, Amanda L. Posgai, Todd M. Brusko, Mark A. Atkinson, Clive H. Wasserfall, Maigan A. Brusko
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
Subjects:
Immunology
Microscopy
Protein Biochemistry
Proteomics
Computer sciences
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672500382X
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