Screening Germplasms and Detecting Quantitative Trait Loci for High Sucrose Content in Soybean
Sucrose is a desirable component of processed soybean foods and animal feed, and thus, its content is used as an important characteristic for assessing the quality of soybean seeds. However, few studies have focused on the quantitative trait loci (QTLs) associated with sucrose regulation in soybean...
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MDPI AG
2024-10-01
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| Series: | Plants |
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| author | Se-Hee Kang Seo-Young Shin Byeong Hee Kang Sreeparna Chowdhury Won-Ho Lee Woon Ji Kim Jeong-Dong Lee Sungwoo Lee Yu-Mi Choi Bo-Keun Ha |
| author_facet | Se-Hee Kang Seo-Young Shin Byeong Hee Kang Sreeparna Chowdhury Won-Ho Lee Woon Ji Kim Jeong-Dong Lee Sungwoo Lee Yu-Mi Choi Bo-Keun Ha |
| author_sort | Se-Hee Kang |
| collection | DOAJ |
| description | Sucrose is a desirable component of processed soybean foods and animal feed, and thus, its content is used as an important characteristic for assessing the quality of soybean seeds. However, few studies have focused on the quantitative trait loci (QTLs) associated with sucrose regulation in soybean seeds. This study aims to measure the sucrose content of 1014 soybean accessions and identify genes related to high sucrose levels using QTL analysis. Colorimetric analysis based on the enzymatic reaction of invertase (INV) and glucose oxidase (GOD) was employed to test the germplasms. A total of six high-sucrose genetic resources (IT186230, IT195321, IT263138, IT263276, IT263286, and IT276521) and two low-sucrose genetic resources (IT025668 and IT274054) were identified. Two F<sub>2:3</sub> populations, IT186230 × IT025668 and Ilmi × IT186230, were then established from these germplasms. QTL analysis identified four QTLs (<i>qSUC6.1</i>, <i>qSUC11.1</i>, <i>qSUC15.1</i>, and <i>qSUC17.1</i>), explaining 7.3–27.6% of the phenotypic variation in the sugar content. Twenty candidate genes were found at the four QTLs. Notably, <i>Glyma.17G152300</i>, located in the <i>qSUC17.1</i> QTL region, exhibited a 17-fold higher gene expression in the high-sucrose germplasm IT186230 compared to the control germplasm Ilmi, confirming its role as a major gene regulating the sucrose content in soybean. These results may assist in marker-assisted selection for breeding programs that aim to develop soybean lines with a higher sucrose content. |
| format | Article |
| id | doaj-art-89e4503fc9b748eca82998f11d0ecd7d |
| institution | OA Journals |
| issn | 2223-7747 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj-art-89e4503fc9b748eca82998f11d0ecd7d2025-08-20T01:47:37ZengMDPI AGPlants2223-77472024-10-011319281510.3390/plants13192815Screening Germplasms and Detecting Quantitative Trait Loci for High Sucrose Content in SoybeanSe-Hee Kang0Seo-Young Shin1Byeong Hee Kang2Sreeparna Chowdhury3Won-Ho Lee4Woon Ji Kim5Jeong-Dong Lee6Sungwoo Lee7Yu-Mi Choi8Bo-Keun Ha9Department of Applied Plant Science, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Applied Plant Science, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Applied Plant Science, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Applied Plant Science, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Applied Plant Science, Chonnam National University, Gwangju 61186, Republic of KoreaAdvanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of KoreaDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Crop Science, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Republic of KoreaNational Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Jeonju 54874, Republic of KoreaDepartment of Applied Plant Science, Chonnam National University, Gwangju 61186, Republic of KoreaSucrose is a desirable component of processed soybean foods and animal feed, and thus, its content is used as an important characteristic for assessing the quality of soybean seeds. However, few studies have focused on the quantitative trait loci (QTLs) associated with sucrose regulation in soybean seeds. This study aims to measure the sucrose content of 1014 soybean accessions and identify genes related to high sucrose levels using QTL analysis. Colorimetric analysis based on the enzymatic reaction of invertase (INV) and glucose oxidase (GOD) was employed to test the germplasms. A total of six high-sucrose genetic resources (IT186230, IT195321, IT263138, IT263276, IT263286, and IT276521) and two low-sucrose genetic resources (IT025668 and IT274054) were identified. Two F<sub>2:3</sub> populations, IT186230 × IT025668 and Ilmi × IT186230, were then established from these germplasms. QTL analysis identified four QTLs (<i>qSUC6.1</i>, <i>qSUC11.1</i>, <i>qSUC15.1</i>, and <i>qSUC17.1</i>), explaining 7.3–27.6% of the phenotypic variation in the sugar content. Twenty candidate genes were found at the four QTLs. Notably, <i>Glyma.17G152300</i>, located in the <i>qSUC17.1</i> QTL region, exhibited a 17-fold higher gene expression in the high-sucrose germplasm IT186230 compared to the control germplasm Ilmi, confirming its role as a major gene regulating the sucrose content in soybean. These results may assist in marker-assisted selection for breeding programs that aim to develop soybean lines with a higher sucrose content.https://www.mdpi.com/2223-7747/13/19/2815soybeangermplasmsucroseglucose oxidaseinvertasequantitative trait locus |
| spellingShingle | Se-Hee Kang Seo-Young Shin Byeong Hee Kang Sreeparna Chowdhury Won-Ho Lee Woon Ji Kim Jeong-Dong Lee Sungwoo Lee Yu-Mi Choi Bo-Keun Ha Screening Germplasms and Detecting Quantitative Trait Loci for High Sucrose Content in Soybean Plants soybean germplasm sucrose glucose oxidase invertase quantitative trait locus |
| title | Screening Germplasms and Detecting Quantitative Trait Loci for High Sucrose Content in Soybean |
| title_full | Screening Germplasms and Detecting Quantitative Trait Loci for High Sucrose Content in Soybean |
| title_fullStr | Screening Germplasms and Detecting Quantitative Trait Loci for High Sucrose Content in Soybean |
| title_full_unstemmed | Screening Germplasms and Detecting Quantitative Trait Loci for High Sucrose Content in Soybean |
| title_short | Screening Germplasms and Detecting Quantitative Trait Loci for High Sucrose Content in Soybean |
| title_sort | screening germplasms and detecting quantitative trait loci for high sucrose content in soybean |
| topic | soybean germplasm sucrose glucose oxidase invertase quantitative trait locus |
| url | https://www.mdpi.com/2223-7747/13/19/2815 |
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