Influence of forage-to-concentrate ratio on the effects of a radiata pine bark extract on methane production and fermentation using the rumen simulation technique
Climate change and food safety standards have intensified research into plant-based compounds as alternatives to dietary supplements in animal feed. These compounds can reduce enteric methane (CH4) emissions and the formation of ruminal ammonia. This study investigated the effects of radiata pine ba...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-02-01
|
| Series: | Animal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1751731124003434 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823856839118290944 |
|---|---|
| author | N. Vera S. Suescun-Ospina C. Gutiérrez-Gómez P. Williams C. Fuentealba R. Allende J. Ávila-Stagno |
| author_facet | N. Vera S. Suescun-Ospina C. Gutiérrez-Gómez P. Williams C. Fuentealba R. Allende J. Ávila-Stagno |
| author_sort | N. Vera |
| collection | DOAJ |
| description | Climate change and food safety standards have intensified research into plant-based compounds as alternatives to dietary supplements in animal feed. These compounds can reduce enteric methane (CH4) emissions and the formation of ruminal ammonia. This study investigated the effects of radiata pine bark extract (PBE) supplementation on CH4 production, ruminal fermentation parameters, and nutrient disappearance using the rumen simulation technique in diets with different forage-to-concentrate (F:C) ratios. The experimental design was a 22 factorial arrangement of two F:C ratios [70:30 (HF) vs 30:70 (HC)], without or with PBE (2% on a DM basis). Two consecutive incubations were conducted during 15 days, with 10 days of adaptation, followed by 5 days of sampling. Use of PBE decreased ammonia nitrogen (NH3-N, P < 0.001) and total volatile fatty acids (VFAs, P = 0.019) concentrations, while it increased acetate (P < 0.001) and propionate (P < 0.001) proportions in both diets. However, CH4 yield [mg/g organic matter (OM) disappeared] tended to be reduced by 33.6% (P = 0.088) only in the HF diet supplemented with PBE. The OM disappearance tended to increase (P = 0.079) with PBE; hemicellulose disappearance increased in HF and decreased in HC diets (P < 0.001), whereas protein disappearance decreased in HF and increased in HC diets (P < 0.001). In conclusion, PBE supplementation reduced NH3-N and VFA concentrations in HF and HC diets, but CH4 yield (mg/g OM disappeared) was only reduced in the HF diet. Additionally, nutrient disappearance was dependent on the F:C ratio of the diet, without adverse effects on ruminal fermentation. Hence, the incorporation of radiata PBE into HF diets could serve as an environmentally friendly additive, potentially offering an alternative use for a forestry industry by-product, while beneficially modulating ruminal fermentation. |
| format | Article |
| id | doaj-art-89e41a85d9aa468eb78c18720c0aadca |
| institution | Kabale University |
| issn | 1751-7311 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Animal |
| spelling | doaj-art-89e41a85d9aa468eb78c18720c0aadca2025-02-12T05:30:53ZengElsevierAnimal1751-73112025-02-01192101406Influence of forage-to-concentrate ratio on the effects of a radiata pine bark extract on methane production and fermentation using the rumen simulation techniqueN. Vera0S. Suescun-Ospina1C. Gutiérrez-Gómez2P. Williams3C. Fuentealba4R. Allende5J. Ávila-Stagno6Department of Animal Science, Faculty of Veterinary Sciences, Universidad de Concepción, Campus Chillán, Chillán 3812120, ChileDepartment of Animal Production, Faculty of Agronomy, Universidad de Concepción, Campus Chillán, Chillán 3812120, ChileDepartment of Animal Science, Faculty of Veterinary Sciences, Universidad de Concepción, Campus Chillán, Chillán 3812120, ChileDepartment of Animal Production, Faculty of Agronomy, Universidad de Concepción, Campus Chillán, Chillán 3812120, ChileTechnological Development Unit, Universidad de Concepción, Coronel Industrial Park, Coronel 4190000, Chile; National Center of Excellence for the Timber Industry (CENAMAD), Pontificia Universidad Católica de Chile, Santiago 7820436, ChileDepartment of Animal Science, Faculty of Veterinary Sciences, Universidad de Concepción, Campus Chillán, Chillán 3812120, ChileDepartment of Animal Science, Faculty of Veterinary Sciences, Universidad de Concepción, Campus Chillán, Chillán 3812120, Chile; Corresponding author.Climate change and food safety standards have intensified research into plant-based compounds as alternatives to dietary supplements in animal feed. These compounds can reduce enteric methane (CH4) emissions and the formation of ruminal ammonia. This study investigated the effects of radiata pine bark extract (PBE) supplementation on CH4 production, ruminal fermentation parameters, and nutrient disappearance using the rumen simulation technique in diets with different forage-to-concentrate (F:C) ratios. The experimental design was a 22 factorial arrangement of two F:C ratios [70:30 (HF) vs 30:70 (HC)], without or with PBE (2% on a DM basis). Two consecutive incubations were conducted during 15 days, with 10 days of adaptation, followed by 5 days of sampling. Use of PBE decreased ammonia nitrogen (NH3-N, P < 0.001) and total volatile fatty acids (VFAs, P = 0.019) concentrations, while it increased acetate (P < 0.001) and propionate (P < 0.001) proportions in both diets. However, CH4 yield [mg/g organic matter (OM) disappeared] tended to be reduced by 33.6% (P = 0.088) only in the HF diet supplemented with PBE. The OM disappearance tended to increase (P = 0.079) with PBE; hemicellulose disappearance increased in HF and decreased in HC diets (P < 0.001), whereas protein disappearance decreased in HF and increased in HC diets (P < 0.001). In conclusion, PBE supplementation reduced NH3-N and VFA concentrations in HF and HC diets, but CH4 yield (mg/g OM disappeared) was only reduced in the HF diet. Additionally, nutrient disappearance was dependent on the F:C ratio of the diet, without adverse effects on ruminal fermentation. Hence, the incorporation of radiata PBE into HF diets could serve as an environmentally friendly additive, potentially offering an alternative use for a forestry industry by-product, while beneficially modulating ruminal fermentation.http://www.sciencedirect.com/science/article/pii/S1751731124003434Climate changeMitigationPhytochemicalsRuminantsTannins |
| spellingShingle | N. Vera S. Suescun-Ospina C. Gutiérrez-Gómez P. Williams C. Fuentealba R. Allende J. Ávila-Stagno Influence of forage-to-concentrate ratio on the effects of a radiata pine bark extract on methane production and fermentation using the rumen simulation technique Animal Climate change Mitigation Phytochemicals Ruminants Tannins |
| title | Influence of forage-to-concentrate ratio on the effects of a radiata pine bark extract on methane production and fermentation using the rumen simulation technique |
| title_full | Influence of forage-to-concentrate ratio on the effects of a radiata pine bark extract on methane production and fermentation using the rumen simulation technique |
| title_fullStr | Influence of forage-to-concentrate ratio on the effects of a radiata pine bark extract on methane production and fermentation using the rumen simulation technique |
| title_full_unstemmed | Influence of forage-to-concentrate ratio on the effects of a radiata pine bark extract on methane production and fermentation using the rumen simulation technique |
| title_short | Influence of forage-to-concentrate ratio on the effects of a radiata pine bark extract on methane production and fermentation using the rumen simulation technique |
| title_sort | influence of forage to concentrate ratio on the effects of a radiata pine bark extract on methane production and fermentation using the rumen simulation technique |
| topic | Climate change Mitigation Phytochemicals Ruminants Tannins |
| url | http://www.sciencedirect.com/science/article/pii/S1751731124003434 |
| work_keys_str_mv | AT nvera influenceofforagetoconcentrateratioontheeffectsofaradiatapinebarkextractonmethaneproductionandfermentationusingtherumensimulationtechnique AT ssuescunospina influenceofforagetoconcentrateratioontheeffectsofaradiatapinebarkextractonmethaneproductionandfermentationusingtherumensimulationtechnique AT cgutierrezgomez influenceofforagetoconcentrateratioontheeffectsofaradiatapinebarkextractonmethaneproductionandfermentationusingtherumensimulationtechnique AT pwilliams influenceofforagetoconcentrateratioontheeffectsofaradiatapinebarkextractonmethaneproductionandfermentationusingtherumensimulationtechnique AT cfuentealba influenceofforagetoconcentrateratioontheeffectsofaradiatapinebarkextractonmethaneproductionandfermentationusingtherumensimulationtechnique AT rallende influenceofforagetoconcentrateratioontheeffectsofaradiatapinebarkextractonmethaneproductionandfermentationusingtherumensimulationtechnique AT javilastagno influenceofforagetoconcentrateratioontheeffectsofaradiatapinebarkextractonmethaneproductionandfermentationusingtherumensimulationtechnique |