Immunohistochemical evaluation of podoplanin in odontogenic tumours & cysts using anti-human podoplanin antibody
Background: Odontogenic Cysts & tumors originate through some aberration from the normal pattern of odontogenesis. Ameloblastoma is one of the most frequent intraosseous odontogenic tumors. However it is no longer appropriate to use the diagnosis of ameloblastoma without specifying the type....
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Elsevier
2017-05-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221242681630152X |
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| author | Namrata Singhal Nitin Khanduri Deepak Kurup Brijesh Gupta Pranjan Mitra Roshani Chawla |
| author_facet | Namrata Singhal Nitin Khanduri Deepak Kurup Brijesh Gupta Pranjan Mitra Roshani Chawla |
| author_sort | Namrata Singhal |
| collection | DOAJ |
| description | Background: Odontogenic Cysts & tumors originate through some aberration from the normal pattern of odontogenesis. Ameloblastoma is one of the most frequent intraosseous odontogenic tumors. However it is no longer appropriate to use the diagnosis of ameloblastoma without specifying the type. Varied-clinical entities of ameloblastoma differ in their biologic behaviour. Odontogenic cysts like dentigerous and radicular cysts are less aggressive in nature than odontogenic tumors. Recently, podoplanin commonly used as a lymphatic endothelial marker in cancers has recently been found to play a possible role in odontogenic tumorigenesis also. Therefore the purpose of this study was to immunohistochemically analyse the expression of podoplanin in ameloblastomas, KCOTs, dentigerous cysts, radicular cysts & dental follicles. Methods: Paraffin-embedded tissue specimens of 15 Ameloblastomas (7 follicular, 6 unicystic, 2 desmoplastic),10KCOTs, 5 dentigerous cysts, 5 radicular cysts & 5 dental follicles were immunohistochemically examined using antibody against podoplanin. Results: All ameloblastomas displayed podoplanin expression in ameloblast-like cells of the epithelial islands while the stellate-reticulum like cells exhibited no or weak immunostaining. Expression of podoplanin in KCOTs was strongly positive in the cells of the basal and suprabasal layers & odontogenic epithelial nests. Positive immunoreaction for podoplanin was observed in the inflammatory radicular cysts and inflamed dentigerous cyst only and negative or weak expression in the lining epithelium of uninflamed dentigerous cysts and dental follicles. Conclusion: Our results suggest that podoplanin can be used as a potential proliferative marker to observe the aggressive behaviour of ameloblastomas and KCOTs. |
| format | Article |
| id | doaj-art-89e320dc5ccc4231bf04b7524a70b839 |
| institution | OA Journals |
| issn | 2212-4268 |
| language | English |
| publishDate | 2017-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-89e320dc5ccc4231bf04b7524a70b8392025-08-20T02:02:02ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682017-05-01729510010.1016/j.jobcr.2017.05.001Immunohistochemical evaluation of podoplanin in odontogenic tumours & cysts using anti-human podoplanin antibodyNamrata Singhal0Nitin Khanduri1Deepak Kurup2Brijesh Gupta3Pranjan Mitra4Roshani Chawla5Dept. of Oral Path, Institute of Dental Sciences & Advance Studies, Gwalior, MP, IndiaDept. of Pedo, Institute of Dental Sciences & Advance Studies, Gwalior, MP, IndiaDept. of Conservative, Institute of Dental Sciences & Advance Studies, Gwalior, MP, IndiaDept. of Oral surgery, Institute of Dental Sciences & Advance Studies, Gwalior, MP, IndiaDept. of Public Health Dentistry, Institute of Dental Sciences & Advance Studies, Gwalior, MP, IndiaDept. Of Public Health Dentistry, V.Y.W.S. Dental College, Amravati 444602, India; Corresponding author at: Senior Lecturer and Incharge Dept.Of Public Health Dentistry V.Y.W.S.Dental College, Amravati 444602, India.Background: Odontogenic Cysts & tumors originate through some aberration from the normal pattern of odontogenesis. Ameloblastoma is one of the most frequent intraosseous odontogenic tumors. However it is no longer appropriate to use the diagnosis of ameloblastoma without specifying the type. Varied-clinical entities of ameloblastoma differ in their biologic behaviour. Odontogenic cysts like dentigerous and radicular cysts are less aggressive in nature than odontogenic tumors. Recently, podoplanin commonly used as a lymphatic endothelial marker in cancers has recently been found to play a possible role in odontogenic tumorigenesis also. Therefore the purpose of this study was to immunohistochemically analyse the expression of podoplanin in ameloblastomas, KCOTs, dentigerous cysts, radicular cysts & dental follicles. Methods: Paraffin-embedded tissue specimens of 15 Ameloblastomas (7 follicular, 6 unicystic, 2 desmoplastic),10KCOTs, 5 dentigerous cysts, 5 radicular cysts & 5 dental follicles were immunohistochemically examined using antibody against podoplanin. Results: All ameloblastomas displayed podoplanin expression in ameloblast-like cells of the epithelial islands while the stellate-reticulum like cells exhibited no or weak immunostaining. Expression of podoplanin in KCOTs was strongly positive in the cells of the basal and suprabasal layers & odontogenic epithelial nests. Positive immunoreaction for podoplanin was observed in the inflammatory radicular cysts and inflamed dentigerous cyst only and negative or weak expression in the lining epithelium of uninflamed dentigerous cysts and dental follicles. Conclusion: Our results suggest that podoplanin can be used as a potential proliferative marker to observe the aggressive behaviour of ameloblastomas and KCOTs.http://www.sciencedirect.com/science/article/pii/S221242681630152XAmeloblastomaDentigerous cystKeratocystic odontogenic tumor (KCOT)Radicular cystPodoplanin |
| spellingShingle | Namrata Singhal Nitin Khanduri Deepak Kurup Brijesh Gupta Pranjan Mitra Roshani Chawla Immunohistochemical evaluation of podoplanin in odontogenic tumours & cysts using anti-human podoplanin antibody Journal of Oral Biology and Craniofacial Research Ameloblastoma Dentigerous cyst Keratocystic odontogenic tumor (KCOT) Radicular cyst Podoplanin |
| title | Immunohistochemical evaluation of podoplanin in odontogenic tumours & cysts using anti-human podoplanin antibody |
| title_full | Immunohistochemical evaluation of podoplanin in odontogenic tumours & cysts using anti-human podoplanin antibody |
| title_fullStr | Immunohistochemical evaluation of podoplanin in odontogenic tumours & cysts using anti-human podoplanin antibody |
| title_full_unstemmed | Immunohistochemical evaluation of podoplanin in odontogenic tumours & cysts using anti-human podoplanin antibody |
| title_short | Immunohistochemical evaluation of podoplanin in odontogenic tumours & cysts using anti-human podoplanin antibody |
| title_sort | immunohistochemical evaluation of podoplanin in odontogenic tumours amp cysts using anti human podoplanin antibody |
| topic | Ameloblastoma Dentigerous cyst Keratocystic odontogenic tumor (KCOT) Radicular cyst Podoplanin |
| url | http://www.sciencedirect.com/science/article/pii/S221242681630152X |
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