Pooled optical screening in bacteria using chromosomally expressed barcodes

Abstract Optical pooled screening is an important tool to study dynamic phenotypes for libraries of genetically engineered cells. However, the desired engineering often requires that the barcodes used for in situ genotyping are expressed from the chromosome. This has not previously been achieved in...

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Main Authors: Ruben R. G. Soares, Daniela A. García-Soriano, Jimmy Larsson, David Fange, Dvir Schirman, Marco Grillo, Anna Knöppel, Beer Chakra Sen, Fabian Svahn, Spartak Zikrin, Michael Ratz, Mats Nilsson, Johan Elf
Format: Article
Language:English
Published: Nature Portfolio 2025-06-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-025-08268-5
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