Transportation engineering for enhanced production of plant natural products in microbial cell factories

Plant natural products (PNPs) exhibit a wide range of biological activities and have essential applications in various fields such as medicine, agriculture, and flavors. Given their natural limitations, the production of high-value PNPs using microbial cell factories has become an effective alternat...

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Main Authors: Yimeng Zuo, Minghui Zhao, Yuanwei Gou, Lei Huang, Zhinan Xu, Jiazhang Lian
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2024-12-01
Series:Synthetic and Systems Biotechnology
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Online Access:http://www.sciencedirect.com/science/article/pii/S2405805X24000899
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author Yimeng Zuo
Minghui Zhao
Yuanwei Gou
Lei Huang
Zhinan Xu
Jiazhang Lian
author_facet Yimeng Zuo
Minghui Zhao
Yuanwei Gou
Lei Huang
Zhinan Xu
Jiazhang Lian
author_sort Yimeng Zuo
collection DOAJ
description Plant natural products (PNPs) exhibit a wide range of biological activities and have essential applications in various fields such as medicine, agriculture, and flavors. Given their natural limitations, the production of high-value PNPs using microbial cell factories has become an effective alternative in recent years. However, host metabolic burden caused by its massive accumulation has become one of the main challenges for efficient PNP production. Therefore, it is necessary to strengthen the transmembrane transport process of PNPs. This review introduces the discovery and mining of PNP transporters to directly mediate PNP transmembrane transportation both intracellularly and extracellularly. In addition to transporter engineering, this review also summarizes several auxiliary strategies (such as small molecules, environmental changes, and vesicles assisted transport) for strengthening PNP transportation. Finally, this review is concluded with the applications and future perspectives of transportation engineering in the construction and optimization of PNP microbial cell factories.
format Article
id doaj-art-89cdd451f9984554919a3d0dbddd36d1
institution Kabale University
issn 2405-805X
language English
publishDate 2024-12-01
publisher KeAi Communications Co., Ltd.
record_format Article
series Synthetic and Systems Biotechnology
spelling doaj-art-89cdd451f9984554919a3d0dbddd36d12024-12-15T06:16:03ZengKeAi Communications Co., Ltd.Synthetic and Systems Biotechnology2405-805X2024-12-0194742751Transportation engineering for enhanced production of plant natural products in microbial cell factoriesYimeng Zuo0Minghui Zhao1Yuanwei Gou2Lei Huang3Zhinan Xu4Jiazhang Lian5Key Laboratory of Biomass Chemical Engineering of Ministry of Education & National Key Laboratory of Biobased Transportation Fuel Technology, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China; ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, 310000, ChinaKey Laboratory of Biomass Chemical Engineering of Ministry of Education & National Key Laboratory of Biobased Transportation Fuel Technology, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China; ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, 310000, ChinaKey Laboratory of Biomass Chemical Engineering of Ministry of Education & National Key Laboratory of Biobased Transportation Fuel Technology, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China; ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, 310000, ChinaKey Laboratory of Biomass Chemical Engineering of Ministry of Education & National Key Laboratory of Biobased Transportation Fuel Technology, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China; ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, 310000, China; Corresponding author.Key Laboratory of Biomass Chemical Engineering of Ministry of Education & National Key Laboratory of Biobased Transportation Fuel Technology, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, ChinaKey Laboratory of Biomass Chemical Engineering of Ministry of Education & National Key Laboratory of Biobased Transportation Fuel Technology, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China; ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, 310000, China; Corresponding author.Key Laboratory of Biomass Chemical Engineering of Ministry of Education & National Key Laboratory of Biobased Transportation Fuel Technology, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, ChinaPlant natural products (PNPs) exhibit a wide range of biological activities and have essential applications in various fields such as medicine, agriculture, and flavors. Given their natural limitations, the production of high-value PNPs using microbial cell factories has become an effective alternative in recent years. However, host metabolic burden caused by its massive accumulation has become one of the main challenges for efficient PNP production. Therefore, it is necessary to strengthen the transmembrane transport process of PNPs. This review introduces the discovery and mining of PNP transporters to directly mediate PNP transmembrane transportation both intracellularly and extracellularly. In addition to transporter engineering, this review also summarizes several auxiliary strategies (such as small molecules, environmental changes, and vesicles assisted transport) for strengthening PNP transportation. Finally, this review is concluded with the applications and future perspectives of transportation engineering in the construction and optimization of PNP microbial cell factories.http://www.sciencedirect.com/science/article/pii/S2405805X24000899Plant natural productsTransportation engineeringTransportersMicrobial cell factories
spellingShingle Yimeng Zuo
Minghui Zhao
Yuanwei Gou
Lei Huang
Zhinan Xu
Jiazhang Lian
Transportation engineering for enhanced production of plant natural products in microbial cell factories
Synthetic and Systems Biotechnology
Plant natural products
Transportation engineering
Transporters
Microbial cell factories
title Transportation engineering for enhanced production of plant natural products in microbial cell factories
title_full Transportation engineering for enhanced production of plant natural products in microbial cell factories
title_fullStr Transportation engineering for enhanced production of plant natural products in microbial cell factories
title_full_unstemmed Transportation engineering for enhanced production of plant natural products in microbial cell factories
title_short Transportation engineering for enhanced production of plant natural products in microbial cell factories
title_sort transportation engineering for enhanced production of plant natural products in microbial cell factories
topic Plant natural products
Transportation engineering
Transporters
Microbial cell factories
url http://www.sciencedirect.com/science/article/pii/S2405805X24000899
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AT minghuizhao transportationengineeringforenhancedproductionofplantnaturalproductsinmicrobialcellfactories
AT yuanweigou transportationengineeringforenhancedproductionofplantnaturalproductsinmicrobialcellfactories
AT leihuang transportationengineeringforenhancedproductionofplantnaturalproductsinmicrobialcellfactories
AT zhinanxu transportationengineeringforenhancedproductionofplantnaturalproductsinmicrobialcellfactories
AT jiazhanglian transportationengineeringforenhancedproductionofplantnaturalproductsinmicrobialcellfactories