Enhanced Glutathione Production in <i>Saccharomyces cerevisiae</i> by High-Throughput Screening System Based on Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis
In this study, we established a mutagenesis and high-throughput screening system to select a high-yielding glutathione (GSH)-producing strain of <i>Saccharomyces cerevisiae</i>. The parent strain was mutated by atmospheric and room temperature plasma (ARTP) technology and cultivated usin...
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MDPI AG
2025-04-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/11/4/220 |
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| author | Lan Li Zejian Wang Ali Mohsin Yingping Zhuang |
| author_facet | Lan Li Zejian Wang Ali Mohsin Yingping Zhuang |
| author_sort | Lan Li |
| collection | DOAJ |
| description | In this study, we established a mutagenesis and high-throughput screening system to select a high-yielding glutathione (GSH)-producing strain of <i>Saccharomyces cerevisiae</i>. The parent strain was mutated by atmospheric and room temperature plasma (ARTP) technology and cultivated using ethionine plate cultivation. Subsequently, high-throughput screening was performed using liquid deep microtiter plates (MTPs) for cultivation and a microplate reader for rapid GSH detection. The results demonstrated the successful selection of a stable mutant strain, S-272, which exhibited significantly enhanced GSH production. Fermentation validation in 5 L bioreactors revealed that S-272 achieved a 14.7% higher final GSH concentration and a 19.5% higher intracellular GSH content compared to the parent strain. The improved performance of S-272 was attributed to enhanced ethanol utilization, elevated activity of γ-glutamylcysteine synthetase (γ-GCS), and increased intracellular trehalose content. This study presents an effective strategy for developing high GSH-yield strains using ARTP complex mutagenesis technology combined with high-throughput screening. |
| format | Article |
| id | doaj-art-89cc239977674a8dbe07809cd74c74b7 |
| institution | OA Journals |
| issn | 2311-5637 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj-art-89cc239977674a8dbe07809cd74c74b72025-08-20T02:28:28ZengMDPI AGFermentation2311-56372025-04-0111422010.3390/fermentation11040220Enhanced Glutathione Production in <i>Saccharomyces cerevisiae</i> by High-Throughput Screening System Based on Atmospheric and Room Temperature Plasma (ARTP) MutagenesisLan Li0Zejian Wang1Ali Mohsin2Yingping Zhuang3State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, ChinaIn this study, we established a mutagenesis and high-throughput screening system to select a high-yielding glutathione (GSH)-producing strain of <i>Saccharomyces cerevisiae</i>. The parent strain was mutated by atmospheric and room temperature plasma (ARTP) technology and cultivated using ethionine plate cultivation. Subsequently, high-throughput screening was performed using liquid deep microtiter plates (MTPs) for cultivation and a microplate reader for rapid GSH detection. The results demonstrated the successful selection of a stable mutant strain, S-272, which exhibited significantly enhanced GSH production. Fermentation validation in 5 L bioreactors revealed that S-272 achieved a 14.7% higher final GSH concentration and a 19.5% higher intracellular GSH content compared to the parent strain. The improved performance of S-272 was attributed to enhanced ethanol utilization, elevated activity of γ-glutamylcysteine synthetase (γ-GCS), and increased intracellular trehalose content. This study presents an effective strategy for developing high GSH-yield strains using ARTP complex mutagenesis technology combined with high-throughput screening.https://www.mdpi.com/2311-5637/11/4/220glutathione (GSH)<i>Saccharomyces cerevisiae</i>high-throughput screeningatmospheric and room temperature plasma (ARTP)fermentation |
| spellingShingle | Lan Li Zejian Wang Ali Mohsin Yingping Zhuang Enhanced Glutathione Production in <i>Saccharomyces cerevisiae</i> by High-Throughput Screening System Based on Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis Fermentation glutathione (GSH) <i>Saccharomyces cerevisiae</i> high-throughput screening atmospheric and room temperature plasma (ARTP) fermentation |
| title | Enhanced Glutathione Production in <i>Saccharomyces cerevisiae</i> by High-Throughput Screening System Based on Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis |
| title_full | Enhanced Glutathione Production in <i>Saccharomyces cerevisiae</i> by High-Throughput Screening System Based on Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis |
| title_fullStr | Enhanced Glutathione Production in <i>Saccharomyces cerevisiae</i> by High-Throughput Screening System Based on Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis |
| title_full_unstemmed | Enhanced Glutathione Production in <i>Saccharomyces cerevisiae</i> by High-Throughput Screening System Based on Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis |
| title_short | Enhanced Glutathione Production in <i>Saccharomyces cerevisiae</i> by High-Throughput Screening System Based on Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis |
| title_sort | enhanced glutathione production in i saccharomyces cerevisiae i by high throughput screening system based on atmospheric and room temperature plasma artp mutagenesis |
| topic | glutathione (GSH) <i>Saccharomyces cerevisiae</i> high-throughput screening atmospheric and room temperature plasma (ARTP) fermentation |
| url | https://www.mdpi.com/2311-5637/11/4/220 |
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