The immune NIK1/RPL10/LIMYB signaling module regulates photosynthesis and translation under biotic and abiotic stresses.

Abstract Photosynthesis and translation are targets of metabolic control and development in plants, yet how stress signals coordinately regulate these opposing energy-producing and consuming processes remains enigmatic. Here, we unravel a growth control circuit that ties photosynthesis to translatio...

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Main Authors: Marco Aurélio Ferreira, Ruan M. Teixeira, Otávio J. B. Brustolini, Thainá F. F. Saia, James Jean-Baptiste, Nathalia G. A. Ribeiro, Sâmera S. Breves, Fellipe R. Sampaio, Eulálio G. D. Santos, Borys A. Leon, Celio C. Oliveira, Christiane E. M. Duarte, Lucas L. Lima, Leandro L. Oliveira, Humberto J. O. Ramos, Pedro A. B. Reis, Elizabeth P. B. Fontes
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-59571-y
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author Marco Aurélio Ferreira
Ruan M. Teixeira
Otávio J. B. Brustolini
Thainá F. F. Saia
James Jean-Baptiste
Nathalia G. A. Ribeiro
Sâmera S. Breves
Fellipe R. Sampaio
Eulálio G. D. Santos
Borys A. Leon
Celio C. Oliveira
Christiane E. M. Duarte
Lucas L. Lima
Leandro L. Oliveira
Humberto J. O. Ramos
Pedro A. B. Reis
Elizabeth P. B. Fontes
author_facet Marco Aurélio Ferreira
Ruan M. Teixeira
Otávio J. B. Brustolini
Thainá F. F. Saia
James Jean-Baptiste
Nathalia G. A. Ribeiro
Sâmera S. Breves
Fellipe R. Sampaio
Eulálio G. D. Santos
Borys A. Leon
Celio C. Oliveira
Christiane E. M. Duarte
Lucas L. Lima
Leandro L. Oliveira
Humberto J. O. Ramos
Pedro A. B. Reis
Elizabeth P. B. Fontes
author_sort Marco Aurélio Ferreira
collection DOAJ
description Abstract Photosynthesis and translation are targets of metabolic control and development in plants, yet how stress signals coordinately regulate these opposing energy-producing and consuming processes remains enigmatic. Here, we unravel a growth control circuit that ties photosynthesis to translational control in response to biotic and abiotic signals. Our findings reveal that the L10-INTERACTING MYB DOMAIN-CONTAINING PROTEIN (LIMYB), a key player of the NUCLEAR SHUTTLE PROTEIN-INTERACTING KINASE 1 (NIK1)/ RIBOSOMAL PROTEIN L10 (RPL10) antiviral signaling pathway, not only downregulates translation genes, but also represses photosynthesis-related genes and photosynthesis itself. LIMYB repressor activity, regulated by phosphorylation, is crucial for the decline in photosynthesis under stress. NIK1 activation by PAMPs or the phosphomimetic NIK1-T474D represses photosynthesis-related genes and photosynthesis in control but not in limyb lines. Furthermore, heat and osmotic stress also activate the NIK1/RPL10/LIMYB signaling circuit in wild type. These stresses induce NIK1 phosphorylation, but not marker gene repression, in limyb, indicating that LIMYB connects NIK1 activation to stress-mediated downregulation of translation- and photosynthesis-related genes. This coordinated repression via the NIK1/RPL10/LIMYB module may help plants adapt to changing environments.
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spelling doaj-art-89b32bc7434549cdb64b18e99b2d09652025-08-20T02:25:08ZengNature PortfolioNature Communications2041-17232025-05-0116111910.1038/s41467-025-59571-yThe immune NIK1/RPL10/LIMYB signaling module regulates photosynthesis and translation under biotic and abiotic stresses.Marco Aurélio Ferreira0Ruan M. Teixeira1Otávio J. B. Brustolini2Thainá F. F. Saia3James Jean-Baptiste4Nathalia G. A. Ribeiro5Sâmera S. Breves6Fellipe R. Sampaio7Eulálio G. D. Santos8Borys A. Leon9Celio C. Oliveira10Christiane E. M. Duarte11Lucas L. Lima12Leandro L. Oliveira13Humberto J. O. Ramos14Pedro A. B. Reis15Elizabeth P. B. Fontes16Department of Biochemistry and Molecular Biology, Universidade Federal de ViçosaDepartment of Biochemistry and Molecular Biology, Universidade Federal de ViçosaDepartment of Biochemistry and Molecular Biology, Universidade Federal de ViçosaNational Institute of Science and Technology in Plant-Pest Interactions, Bioagro, Universidade Federal de ViçosaNational Institute of Science and Technology in Plant-Pest Interactions, Bioagro, Universidade Federal de ViçosaNational Institute of Science and Technology in Plant-Pest Interactions, Bioagro, Universidade Federal de ViçosaNational Institute of Science and Technology in Plant-Pest Interactions, Bioagro, Universidade Federal de ViçosaNational Institute of Science and Technology in Plant-Pest Interactions, Bioagro, Universidade Federal de ViçosaDepartment of Biochemistry and Molecular Biology, Universidade Federal de ViçosaNational Institute of Science and Technology in Plant-Pest Interactions, Bioagro, Universidade Federal de ViçosaBrazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM)National Institute of Science and Technology in Plant-Pest Interactions, Bioagro, Universidade Federal de ViçosaDepartment of Biochemistry and Molecular Biology, Universidade Federal de ViçosaDepartment of General Biology, Universidade Federal de ViçosaDepartment of Biochemistry and Molecular Biology, Universidade Federal de ViçosaDepartment of Biochemistry and Molecular Biology, Universidade Federal de ViçosaDepartment of Biochemistry and Molecular Biology, Universidade Federal de ViçosaAbstract Photosynthesis and translation are targets of metabolic control and development in plants, yet how stress signals coordinately regulate these opposing energy-producing and consuming processes remains enigmatic. Here, we unravel a growth control circuit that ties photosynthesis to translational control in response to biotic and abiotic signals. Our findings reveal that the L10-INTERACTING MYB DOMAIN-CONTAINING PROTEIN (LIMYB), a key player of the NUCLEAR SHUTTLE PROTEIN-INTERACTING KINASE 1 (NIK1)/ RIBOSOMAL PROTEIN L10 (RPL10) antiviral signaling pathway, not only downregulates translation genes, but also represses photosynthesis-related genes and photosynthesis itself. LIMYB repressor activity, regulated by phosphorylation, is crucial for the decline in photosynthesis under stress. NIK1 activation by PAMPs or the phosphomimetic NIK1-T474D represses photosynthesis-related genes and photosynthesis in control but not in limyb lines. Furthermore, heat and osmotic stress also activate the NIK1/RPL10/LIMYB signaling circuit in wild type. These stresses induce NIK1 phosphorylation, but not marker gene repression, in limyb, indicating that LIMYB connects NIK1 activation to stress-mediated downregulation of translation- and photosynthesis-related genes. This coordinated repression via the NIK1/RPL10/LIMYB module may help plants adapt to changing environments.https://doi.org/10.1038/s41467-025-59571-y
spellingShingle Marco Aurélio Ferreira
Ruan M. Teixeira
Otávio J. B. Brustolini
Thainá F. F. Saia
James Jean-Baptiste
Nathalia G. A. Ribeiro
Sâmera S. Breves
Fellipe R. Sampaio
Eulálio G. D. Santos
Borys A. Leon
Celio C. Oliveira
Christiane E. M. Duarte
Lucas L. Lima
Leandro L. Oliveira
Humberto J. O. Ramos
Pedro A. B. Reis
Elizabeth P. B. Fontes
The immune NIK1/RPL10/LIMYB signaling module regulates photosynthesis and translation under biotic and abiotic stresses.
Nature Communications
title The immune NIK1/RPL10/LIMYB signaling module regulates photosynthesis and translation under biotic and abiotic stresses.
title_full The immune NIK1/RPL10/LIMYB signaling module regulates photosynthesis and translation under biotic and abiotic stresses.
title_fullStr The immune NIK1/RPL10/LIMYB signaling module regulates photosynthesis and translation under biotic and abiotic stresses.
title_full_unstemmed The immune NIK1/RPL10/LIMYB signaling module regulates photosynthesis and translation under biotic and abiotic stresses.
title_short The immune NIK1/RPL10/LIMYB signaling module regulates photosynthesis and translation under biotic and abiotic stresses.
title_sort immune nik1 rpl10 limyb signaling module regulates photosynthesis and translation under biotic and abiotic stresses
url https://doi.org/10.1038/s41467-025-59571-y
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