Challenges in lentiviral vector production: retro-transduction of producer cell lines
The increasing demand for lentiviral vectors (LVs) has led to the development of several stable cell lines and production methods over the last 2 decades in order to increase titers and yields, reduce production costs and improve the safety of the vector product. However, the phenomenon of retro-tra...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1569298/full |
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| author | Maximilian Klimpel Christin Obert Monica Terrao Parameswari Singh Haiyan Wei Chao-Guang Chen Andreas Gille Silke Wissing Holger Laux |
| author_facet | Maximilian Klimpel Christin Obert Monica Terrao Parameswari Singh Haiyan Wei Chao-Guang Chen Andreas Gille Silke Wissing Holger Laux |
| author_sort | Maximilian Klimpel |
| collection | DOAJ |
| description | The increasing demand for lentiviral vectors (LVs) has led to the development of several stable cell lines and production methods over the last 2 decades in order to increase titers and yields, reduce production costs and improve the safety of the vector product. However, the phenomenon of retro-transduction, which describes the transduction of LV producer cells by self-produced LVs, remains largely unaddressed in the context of LV production. Recent research has focused on various approaches to reduce the impact of retro-transduction on LV yield and process performance. This article reviews existing and new research data that highlights the impact of retro-transduction in LV manufacturing. In addition, a perspective on current advances to reduce retro-transduction is provided and a potential novel strategy called ENV-Y is presented, which could not only reduce the impact of retro-transduction but also facilitate the subsequent LV downstream process. |
| format | Article |
| id | doaj-art-89aea5a92f4a4b548b0e2dcf038b8d3c |
| institution | DOAJ |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-89aea5a92f4a4b548b0e2dcf038b8d3c2025-08-20T03:22:19ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-05-011310.3389/fbioe.2025.15692981569298Challenges in lentiviral vector production: retro-transduction of producer cell linesMaximilian Klimpel0Christin Obert1Monica Terrao2Parameswari Singh3Haiyan Wei4Chao-Guang Chen5Andreas Gille6Silke Wissing7Holger Laux8CSL Innovation GmbH, Marburg, GermanyCSL Innovation GmbH, Marburg, GermanyCSL Innovation GmbH, Marburg, GermanyCSL Innovation GmbH, Marburg, GermanyCSL Innovation, Melbourne, AustraliaCSL Innovation, Melbourne, AustraliaCSL Behring, Pasadena, CA, United StatesCSL Innovation GmbH, Marburg, GermanyCSL Innovation GmbH, Marburg, GermanyThe increasing demand for lentiviral vectors (LVs) has led to the development of several stable cell lines and production methods over the last 2 decades in order to increase titers and yields, reduce production costs and improve the safety of the vector product. However, the phenomenon of retro-transduction, which describes the transduction of LV producer cells by self-produced LVs, remains largely unaddressed in the context of LV production. Recent research has focused on various approaches to reduce the impact of retro-transduction on LV yield and process performance. This article reviews existing and new research data that highlights the impact of retro-transduction in LV manufacturing. In addition, a perspective on current advances to reduce retro-transduction is provided and a potential novel strategy called ENV-Y is presented, which could not only reduce the impact of retro-transduction but also facilitate the subsequent LV downstream process.https://www.frontiersin.org/articles/10.3389/fbioe.2025.1569298/fulllentiviral vector (LV)viral vector productionretro-transductionauto-transductioncell and gene therapy (CGT)stable producer cell line |
| spellingShingle | Maximilian Klimpel Christin Obert Monica Terrao Parameswari Singh Haiyan Wei Chao-Guang Chen Andreas Gille Silke Wissing Holger Laux Challenges in lentiviral vector production: retro-transduction of producer cell lines Frontiers in Bioengineering and Biotechnology lentiviral vector (LV) viral vector production retro-transduction auto-transduction cell and gene therapy (CGT) stable producer cell line |
| title | Challenges in lentiviral vector production: retro-transduction of producer cell lines |
| title_full | Challenges in lentiviral vector production: retro-transduction of producer cell lines |
| title_fullStr | Challenges in lentiviral vector production: retro-transduction of producer cell lines |
| title_full_unstemmed | Challenges in lentiviral vector production: retro-transduction of producer cell lines |
| title_short | Challenges in lentiviral vector production: retro-transduction of producer cell lines |
| title_sort | challenges in lentiviral vector production retro transduction of producer cell lines |
| topic | lentiviral vector (LV) viral vector production retro-transduction auto-transduction cell and gene therapy (CGT) stable producer cell line |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1569298/full |
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