Krill oil ameliorates benign prostatic hyperplasia by regulating G1-phase cell cycle arrest and altering signaling pathways and benign prostatic hyperplasia-associated markers
Krill oil (KO) exhibits various biological activities, such as anti-inflammatory and antitumor effects. However, the inhibitory effects of benign prostatic hyperplasia (BPH) in vitro and in vivo have not yet been studied. This study investigated the anti-BPH effects of KO extracted by an enzymatic h...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2024-11-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2023.9250017 |
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| author | Hoon Kim Jongyeob Kim Byungdoo Hwang SangYong Park Ji-Yeon Shin EunByeol Go Jae Sil Kim Youngjin Roh SoonChul Myung Seok-Joong Yun YungHyun Choi Wun-Jae Kim Sung-Kwon Moon |
| author_facet | Hoon Kim Jongyeob Kim Byungdoo Hwang SangYong Park Ji-Yeon Shin EunByeol Go Jae Sil Kim Youngjin Roh SoonChul Myung Seok-Joong Yun YungHyun Choi Wun-Jae Kim Sung-Kwon Moon |
| author_sort | Hoon Kim |
| collection | DOAJ |
| description | Krill oil (KO) exhibits various biological activities, such as anti-inflammatory and antitumor effects. However, the inhibitory effects of benign prostatic hyperplasia (BPH) in vitro and in vivo have not yet been studied. This study investigated the anti-BPH effects of KO extracted by an enzymatic hydrolysis method. KO treatment inhibited the proliferation of WMPY-1 and BPH-1 cells by induction of G0/G1 phase arrest through the modulation of positive and negative regulators in both prostate cell types. KO treatment stimulated phosphorylation of c-Jun N-terminal kinase (JNK) and p38 signaling. In addition, KO changed the expression of BPH-related markers (5α-reductase, androgen receptor, FGF, Bcl-2, and Bax) and the activity of the proliferation-mediated NF-κB binding motif. KO-induced levels of proliferation-mediated molecules of prostate cells were attenuated in the presence of siRNA-specific p-38 (si-p38) and JNK (si-JNK). Furthermore, the administration of KO alleviated prostate size and weight and the cell layer thickness of prostate glands in a testosterone enanthate-induced BPH rat model. KO treatment altered the level of dihydrotestosterone in serum and the expression levels of BPH-related markers in prostate tissues. Finally, KO-mediated inhibition of prostatic growth was validated by histological analysis. These results suggest that KO has an inhibitory effect on BPH in prostate cells in vitro and in vivo. Thus, KO might be a potential prophylactic or therapeutic agent for patients with BPH. |
| format | Article |
| id | doaj-art-89aa4fc9a0cc471e8c6c4423eef38ec5 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-89aa4fc9a0cc471e8c6c4423eef38ec52025-01-10T06:57:02ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-11-011363311332410.26599/FSHW.2023.9250017Krill oil ameliorates benign prostatic hyperplasia by regulating G1-phase cell cycle arrest and altering signaling pathways and benign prostatic hyperplasia-associated markersHoon Kim0Jongyeob Kim1Byungdoo Hwang2SangYong Park3Ji-Yeon Shin4EunByeol Go5Jae Sil Kim6Youngjin Roh7SoonChul Myung8Seok-Joong Yun9YungHyun Choi10Wun-Jae Kim11Sung-Kwon Moon12Department of Food and Nutrition, Chung-Ang University, Anseong 17546, Republic of KoreaDepartment of Food and Nutrition, Chung-Ang University, Anseong 17546, Republic of KoreaDepartment of Food and Nutrition, Chung-Ang University, Anseong 17546, Republic of KoreaSD Biotechnologies, Seoul 07793, Republic of KoreaSD Biotechnologies, Seoul 07793, Republic of KoreaSD Biotechnologies, Seoul 07793, Republic of KoreaSD Biotechnologies, Seoul 07793, Republic of KoreaDepartment of Food and Nutrition, Chung-Ang University, Anseong 17546, Republic of KoreaDepartment of Urology, College of Medicine, Chung-Ang University, Seoul 06974, Republic of KoreaDepartment of Urology, Chungbuk National University, Chungbuk 361-763, Republic of KoreaDepartment of Biochemistry, College of Oriental Medicine, Dongeui University, Busan 47340, Republic of KoreaInstitute of Urotech, Cheongju, Chungcheongbuk-do 361-763, Republic of KoreaDepartment of Food and Nutrition, Chung-Ang University, Anseong 17546, Republic of KoreaKrill oil (KO) exhibits various biological activities, such as anti-inflammatory and antitumor effects. However, the inhibitory effects of benign prostatic hyperplasia (BPH) in vitro and in vivo have not yet been studied. This study investigated the anti-BPH effects of KO extracted by an enzymatic hydrolysis method. KO treatment inhibited the proliferation of WMPY-1 and BPH-1 cells by induction of G0/G1 phase arrest through the modulation of positive and negative regulators in both prostate cell types. KO treatment stimulated phosphorylation of c-Jun N-terminal kinase (JNK) and p38 signaling. In addition, KO changed the expression of BPH-related markers (5α-reductase, androgen receptor, FGF, Bcl-2, and Bax) and the activity of the proliferation-mediated NF-κB binding motif. KO-induced levels of proliferation-mediated molecules of prostate cells were attenuated in the presence of siRNA-specific p-38 (si-p38) and JNK (si-JNK). Furthermore, the administration of KO alleviated prostate size and weight and the cell layer thickness of prostate glands in a testosterone enanthate-induced BPH rat model. KO treatment altered the level of dihydrotestosterone in serum and the expression levels of BPH-related markers in prostate tissues. Finally, KO-mediated inhibition of prostatic growth was validated by histological analysis. These results suggest that KO has an inhibitory effect on BPH in prostate cells in vitro and in vivo. Thus, KO might be a potential prophylactic or therapeutic agent for patients with BPH.https://www.sciopen.com/article/10.26599/FSHW.2023.9250017proliferationg0/g1-phase cell cyclenf-κbdihydrotestosterone |
| spellingShingle | Hoon Kim Jongyeob Kim Byungdoo Hwang SangYong Park Ji-Yeon Shin EunByeol Go Jae Sil Kim Youngjin Roh SoonChul Myung Seok-Joong Yun YungHyun Choi Wun-Jae Kim Sung-Kwon Moon Krill oil ameliorates benign prostatic hyperplasia by regulating G1-phase cell cycle arrest and altering signaling pathways and benign prostatic hyperplasia-associated markers Food Science and Human Wellness proliferation g0/g1-phase cell cycle nf-κb dihydrotestosterone |
| title | Krill oil ameliorates benign prostatic hyperplasia by regulating G1-phase cell cycle arrest and altering signaling pathways and benign prostatic hyperplasia-associated markers |
| title_full | Krill oil ameliorates benign prostatic hyperplasia by regulating G1-phase cell cycle arrest and altering signaling pathways and benign prostatic hyperplasia-associated markers |
| title_fullStr | Krill oil ameliorates benign prostatic hyperplasia by regulating G1-phase cell cycle arrest and altering signaling pathways and benign prostatic hyperplasia-associated markers |
| title_full_unstemmed | Krill oil ameliorates benign prostatic hyperplasia by regulating G1-phase cell cycle arrest and altering signaling pathways and benign prostatic hyperplasia-associated markers |
| title_short | Krill oil ameliorates benign prostatic hyperplasia by regulating G1-phase cell cycle arrest and altering signaling pathways and benign prostatic hyperplasia-associated markers |
| title_sort | krill oil ameliorates benign prostatic hyperplasia by regulating g1 phase cell cycle arrest and altering signaling pathways and benign prostatic hyperplasia associated markers |
| topic | proliferation g0/g1-phase cell cycle nf-κb dihydrotestosterone |
| url | https://www.sciopen.com/article/10.26599/FSHW.2023.9250017 |
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