An immunoregulation PLGA/Chitosan aligned nanofibers with polydopamine coupling basic fibroblast growth factor and ROS scavenging for peripheral nerve regeneration
The repair and functional recovery of long-segment peripheral nerve injuries are crucial in clinical settings. Nerve conduits are seen as promising alternatives to autologous nerve grafts, but their effectiveness is limited by the controlled delivery of bioactive factors and meeting various function...
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Elsevier
2025-04-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425001012 |
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| author | Xiaokun Chen Jihai Xu Feng Qin Ziyuan Yang Xueyuan Li Miao Yu Ming Li Yanhua Wang Wang Xin |
| author_facet | Xiaokun Chen Jihai Xu Feng Qin Ziyuan Yang Xueyuan Li Miao Yu Ming Li Yanhua Wang Wang Xin |
| author_sort | Xiaokun Chen |
| collection | DOAJ |
| description | The repair and functional recovery of long-segment peripheral nerve injuries are crucial in clinical settings. Nerve conduits are seen as promising alternatives to autologous nerve grafts, but their effectiveness is limited by the controlled delivery of bioactive factors and meeting various functional requirements during different stages of repair. This research developed multifunctional nerve conduits using electrospinning and polydopamine (PDA) coating techniques to integrate bioactive substances. Chitosan-composite PLGA electrospun nerve conduits demonstrated exceptional mechanical properties and biocompatibility. Nanofibers with specific topological structures effectively promoted oriented cell growth. The PDA coating provided ROS scavenging and immune modulation functions. The bFGF growth factor attached to the PDA coating facilitated sustained release, enhancing Schwann cell functionality and stimulating neurite outgrowth. In a rat sciatic nerve defect model with a 10 mm gap, PLGA/CS-PDA-bFGF nerve conduits showed a positive impact on nerve regeneration and functional recovery. Consequently, nerve conduits with multiple functions modified with PDA-coated bioactive molecules are poised to be excellent materials for mending peripheral nerve injuries. |
| format | Article |
| id | doaj-art-8998d6e6c2684f7a8b79c947d2d35a46 |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-8998d6e6c2684f7a8b79c947d2d35a462025-02-12T05:31:42ZengElsevierMaterials Today Bio2590-00642025-04-0131101543An immunoregulation PLGA/Chitosan aligned nanofibers with polydopamine coupling basic fibroblast growth factor and ROS scavenging for peripheral nerve regenerationXiaokun Chen0Jihai Xu1Feng Qin2Ziyuan Yang3Xueyuan Li4Miao Yu5Ming Li6Yanhua Wang7Wang Xin8Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, ChinaDepartment of Hand Surgery, Department of Plastic Reconstructive Surgery, Ningbo No.6 Hospital, Ningbo, 315040, ChinaKey Laboratory of Trauma and Neural Regeneration, Ministry of Education, Peking University, Beijing, 100044, China; National Center for Trauma Medicine, Beijing, 100044, China; Department of Orthopedics and Trauma, Peking University People's Hospital, Beijing, 100044, ChinaKey Laboratory of Trauma and Neural Regeneration, Ministry of Education, Peking University, Beijing, 100044, China; National Center for Trauma Medicine, Beijing, 100044, China; Trauma Medicine Center, Peking University People's Hospital, Beijing, 100044, ChinaDepartment of Hand Surgery, Ningbo No.6 Hospital, Ningbo, 315040, ChinaDepartment of Hand Surgery, Ningbo No.6 Hospital, Ningbo, 315040, ChinaKey Laboratory of Trauma and Neural Regeneration, Ministry of Education, Peking University, Beijing, 100044, China; National Center for Trauma Medicine, Beijing, 100044, China; Trauma Medicine Center, Peking University People's Hospital, Beijing, 100044, China; Corresponding author. Key Laboratory of Trauma and Neural Regeneration, Ministry of Education, Peking University, Beijing, 100044, China.Key Laboratory of Trauma and Neural Regeneration, Ministry of Education, Peking University, Beijing, 100044, China; National Center for Trauma Medicine, Beijing, 100044, China; Department of Orthopedics and Trauma, Peking University People's Hospital, Beijing, 100044, China; Corresponding author. Key Laboratory of Trauma and Neural Regeneration, Ministry of Education, Peking University, Beijing, 100044, China.Department of Plastic Reconstructive Surgery, Ningbo No.6 Hospital, Ningbo, 315040, China; Corresponding author.The repair and functional recovery of long-segment peripheral nerve injuries are crucial in clinical settings. Nerve conduits are seen as promising alternatives to autologous nerve grafts, but their effectiveness is limited by the controlled delivery of bioactive factors and meeting various functional requirements during different stages of repair. This research developed multifunctional nerve conduits using electrospinning and polydopamine (PDA) coating techniques to integrate bioactive substances. Chitosan-composite PLGA electrospun nerve conduits demonstrated exceptional mechanical properties and biocompatibility. Nanofibers with specific topological structures effectively promoted oriented cell growth. The PDA coating provided ROS scavenging and immune modulation functions. The bFGF growth factor attached to the PDA coating facilitated sustained release, enhancing Schwann cell functionality and stimulating neurite outgrowth. In a rat sciatic nerve defect model with a 10 mm gap, PLGA/CS-PDA-bFGF nerve conduits showed a positive impact on nerve regeneration and functional recovery. Consequently, nerve conduits with multiple functions modified with PDA-coated bioactive molecules are poised to be excellent materials for mending peripheral nerve injuries.http://www.sciencedirect.com/science/article/pii/S2590006425001012Composite nerve conduitsElectrospun nanofibersPolydopamineImmune modulationPeripheral nerve regeneration |
| spellingShingle | Xiaokun Chen Jihai Xu Feng Qin Ziyuan Yang Xueyuan Li Miao Yu Ming Li Yanhua Wang Wang Xin An immunoregulation PLGA/Chitosan aligned nanofibers with polydopamine coupling basic fibroblast growth factor and ROS scavenging for peripheral nerve regeneration Materials Today Bio Composite nerve conduits Electrospun nanofibers Polydopamine Immune modulation Peripheral nerve regeneration |
| title | An immunoregulation PLGA/Chitosan aligned nanofibers with polydopamine coupling basic fibroblast growth factor and ROS scavenging for peripheral nerve regeneration |
| title_full | An immunoregulation PLGA/Chitosan aligned nanofibers with polydopamine coupling basic fibroblast growth factor and ROS scavenging for peripheral nerve regeneration |
| title_fullStr | An immunoregulation PLGA/Chitosan aligned nanofibers with polydopamine coupling basic fibroblast growth factor and ROS scavenging for peripheral nerve regeneration |
| title_full_unstemmed | An immunoregulation PLGA/Chitosan aligned nanofibers with polydopamine coupling basic fibroblast growth factor and ROS scavenging for peripheral nerve regeneration |
| title_short | An immunoregulation PLGA/Chitosan aligned nanofibers with polydopamine coupling basic fibroblast growth factor and ROS scavenging for peripheral nerve regeneration |
| title_sort | immunoregulation plga chitosan aligned nanofibers with polydopamine coupling basic fibroblast growth factor and ros scavenging for peripheral nerve regeneration |
| topic | Composite nerve conduits Electrospun nanofibers Polydopamine Immune modulation Peripheral nerve regeneration |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425001012 |
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