GC/MS analysis and potential synergistic effect of mandarin and marjoram oils on Helicobacter pylori
Helicobacter pylori can cause chronic gastritis, peptic ulcer, and gastric carcinoma. This study compares chemical composition and anti-H. pylori activity of mandarin leaves and marjoram herb essential oils, and their combined oil. GC/MS analysis of mandarin oil revealed six compounds (100% identifi...
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Taylor & Francis Group
2022-12-01
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| Series: | Journal of Enzyme Inhibition and Medicinal Chemistry |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/14756366.2022.2081846 |
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| author | Rawah H. Elkousy Nada M. Mostafa Ahmed M. Abd-alkhalek Mahmoud A. El Hassab Sara T. Al-Rashood Wagdy M. Eldehna Omayma A. Eldahshan |
| author_facet | Rawah H. Elkousy Nada M. Mostafa Ahmed M. Abd-alkhalek Mahmoud A. El Hassab Sara T. Al-Rashood Wagdy M. Eldehna Omayma A. Eldahshan |
| author_sort | Rawah H. Elkousy |
| collection | DOAJ |
| description | Helicobacter pylori can cause chronic gastritis, peptic ulcer, and gastric carcinoma. This study compares chemical composition and anti-H. pylori activity of mandarin leaves and marjoram herb essential oils, and their combined oil. GC/MS analysis of mandarin oil revealed six compounds (100% identified), mainly methyl-N-methyl anthranilate (89.93%), and 13 compounds (93.52% identified) of marjoram oil, mainly trans-sabinene hydrate (36.11%), terpinen-4-ol (17.97%), linalyl acetate (9.18%), and caryophyllene oxide (8.25%)). Marjoram oil (MIC = 11.40 µg/mL) demonstrated higher activity than mandarin oil (MIC = 31.25 µg/mL). The combined oil showed a synergistic effect at MIC of 1.95 µg/mL (same as clarithromycin). In-silico molecular docking on H. pylori urease, CagA, pharmacokinetic and toxicity studies were performed on major compounds from both oils. The best scores were for caryophyllene oxide then linalyl acetate and methyl-N-methyl anthranilate. Compounds revealed high safety and desirable properties. The combined oil can be an excellent candidate to manage H. pylori. |
| format | Article |
| id | doaj-art-8997f6e37a9945349d89150f7e598bba |
| institution | OA Journals |
| issn | 1475-6366 1475-6374 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Enzyme Inhibition and Medicinal Chemistry |
| spelling | doaj-art-8997f6e37a9945349d89150f7e598bba2025-08-20T02:10:17ZengTaylor & Francis GroupJournal of Enzyme Inhibition and Medicinal Chemistry1475-63661475-63742022-12-013711610161910.1080/14756366.2022.2081846GC/MS analysis and potential synergistic effect of mandarin and marjoram oils on Helicobacter pyloriRawah H. Elkousy0Nada M. Mostafa1Ahmed M. Abd-alkhalek2Mahmoud A. El Hassab3Sara T. Al-Rashood4Wagdy M. Eldehna5Omayma A. Eldahshan6Department of Pharmacognosy, Faculty of Pharmacy (for Girls), Al-Azhar University, Cairo, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Cairo, EgyptFaculty of Medicine (for Boys), Al Azhar University, Cairo, EgyptDepartment of Medicinal Chemistry, Faculty of Pharmacy, King Salman International University (KSIU), Ras Sedr, EgyptDepartment of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaSchool of Biotechnology, Badr University in Cairo, Badr City, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Cairo, EgyptHelicobacter pylori can cause chronic gastritis, peptic ulcer, and gastric carcinoma. This study compares chemical composition and anti-H. pylori activity of mandarin leaves and marjoram herb essential oils, and their combined oil. GC/MS analysis of mandarin oil revealed six compounds (100% identified), mainly methyl-N-methyl anthranilate (89.93%), and 13 compounds (93.52% identified) of marjoram oil, mainly trans-sabinene hydrate (36.11%), terpinen-4-ol (17.97%), linalyl acetate (9.18%), and caryophyllene oxide (8.25%)). Marjoram oil (MIC = 11.40 µg/mL) demonstrated higher activity than mandarin oil (MIC = 31.25 µg/mL). The combined oil showed a synergistic effect at MIC of 1.95 µg/mL (same as clarithromycin). In-silico molecular docking on H. pylori urease, CagA, pharmacokinetic and toxicity studies were performed on major compounds from both oils. The best scores were for caryophyllene oxide then linalyl acetate and methyl-N-methyl anthranilate. Compounds revealed high safety and desirable properties. The combined oil can be an excellent candidate to manage H. pylori.https://www.tandfonline.com/doi/10.1080/14756366.2022.2081846Marjoram oilpetitgrain mandarin oilHelicobacter pyloriessential oilsclarithromycin |
| spellingShingle | Rawah H. Elkousy Nada M. Mostafa Ahmed M. Abd-alkhalek Mahmoud A. El Hassab Sara T. Al-Rashood Wagdy M. Eldehna Omayma A. Eldahshan GC/MS analysis and potential synergistic effect of mandarin and marjoram oils on Helicobacter pylori Journal of Enzyme Inhibition and Medicinal Chemistry Marjoram oil petitgrain mandarin oil Helicobacter pylori essential oils clarithromycin |
| title | GC/MS analysis and potential synergistic effect of mandarin and marjoram oils on Helicobacter pylori |
| title_full | GC/MS analysis and potential synergistic effect of mandarin and marjoram oils on Helicobacter pylori |
| title_fullStr | GC/MS analysis and potential synergistic effect of mandarin and marjoram oils on Helicobacter pylori |
| title_full_unstemmed | GC/MS analysis and potential synergistic effect of mandarin and marjoram oils on Helicobacter pylori |
| title_short | GC/MS analysis and potential synergistic effect of mandarin and marjoram oils on Helicobacter pylori |
| title_sort | gc ms analysis and potential synergistic effect of mandarin and marjoram oils on helicobacter pylori |
| topic | Marjoram oil petitgrain mandarin oil Helicobacter pylori essential oils clarithromycin |
| url | https://www.tandfonline.com/doi/10.1080/14756366.2022.2081846 |
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