A callus transformation system for gene functional studies in soybean
Obtaining transgenic plants is a common method for analyzing gene function. Unfortunately, stable genetic transformation is difficult to achieve, especially for plants (e.g., soybean), which are recalcitrant to genetic transformation. Transient expression systems, such as Arabidopsis protoplast, Nic...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2017-09-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311916616210 |
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| author | Kun XU Xiao-mei ZHANG Cheng-ming FAN Fu-lu CHEN Jin-long ZHU Shi-long ZHANG Qing-shan CHEN Yong-fu FU |
| author_facet | Kun XU Xiao-mei ZHANG Cheng-ming FAN Fu-lu CHEN Jin-long ZHU Shi-long ZHANG Qing-shan CHEN Yong-fu FU |
| author_sort | Kun XU |
| collection | DOAJ |
| description | Obtaining transgenic plants is a common method for analyzing gene function. Unfortunately, stable genetic transformation is difficult to achieve, especially for plants (e.g., soybean), which are recalcitrant to genetic transformation. Transient expression systems, such as Arabidopsis protoplast, Nicotiana leaves, and onion bulb leaves are widely used for gene functional studies. A simple method for obtaining transgenic soybean callus tissues was reported recently. We extend this system with simplified culture conditions to gene functional studies, including promoter analysis, expression and subcellular localization of the target protein, and protein-protein interaction. We also evaluate the plasticity of this system with soybean varieties, different vector constructs, and various Agrobacterium strains. The results indicated that the callus transformation system is efficient and adaptable for gene functional investigation in soybean genotype-, vector-, and Agrobacterium strain-independent modes. We demonstrated an easy set-up and practical homologous strategy for soybean gene functional studies. |
| format | Article |
| id | doaj-art-89904d3a007a419d9976a0673ec0b8ac |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2017-09-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-89904d3a007a419d9976a0673ec0b8ac2025-08-20T03:56:58ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192017-09-011691913192210.1016/S2095-3119(16)61621-0A callus transformation system for gene functional studies in soybeanKun XU0Xiao-mei ZHANG1Cheng-ming FAN2Fu-lu CHEN3Jin-long ZHU4Shi-long ZHANG5Qing-shan CHEN6Yong-fu FU7Key Lab of Soybean Biology (Beijing), Ministry of Agriculture/National Key Facility of Crop Gene Resource and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.ChinaKey Lab of Soybean Biology (Beijing), Ministry of Agriculture/National Key Facility of Crop Gene Resource and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.ChinaKey Lab of Soybean Biology (Beijing), Ministry of Agriculture/National Key Facility of Crop Gene Resource and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.ChinaKey Lab of Soybean Biology (Beijing), Ministry of Agriculture/National Key Facility of Crop Gene Resource and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.ChinaKey Lab of Soybean Biology (Beijing), Ministry of Agriculture/National Key Facility of Crop Gene Resource and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.ChinaBijie Agricultural Science Research Institute of Guizhou, Bijie 551700, P.R.ChinaCollege of Agriculture, Northeast Agricultural University, Harbin 150030, P.R.ChinaKey Lab of Soybean Biology (Beijing), Ministry of Agriculture/National Key Facility of Crop Gene Resource and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China; Correspondence FU Yong-fu, Tel/Fax: +86-10-82105864Obtaining transgenic plants is a common method for analyzing gene function. Unfortunately, stable genetic transformation is difficult to achieve, especially for plants (e.g., soybean), which are recalcitrant to genetic transformation. Transient expression systems, such as Arabidopsis protoplast, Nicotiana leaves, and onion bulb leaves are widely used for gene functional studies. A simple method for obtaining transgenic soybean callus tissues was reported recently. We extend this system with simplified culture conditions to gene functional studies, including promoter analysis, expression and subcellular localization of the target protein, and protein-protein interaction. We also evaluate the plasticity of this system with soybean varieties, different vector constructs, and various Agrobacterium strains. The results indicated that the callus transformation system is efficient and adaptable for gene functional investigation in soybean genotype-, vector-, and Agrobacterium strain-independent modes. We demonstrated an easy set-up and practical homologous strategy for soybean gene functional studies.http://www.sciencedirect.com/science/article/pii/S2095311916616210soybean callusgene function studiestransformation |
| spellingShingle | Kun XU Xiao-mei ZHANG Cheng-ming FAN Fu-lu CHEN Jin-long ZHU Shi-long ZHANG Qing-shan CHEN Yong-fu FU A callus transformation system for gene functional studies in soybean Journal of Integrative Agriculture soybean callus gene function studies transformation |
| title | A callus transformation system for gene functional studies in soybean |
| title_full | A callus transformation system for gene functional studies in soybean |
| title_fullStr | A callus transformation system for gene functional studies in soybean |
| title_full_unstemmed | A callus transformation system for gene functional studies in soybean |
| title_short | A callus transformation system for gene functional studies in soybean |
| title_sort | callus transformation system for gene functional studies in soybean |
| topic | soybean callus gene function studies transformation |
| url | http://www.sciencedirect.com/science/article/pii/S2095311916616210 |
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