Monitoring of BLAD, DUMPS, CVM, BC and FXID in Turkish Native Cattle Breeds
In this study, Turkish native cattle breeds were monitored with respect to the genetic disorders defined as bovine leukocyte adhesion deficiency (BLAD), deficiency of uridine monophosphate synthase (DUMPS), complex vertebral malformation (CVM), bovine citrullinaemia (BC) and factor XI deficiency (FX...
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Ankara University
2012-09-01
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| Series: | Journal of Agricultural Sciences |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/1543481 |
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| author | Hasan Meydan |
| author_facet | Hasan Meydan |
| author_sort | Hasan Meydan |
| collection | DOAJ |
| description | In this study, Turkish native cattle breeds were monitored with respect to the genetic disorders defined as bovine leukocyte adhesion deficiency (BLAD), deficiency of uridine monophosphate synthase (DUMPS), complex vertebral malformation (CVM), bovine citrullinaemia (BC) and factor XI deficiency (FXID). All these are autosomal recessive hereditary disorders causing serious economic losses in dairy cattle breeding throughout the world. In order to determine the presence or the absence of BLAD, DUMPS, CVM, BC and FXID genotypes in native cattle breeds, 200 heads of Anatolian Black, 100 heads of East Anatolian Red, 100 heads of Turkish Grey, 50 heads of Anatolian Southern Yellow, 50 heads of South Anatolian Red and 9 heads of Zavot breed (totally 509 heads) were sampled. Genomic DNA was obtained from blood and the amplicons were obtained by using PCR. PCR products were digested with TaqI, AvaI, EcoT22I and AvaII restriction enzymes for BLAD, DUMPS, CVM, and BC, respectively. Digested products of BLAD, DUMPS CVM and BC were analyzed by agarose gel electrophoresis. PCR products of FXID were analyzed by only agarose gel electrophoresis stained with ethidium bromide. The results demonstrated that no animals examined were the carrier of these genetic disorders. Thus, it can be concluded that mutant alleles of BLAD, DUMPS, CVM, BC and FXID are absent in Turkish native cattle breeds. |
| format | Article |
| id | doaj-art-898bdfec35c04d2b8f4142268084b22f |
| institution | OA Journals |
| issn | 1300-7580 2148-9297 |
| language | English |
| publishDate | 2012-09-01 |
| publisher | Ankara University |
| record_format | Article |
| series | Journal of Agricultural Sciences |
| spelling | doaj-art-898bdfec35c04d2b8f4142268084b22f2025-08-20T01:58:12ZengAnkara UniversityJournal of Agricultural Sciences1300-75802148-92972012-09-0118323924510.1501/Tarimbil_000000121145Monitoring of BLAD, DUMPS, CVM, BC and FXID in Turkish Native Cattle BreedsHasan MeydanIn this study, Turkish native cattle breeds were monitored with respect to the genetic disorders defined as bovine leukocyte adhesion deficiency (BLAD), deficiency of uridine monophosphate synthase (DUMPS), complex vertebral malformation (CVM), bovine citrullinaemia (BC) and factor XI deficiency (FXID). All these are autosomal recessive hereditary disorders causing serious economic losses in dairy cattle breeding throughout the world. In order to determine the presence or the absence of BLAD, DUMPS, CVM, BC and FXID genotypes in native cattle breeds, 200 heads of Anatolian Black, 100 heads of East Anatolian Red, 100 heads of Turkish Grey, 50 heads of Anatolian Southern Yellow, 50 heads of South Anatolian Red and 9 heads of Zavot breed (totally 509 heads) were sampled. Genomic DNA was obtained from blood and the amplicons were obtained by using PCR. PCR products were digested with TaqI, AvaI, EcoT22I and AvaII restriction enzymes for BLAD, DUMPS, CVM, and BC, respectively. Digested products of BLAD, DUMPS CVM and BC were analyzed by agarose gel electrophoresis. PCR products of FXID were analyzed by only agarose gel electrophoresis stained with ethidium bromide. The results demonstrated that no animals examined were the carrier of these genetic disorders. Thus, it can be concluded that mutant alleles of BLAD, DUMPS, CVM, BC and FXID are absent in Turkish native cattle breeds.https://dergipark.org.tr/tr/download/article-file/1543481bladdumpscvmbcfxidpcr-rflpbladdumpscvmbcfxidpcr-rflp |
| spellingShingle | Hasan Meydan Monitoring of BLAD, DUMPS, CVM, BC and FXID in Turkish Native Cattle Breeds Journal of Agricultural Sciences blad dumps cvm bc fxid pcr-rflp blad dumps cvm bc fxid pcr-rflp |
| title | Monitoring of BLAD, DUMPS, CVM, BC and FXID in Turkish Native Cattle Breeds |
| title_full | Monitoring of BLAD, DUMPS, CVM, BC and FXID in Turkish Native Cattle Breeds |
| title_fullStr | Monitoring of BLAD, DUMPS, CVM, BC and FXID in Turkish Native Cattle Breeds |
| title_full_unstemmed | Monitoring of BLAD, DUMPS, CVM, BC and FXID in Turkish Native Cattle Breeds |
| title_short | Monitoring of BLAD, DUMPS, CVM, BC and FXID in Turkish Native Cattle Breeds |
| title_sort | monitoring of blad dumps cvm bc and fxid in turkish native cattle breeds |
| topic | blad dumps cvm bc fxid pcr-rflp blad dumps cvm bc fxid pcr-rflp |
| url | https://dergipark.org.tr/tr/download/article-file/1543481 |
| work_keys_str_mv | AT hasanmeydan monitoringofbladdumpscvmbcandfxidinturkishnativecattlebreeds |