Inhibitory effect of rutin on the growth and metastasis of osteosarcoma in vitro and in vivo
Objective·To investigate the effects of rutin on proliferation, apoptosis, migration and invasion of osteosarcoma cells and its possible molecular mechanisms.Methods·Human osteosarcoma MG63 and U2OS cells were treated with rutin at concentrations of 10, 20 and 40 μmol/L, respectively. The effects of...
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Editorial Office of Journal of Shanghai Jiao Tong University (Medical Science)
2025-01-01
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| Series: | Shanghai Jiaotong Daxue xuebao. Yixue ban |
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| Online Access: | https://xuebao.shsmu.edu.cn/article/2025/1674-8115/1674-8115-2025-45-1-20.shtml |
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| author | LI Xiang WEI Ming WU Wenxi LUO Xiaoqin YAO Biao WU Siyu |
| author_facet | LI Xiang WEI Ming WU Wenxi LUO Xiaoqin YAO Biao WU Siyu |
| author_sort | LI Xiang |
| collection | DOAJ |
| description | Objective·To investigate the effects of rutin on proliferation, apoptosis, migration and invasion of osteosarcoma cells and its possible molecular mechanisms.Methods·Human osteosarcoma MG63 and U2OS cells were treated with rutin at concentrations of 10, 20 and 40 μmol/L, respectively. The effects of rutin on proliferation, apoptosis, migration, and invasion of MG63 and U2OS cells were assessed by using CCK-8 assay, colony formation assay, flow cytometry, scratch closure assay, and Transwell assay. The expression levels of cell proliferation antigen Ki67, B-cell lymphoma-2 (Bcl-2), and Bcl-2 associated X protein (Bax) proteins were detected by Western blotting. Twelve BALB/c nude mice were subcutaneously injected with osteosarcoma MG63 cells to establish a subcutaneous transplant tumor model. The mice were randomly divided into two groups: a control group and a rutin 40 mg/kg group (6 mice in each group). The rutin 40 mg/kg group was intraperitoneally injected with rutin (40 mg/kg), and the control group was intraperitoneally injected with an equal volume of saline, once every other day for 4 weeks. The tumor volume was measured every week. After 4 weeks, the mice were euthanized, and the tumors were excised and weighed. Immunohistochemistry was used to detect the expression of Ki67 and vascular endothelial growth factor (VEGF) in tumor tissues. TUNEL was used to detect tumor cell apoptosis.Results·Compared with MG63 and U2OS cells not treated with rutin, MG63 and U2OS cells treated with rutin at 20 and 40 μmol/L showed a significant decrease in proliferation rate, an increase in apoptotic rate, a decrease in migration and invasion abilities, a significant downregulation of Ki67 protein, and a significant increase in Bax/Bcl-2 ratio, with statistically significant differences (all P<0.05). In addition, rutin significantly inhibited the in vivo growth of osteosarcoma cells, reduced the expression of Ki67 and VEGF in tumor tissues, and promoted cell apoptosis (all P<0.05).Conclusion·Rutin can inhibit the proliferation, migration, and invasion of osteosarcoma cells, and promote apoptosis. |
| format | Article |
| id | doaj-art-897bee9ed4b447e38dcd3e7f642549e8 |
| institution | DOAJ |
| issn | 1674-8115 |
| language | zho |
| publishDate | 2025-01-01 |
| publisher | Editorial Office of Journal of Shanghai Jiao Tong University (Medical Science) |
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| series | Shanghai Jiaotong Daxue xuebao. Yixue ban |
| spelling | doaj-art-897bee9ed4b447e38dcd3e7f642549e82025-08-20T03:13:03ZzhoEditorial Office of Journal of Shanghai Jiao Tong University (Medical Science)Shanghai Jiaotong Daxue xuebao. Yixue ban1674-81152025-01-01451202810.3969/j.issn.1674-8115.2025.01.0031674-8115（2025）01-0020-09Inhibitory effect of rutin on the growth and metastasis of osteosarcoma in vitro and in vivoLI Xiang0WEI Ming1WU Wenxi2LUO Xiaoqin3YAO Biao4WU Siyu5Department of Emergency Medicine, Sichuan Taikang Hospital, Chengdu 610213, ChinaDepartment of Emergency Medicine, Sichuan Taikang Hospital, Chengdu 610213, ChinaDepartment of Emergency Medicine, Sichuan Taikang Hospital, Chengdu 610213, ChinaDepartment of Endocrinology, Chengfei General Medical Hospital, Chengdu 610091, ChinaDepartment of Emergency, Chengdu Eastern District Hospital, Sichuan Province, Chengdu 610023, ChinaDepartment of Emergency Medicine, Sichuan Taikang Hospital, Chengdu 610213, ChinaObjective·To investigate the effects of rutin on proliferation, apoptosis, migration and invasion of osteosarcoma cells and its possible molecular mechanisms.Methods·Human osteosarcoma MG63 and U2OS cells were treated with rutin at concentrations of 10, 20 and 40 μmol/L, respectively. The effects of rutin on proliferation, apoptosis, migration, and invasion of MG63 and U2OS cells were assessed by using CCK-8 assay, colony formation assay, flow cytometry, scratch closure assay, and Transwell assay. The expression levels of cell proliferation antigen Ki67, B-cell lymphoma-2 (Bcl-2), and Bcl-2 associated X protein (Bax) proteins were detected by Western blotting. Twelve BALB/c nude mice were subcutaneously injected with osteosarcoma MG63 cells to establish a subcutaneous transplant tumor model. The mice were randomly divided into two groups: a control group and a rutin 40 mg/kg group (6 mice in each group). The rutin 40 mg/kg group was intraperitoneally injected with rutin (40 mg/kg), and the control group was intraperitoneally injected with an equal volume of saline, once every other day for 4 weeks. The tumor volume was measured every week. After 4 weeks, the mice were euthanized, and the tumors were excised and weighed. Immunohistochemistry was used to detect the expression of Ki67 and vascular endothelial growth factor (VEGF) in tumor tissues. TUNEL was used to detect tumor cell apoptosis.Results·Compared with MG63 and U2OS cells not treated with rutin, MG63 and U2OS cells treated with rutin at 20 and 40 μmol/L showed a significant decrease in proliferation rate, an increase in apoptotic rate, a decrease in migration and invasion abilities, a significant downregulation of Ki67 protein, and a significant increase in Bax/Bcl-2 ratio, with statistically significant differences (all P<0.05). In addition, rutin significantly inhibited the in vivo growth of osteosarcoma cells, reduced the expression of Ki67 and VEGF in tumor tissues, and promoted cell apoptosis (all P<0.05).Conclusion·Rutin can inhibit the proliferation, migration, and invasion of osteosarcoma cells, and promote apoptosis.https://xuebao.shsmu.edu.cn/article/2025/1674-8115/1674-8115-2025-45-1-20.shtmlrutinosteosarcomaproliferationinvasioncell proliferation antigen ki67 |
| spellingShingle | LI Xiang WEI Ming WU Wenxi LUO Xiaoqin YAO Biao WU Siyu Inhibitory effect of rutin on the growth and metastasis of osteosarcoma in vitro and in vivo Shanghai Jiaotong Daxue xuebao. Yixue ban rutin osteosarcoma proliferation invasion cell proliferation antigen ki67 |
| title | Inhibitory effect of rutin on the growth and metastasis of osteosarcoma in vitro and in vivo |
| title_full | Inhibitory effect of rutin on the growth and metastasis of osteosarcoma in vitro and in vivo |
| title_fullStr | Inhibitory effect of rutin on the growth and metastasis of osteosarcoma in vitro and in vivo |
| title_full_unstemmed | Inhibitory effect of rutin on the growth and metastasis of osteosarcoma in vitro and in vivo |
| title_short | Inhibitory effect of rutin on the growth and metastasis of osteosarcoma in vitro and in vivo |
| title_sort | inhibitory effect of rutin on the growth and metastasis of osteosarcoma in vitro and in vivo |
| topic | rutin osteosarcoma proliferation invasion cell proliferation antigen ki67 |
| url | https://xuebao.shsmu.edu.cn/article/2025/1674-8115/1674-8115-2025-45-1-20.shtml |
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