Determination of Real-Time Polymerase Chain Reaction Uncertainty of Measurement Using Replicate Analysis and a Graphical User Interface with Fieller’s Theorem
BACKGROUND: Disease monitoring of viruses using real-time polymerase chain reaction (PCR) requires knowledge of the precision of the test to determine what constitutes a significant change. Calculation of quantitative PCR confidence limits requires bivariate statistical methods.
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2014/404517 |
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| _version_ | 1832547022432894976 |
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| author | James Ian Stuart Johan Delport Robert Lannigan George Zahariadis |
| author_facet | James Ian Stuart Johan Delport Robert Lannigan George Zahariadis |
| author_sort | James Ian Stuart |
| collection | DOAJ |
| description | BACKGROUND: Disease monitoring of viruses using real-time polymerase chain reaction (PCR) requires knowledge of the precision of the test to determine what constitutes a significant change. Calculation of quantitative PCR confidence limits requires bivariate statistical methods. |
| format | Article |
| id | doaj-art-897ba5cee2ab40d2b3c0b0e0e4451169 |
| institution | Kabale University |
| issn | 1712-9532 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-897ba5cee2ab40d2b3c0b0e0e44511692025-02-03T06:46:23ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95322014-01-0125420721010.1155/2014/404517Determination of Real-Time Polymerase Chain Reaction Uncertainty of Measurement Using Replicate Analysis and a Graphical User Interface with Fieller’s TheoremJames Ian Stuart0Johan Delport1Robert Lannigan2George Zahariadis3London Health Sciences Centre and Western University, London, Ontario, CanadaLondon Health Sciences Centre and Western University, London, Ontario, CanadaLondon Health Sciences Centre and Western University, London, Ontario, CanadaLondon Health Sciences Centre and Western University, London, Ontario, CanadaBACKGROUND: Disease monitoring of viruses using real-time polymerase chain reaction (PCR) requires knowledge of the precision of the test to determine what constitutes a significant change. Calculation of quantitative PCR confidence limits requires bivariate statistical methods.http://dx.doi.org/10.1155/2014/404517 |
| spellingShingle | James Ian Stuart Johan Delport Robert Lannigan George Zahariadis Determination of Real-Time Polymerase Chain Reaction Uncertainty of Measurement Using Replicate Analysis and a Graphical User Interface with Fieller’s Theorem Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Determination of Real-Time Polymerase Chain Reaction Uncertainty of Measurement Using Replicate Analysis and a Graphical User Interface with Fieller’s Theorem |
| title_full | Determination of Real-Time Polymerase Chain Reaction Uncertainty of Measurement Using Replicate Analysis and a Graphical User Interface with Fieller’s Theorem |
| title_fullStr | Determination of Real-Time Polymerase Chain Reaction Uncertainty of Measurement Using Replicate Analysis and a Graphical User Interface with Fieller’s Theorem |
| title_full_unstemmed | Determination of Real-Time Polymerase Chain Reaction Uncertainty of Measurement Using Replicate Analysis and a Graphical User Interface with Fieller’s Theorem |
| title_short | Determination of Real-Time Polymerase Chain Reaction Uncertainty of Measurement Using Replicate Analysis and a Graphical User Interface with Fieller’s Theorem |
| title_sort | determination of real time polymerase chain reaction uncertainty of measurement using replicate analysis and a graphical user interface with fieller s theorem |
| url | http://dx.doi.org/10.1155/2014/404517 |
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