Identification and characterization of native arbuscular mycorrhizal fungi in plants growing under organic and conventional farming conditions in Oman
Although arbuscular mycorrhizal fungi (AMF) are widespread in both natural and agricultural environments, their biodiversity and community structure are not well understood. This is particularly true for the influences of different farming practices on their taxonomic distribution. This study aimed...
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Elsevier
2025-04-01
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| Series: | Soil & Environmental Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2949919425000135 |
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| author | Ali Al-Hinai Rhonda Janke Ewald Sieverding Muhammad Farooq Daniel Menezes-Blackburn |
| author_facet | Ali Al-Hinai Rhonda Janke Ewald Sieverding Muhammad Farooq Daniel Menezes-Blackburn |
| author_sort | Ali Al-Hinai |
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| description | Although arbuscular mycorrhizal fungi (AMF) are widespread in both natural and agricultural environments, their biodiversity and community structure are not well understood. This is particularly true for the influences of different farming practices on their taxonomic distribution. This study aimed to investigate the influence of organic and conventional farming on AMF diversity in the rhizosphere soils of three crops includng mango, citrus and cucumber from Oman. Spore morphological analysis and internal transcribed spacer (ITS) sequencing were used to identify AMF species diversity. Spore morphological analysis revealed a total of 9 families, 20 genera, and 36 species, and in general, organic farming samples showed higher diversity than conventional farming samples. The ITS sequencing analysis showed the presence of 6 families and 12 genera, with organic farming samples being significantly more diverse than conventional farming samples based on the number of taxa and Shannon H index. Both spore morphology and ITS sequencing data showed that the families Diversisporaceae and Glomeraceae were the most abundant among all the treatments. Compared to their respective conventional soils, the mean percent mycorrhizal inoculum potential was significantly higher for the organic soils under cucumber, with the root colonization being also higher for organic soils under citrus. A quick colonizer trap culture method was used to determine which AMF species colonized and established in the roots after 14 days. AMF species including Rhizoglomus intraradices and Septoglomus viscosum quickly infected cucumber, and could potentially be used as biofertilizer inocula. The results help to understand the AMF diversity across different farming systems in semiarid and arid regions, as well as to promote and develop sustainable agriculture in these regions. |
| format | Article |
| id | doaj-art-8971ac4b2ab74921ab2f7396501fa1b2 |
| institution | OA Journals |
| issn | 2949-9194 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Soil & Environmental Health |
| spelling | doaj-art-8971ac4b2ab74921ab2f7396501fa1b22025-08-20T02:12:46ZengElsevierSoil & Environmental Health2949-91942025-04-013210014010.1016/j.seh.2025.100140Identification and characterization of native arbuscular mycorrhizal fungi in plants growing under organic and conventional farming conditions in OmanAli Al-Hinai0Rhonda Janke1Ewald Sieverding2Muhammad Farooq3Daniel Menezes-Blackburn4Department of Plant Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Al- Khoud 123, Sultanate of OmanDepartment of Plant Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Al- Khoud 123, Sultanate of OmanInstitute of Agricultural Sciences in the Tropics (Hans-Ruthenberg-Institute), Department for Plant Production and Agroecology in the Tropics and Subtropics, University of Hohenheim, Garbenstrasse 13, D-70599 Stuttgart, GermanyDepartment of Plant Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Al- Khoud 123, Sultanate of OmanDepartment of Soils, Water and Agricultural Engineering, Sultan Qaboos University, PO Box 34, Al-Khoud, 123 Muscat, Sultanate of Oman; Corresponding author.Although arbuscular mycorrhizal fungi (AMF) are widespread in both natural and agricultural environments, their biodiversity and community structure are not well understood. This is particularly true for the influences of different farming practices on their taxonomic distribution. This study aimed to investigate the influence of organic and conventional farming on AMF diversity in the rhizosphere soils of three crops includng mango, citrus and cucumber from Oman. Spore morphological analysis and internal transcribed spacer (ITS) sequencing were used to identify AMF species diversity. Spore morphological analysis revealed a total of 9 families, 20 genera, and 36 species, and in general, organic farming samples showed higher diversity than conventional farming samples. The ITS sequencing analysis showed the presence of 6 families and 12 genera, with organic farming samples being significantly more diverse than conventional farming samples based on the number of taxa and Shannon H index. Both spore morphology and ITS sequencing data showed that the families Diversisporaceae and Glomeraceae were the most abundant among all the treatments. Compared to their respective conventional soils, the mean percent mycorrhizal inoculum potential was significantly higher for the organic soils under cucumber, with the root colonization being also higher for organic soils under citrus. A quick colonizer trap culture method was used to determine which AMF species colonized and established in the roots after 14 days. AMF species including Rhizoglomus intraradices and Septoglomus viscosum quickly infected cucumber, and could potentially be used as biofertilizer inocula. The results help to understand the AMF diversity across different farming systems in semiarid and arid regions, as well as to promote and develop sustainable agriculture in these regions.http://www.sciencedirect.com/science/article/pii/S2949919425000135Microbial diversityRhizosphere root zoneFarm soil propertiesAMF species richnessBiofertilizer inoculumSemiarid and arid regions |
| spellingShingle | Ali Al-Hinai Rhonda Janke Ewald Sieverding Muhammad Farooq Daniel Menezes-Blackburn Identification and characterization of native arbuscular mycorrhizal fungi in plants growing under organic and conventional farming conditions in Oman Soil & Environmental Health Microbial diversity Rhizosphere root zone Farm soil properties AMF species richness Biofertilizer inoculum Semiarid and arid regions |
| title | Identification and characterization of native arbuscular mycorrhizal fungi in plants growing under organic and conventional farming conditions in Oman |
| title_full | Identification and characterization of native arbuscular mycorrhizal fungi in plants growing under organic and conventional farming conditions in Oman |
| title_fullStr | Identification and characterization of native arbuscular mycorrhizal fungi in plants growing under organic and conventional farming conditions in Oman |
| title_full_unstemmed | Identification and characterization of native arbuscular mycorrhizal fungi in plants growing under organic and conventional farming conditions in Oman |
| title_short | Identification and characterization of native arbuscular mycorrhizal fungi in plants growing under organic and conventional farming conditions in Oman |
| title_sort | identification and characterization of native arbuscular mycorrhizal fungi in plants growing under organic and conventional farming conditions in oman |
| topic | Microbial diversity Rhizosphere root zone Farm soil properties AMF species richness Biofertilizer inoculum Semiarid and arid regions |
| url | http://www.sciencedirect.com/science/article/pii/S2949919425000135 |
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