Comprehensive microRNA analysis toward exploring a new functional component in Matcha green tea
Matcha, a traditional Japanese green tea, has health-promoting effects. However, little is known about its bioactive components, except for polyphenols, caffeine, and amino acids. Here, we revealed the presence of diverse miRNAs, a type of functional RNAs, as new components of Matcha, using next-gen...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Food Chemistry: Molecular Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666566225000267 |
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| author | Yi-Lan Huang Tomomi Morikawa-Ichinose Seong-Uk Lee Yuka Tatsumi Masaki Ichitani Motofumi Kumazoe Hirofumi Tachibana Yoshinori Fujimura |
| author_facet | Yi-Lan Huang Tomomi Morikawa-Ichinose Seong-Uk Lee Yuka Tatsumi Masaki Ichitani Motofumi Kumazoe Hirofumi Tachibana Yoshinori Fujimura |
| author_sort | Yi-Lan Huang |
| collection | DOAJ |
| description | Matcha, a traditional Japanese green tea, has health-promoting effects. However, little is known about its bioactive components, except for polyphenols, caffeine, and amino acids. Here, we revealed the presence of diverse miRNAs, a type of functional RNAs, as new components of Matcha, using next-generation sequencing. Quantitative reverse-transcription PCR analysis of 10 different Matcha showed that miRNA levels varied depending on the cultivar and harvest season. As extraction methods of miRNAs, we found that soaking at 95 °C significantly enhanced total RNA and miRNA yields. Furthermore, a positive correlation was observed between total RNA and miRNA yields extracted from 27 plant-based dried powders. Notably, Matcha exhibited the highest levels of four representative miRNAs: lja-miR166-3p, csn-miR396d-5p, gma-miR396e, and csn-miRn409. The miRNA yields in Matcha were correlated with the major Matcha components. These results highlight Matcha as a source of miRNAs and candidate bioactive components. These findings provide new insights into the functionality of Matcha. |
| format | Article |
| id | doaj-art-896f2c44eda347e0b2fff80da563b3b1 |
| institution | DOAJ |
| issn | 2666-5662 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: Molecular Sciences |
| spelling | doaj-art-896f2c44eda347e0b2fff80da563b3b12025-08-20T03:21:01ZengElsevierFood Chemistry: Molecular Sciences2666-56622025-06-011010026510.1016/j.fochms.2025.100265Comprehensive microRNA analysis toward exploring a new functional component in Matcha green teaYi-Lan Huang0Tomomi Morikawa-Ichinose1Seong-Uk Lee2Yuka Tatsumi3Masaki Ichitani4Motofumi Kumazoe5Hirofumi Tachibana6Yoshinori Fujimura7Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, JapanDivision of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, JapanDivision of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, JapanCentral Research Institute, ITOEN, Ltd., 21 Mekami, Makinohara-shi, Shizuoka 421-0516, JapanCentral Research Institute, ITOEN, Ltd., 21 Mekami, Makinohara-shi, Shizuoka 421-0516, JapanDivision of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, JapanDivision of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, JapanDivision of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan; Corresponding author.Matcha, a traditional Japanese green tea, has health-promoting effects. However, little is known about its bioactive components, except for polyphenols, caffeine, and amino acids. Here, we revealed the presence of diverse miRNAs, a type of functional RNAs, as new components of Matcha, using next-generation sequencing. Quantitative reverse-transcription PCR analysis of 10 different Matcha showed that miRNA levels varied depending on the cultivar and harvest season. As extraction methods of miRNAs, we found that soaking at 95 °C significantly enhanced total RNA and miRNA yields. Furthermore, a positive correlation was observed between total RNA and miRNA yields extracted from 27 plant-based dried powders. Notably, Matcha exhibited the highest levels of four representative miRNAs: lja-miR166-3p, csn-miR396d-5p, gma-miR396e, and csn-miRn409. The miRNA yields in Matcha were correlated with the major Matcha components. These results highlight Matcha as a source of miRNAs and candidate bioactive components. These findings provide new insights into the functionality of Matcha.http://www.sciencedirect.com/science/article/pii/S2666566225000267Green teaMatchaPlant-based dried powdersMicroRNAChemical compositionExtraction methods |
| spellingShingle | Yi-Lan Huang Tomomi Morikawa-Ichinose Seong-Uk Lee Yuka Tatsumi Masaki Ichitani Motofumi Kumazoe Hirofumi Tachibana Yoshinori Fujimura Comprehensive microRNA analysis toward exploring a new functional component in Matcha green tea Food Chemistry: Molecular Sciences Green tea Matcha Plant-based dried powders MicroRNA Chemical composition Extraction methods |
| title | Comprehensive microRNA analysis toward exploring a new functional component in Matcha green tea |
| title_full | Comprehensive microRNA analysis toward exploring a new functional component in Matcha green tea |
| title_fullStr | Comprehensive microRNA analysis toward exploring a new functional component in Matcha green tea |
| title_full_unstemmed | Comprehensive microRNA analysis toward exploring a new functional component in Matcha green tea |
| title_short | Comprehensive microRNA analysis toward exploring a new functional component in Matcha green tea |
| title_sort | comprehensive microrna analysis toward exploring a new functional component in matcha green tea |
| topic | Green tea Matcha Plant-based dried powders MicroRNA Chemical composition Extraction methods |
| url | http://www.sciencedirect.com/science/article/pii/S2666566225000267 |
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