Enhanced Electrochemiluminescence from Ruthenium-Tagged Immune Complex at Flexible Chains for Sensitive Analysis of Glutamate Decarboxylase Antibody
Herein, a sensitive electrochemiluminescence (ECL) immunosensor is designed by immobilizing ruthenium-tagged immune complexes at flexible poly-ethylene-glycol (PEG) chains on the electrode surface, which offers more freedom for the collision of the ruthenium complex at the electrode during the initi...
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| Format: | Article |
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MDPI AG
2025-01-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/15/1/47 |
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| author | Yuyao Zhang Li Qian Qian Zhang Yu Li Yu Liu Dechen Jiang |
| author_facet | Yuyao Zhang Li Qian Qian Zhang Yu Li Yu Liu Dechen Jiang |
| author_sort | Yuyao Zhang |
| collection | DOAJ |
| description | Herein, a sensitive electrochemiluminescence (ECL) immunosensor is designed by immobilizing ruthenium-tagged immune complexes at flexible poly-ethylene-glycol (PEG) chains on the electrode surface, which offers more freedom for the collision of the ruthenium complex at the electrode during the initial ECL reaction. The electrochemical characterizations confirm the loose structure of the assembled layer with the immune complex, providing an increase in the current and the resultant enhanced ECL emissions. Comparing the sensors with the rigid structure, a 34-fold increase in the maximal ECL emission is recorded when PEG3400 is used as a linker. Using the optimized protocol, the prepared immunosensor exhibits a wide-ranging linear response to the model antibody (glutamate decarboxylase antibody) ranging from 10 pg/mL to 10 ng/mL. The detection limit is almost two orders lower than the value using the classic enzyme-linked immunosorbent assay, which offers a new design to enhance ECL emissions and the resultant analytical performance. |
| format | Article |
| id | doaj-art-896673bce1c346069ba4f9653ca0fd5a |
| institution | Kabale University |
| issn | 2079-6374 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-896673bce1c346069ba4f9653ca0fd5a2025-01-24T13:25:33ZengMDPI AGBiosensors2079-63742025-01-011514710.3390/bios15010047Enhanced Electrochemiluminescence from Ruthenium-Tagged Immune Complex at Flexible Chains for Sensitive Analysis of Glutamate Decarboxylase AntibodyYuyao Zhang0Li Qian1Qian Zhang2Yu Li3Yu Liu4Dechen Jiang5State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210092, ChinaSir Run Run Hospital, Nanjing Medical University, Nanjing 211100, ChinaState Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210092, ChinaState Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210092, ChinaSir Run Run Hospital, Nanjing Medical University, Nanjing 211100, ChinaState Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210092, ChinaHerein, a sensitive electrochemiluminescence (ECL) immunosensor is designed by immobilizing ruthenium-tagged immune complexes at flexible poly-ethylene-glycol (PEG) chains on the electrode surface, which offers more freedom for the collision of the ruthenium complex at the electrode during the initial ECL reaction. The electrochemical characterizations confirm the loose structure of the assembled layer with the immune complex, providing an increase in the current and the resultant enhanced ECL emissions. Comparing the sensors with the rigid structure, a 34-fold increase in the maximal ECL emission is recorded when PEG3400 is used as a linker. Using the optimized protocol, the prepared immunosensor exhibits a wide-ranging linear response to the model antibody (glutamate decarboxylase antibody) ranging from 10 pg/mL to 10 ng/mL. The detection limit is almost two orders lower than the value using the classic enzyme-linked immunosorbent assay, which offers a new design to enhance ECL emissions and the resultant analytical performance.https://www.mdpi.com/2079-6374/15/1/47electrochemiluminescenceflexible PEG chainGADAruthenium compleximmunosensors |
| spellingShingle | Yuyao Zhang Li Qian Qian Zhang Yu Li Yu Liu Dechen Jiang Enhanced Electrochemiluminescence from Ruthenium-Tagged Immune Complex at Flexible Chains for Sensitive Analysis of Glutamate Decarboxylase Antibody Biosensors electrochemiluminescence flexible PEG chain GADA ruthenium complex immunosensors |
| title | Enhanced Electrochemiluminescence from Ruthenium-Tagged Immune Complex at Flexible Chains for Sensitive Analysis of Glutamate Decarboxylase Antibody |
| title_full | Enhanced Electrochemiluminescence from Ruthenium-Tagged Immune Complex at Flexible Chains for Sensitive Analysis of Glutamate Decarboxylase Antibody |
| title_fullStr | Enhanced Electrochemiluminescence from Ruthenium-Tagged Immune Complex at Flexible Chains for Sensitive Analysis of Glutamate Decarboxylase Antibody |
| title_full_unstemmed | Enhanced Electrochemiluminescence from Ruthenium-Tagged Immune Complex at Flexible Chains for Sensitive Analysis of Glutamate Decarboxylase Antibody |
| title_short | Enhanced Electrochemiluminescence from Ruthenium-Tagged Immune Complex at Flexible Chains for Sensitive Analysis of Glutamate Decarboxylase Antibody |
| title_sort | enhanced electrochemiluminescence from ruthenium tagged immune complex at flexible chains for sensitive analysis of glutamate decarboxylase antibody |
| topic | electrochemiluminescence flexible PEG chain GADA ruthenium complex immunosensors |
| url | https://www.mdpi.com/2079-6374/15/1/47 |
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