Phytochemical screening, characterization, and in vitro anti-metastatic effects of the methanolic leaf extract of Momordica cardiospermoides in MDA-MB 321 cells
Introduction: Momordica cardiospermoides represents an inexhaustible source of natural products. We examined the acetone and methanolic extracts of M. cardiospermoides leaves’ phytochemical composition, antioxidant activities, and anti-metastatic properties in MDA-MB-231 cells. Methods: The aluminiu...
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Shahrekord University of Medical Sciences
2024-10-01
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| Series: | Journal of HerbMed Pharmacology |
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| Online Access: | https://herbmedpharmacol.com/PDF/jhp-13-562.pdf |
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| author | Mante Dolly Kgakishe Marole Maria Maluleka Kgomotso Welheminah Poopedi Leseilane Mampuru Bernice Monchusi-Khumalo Vusi Gordon Mbazima |
| author_facet | Mante Dolly Kgakishe Marole Maria Maluleka Kgomotso Welheminah Poopedi Leseilane Mampuru Bernice Monchusi-Khumalo Vusi Gordon Mbazima |
| author_sort | Mante Dolly Kgakishe |
| collection | DOAJ |
| description | Introduction: Momordica cardiospermoides represents an inexhaustible source of natural products. We examined the acetone and methanolic extracts of M. cardiospermoides leaves’ phytochemical composition, antioxidant activities, and anti-metastatic properties in MDA-MB-231 cells. Methods: The aluminium chloride and Folin-Ciocalteu methods were employed to determine the extracts’ phytochemical contents. Assessment of antioxidant activities employed the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The extracts were further characterized with ultra-high-performance liquid chromatography (UHPLC). The cell count and viability kit were utilized to assess viability in HEK-293 and MDA-MB-231 cells. The occurrence of cell death was determined by the annexin V and dead cell assay. The methanolic extract was assessed against cell adhesion and migration using the cell-extracellular matrix and wound healing assays. The effects of the methanolic extract on tissue inhibitors, including metalloproteinase-1 (TIMP-1), matrix metalloproteinases-2 (MMP-2), and -9 (MMP-9) were determined by the human angiogenesis antibody array kit and Western blot analysis. Results: The acetone extract had higher antioxidant activity and total contents than the methanolic extract. UHPLC revealed abundant luteolin, luteoloside, quercetin, stearidonic acid, and salicylamide in the acetone extract, and luteolin, astragalin, and trigonelline in the methanolic extract. The methanolic extract was selectively cytotoxic towards MDA-MB-231 cells while the acetone extract was significantly cytotoxic in both cell lines. Apoptosis was induced by the methanolic extract and the acetone extract induced significant necrosis. The methanolic extract suppressed migration by significantly inhibiting wound closure and inhibited cell adhesion. MMP-2 and MMP-9 were significantly downregulated and TIMP-1 was upregulated. Conclusion: The methanolic extract M. cardiospermoides possesses anti-metastatic activity. |
| format | Article |
| id | doaj-art-896574becb8c4559a53302efc09fe92a |
| institution | OA Journals |
| issn | 2345-5004 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Shahrekord University of Medical Sciences |
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| series | Journal of HerbMed Pharmacology |
| spelling | doaj-art-896574becb8c4559a53302efc09fe92a2025-08-20T02:38:49ZengShahrekord University of Medical SciencesJournal of HerbMed Pharmacology2345-50042024-10-0113456257110.34172/jhp.2024.49380jhp-49380Phytochemical screening, characterization, and in vitro anti-metastatic effects of the methanolic leaf extract of Momordica cardiospermoides in MDA-MB 321 cellsMante Dolly Kgakishe0Marole Maria Maluleka1Kgomotso Welheminah Poopedi2Leseilane Mampuru3Bernice Monchusi-Khumalo4Vusi Gordon Mbazima5Department of Biochemistry, Microbiology and Biotechnology, University of Limpopo, South AfricaDepartment of Chemistry, University of Limpopo, South AfricaDepartment of Biochemistry, Microbiology and Biotechnology, University of Limpopo, South AfricaDepartment of Biochemistry, Microbiology and Biotechnology, University of Limpopo, South AfricaDepartment of Biochemistry, Microbiology and Biotechnology, University of Limpopo, South AfricaDepartment of Biochemistry, Microbiology and Biotechnology, University of Limpopo, South AfricaIntroduction: Momordica cardiospermoides represents an inexhaustible source of natural products. We examined the acetone and methanolic extracts of M. cardiospermoides leaves’ phytochemical composition, antioxidant activities, and anti-metastatic properties in MDA-MB-231 cells. Methods: The aluminium chloride and Folin-Ciocalteu methods were employed to determine the extracts’ phytochemical contents. Assessment of antioxidant activities employed the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The extracts were further characterized with ultra-high-performance liquid chromatography (UHPLC). The cell count and viability kit were utilized to assess viability in HEK-293 and MDA-MB-231 cells. The occurrence of cell death was determined by the annexin V and dead cell assay. The methanolic extract was assessed against cell adhesion and migration using the cell-extracellular matrix and wound healing assays. The effects of the methanolic extract on tissue inhibitors, including metalloproteinase-1 (TIMP-1), matrix metalloproteinases-2 (MMP-2), and -9 (MMP-9) were determined by the human angiogenesis antibody array kit and Western blot analysis. Results: The acetone extract had higher antioxidant activity and total contents than the methanolic extract. UHPLC revealed abundant luteolin, luteoloside, quercetin, stearidonic acid, and salicylamide in the acetone extract, and luteolin, astragalin, and trigonelline in the methanolic extract. The methanolic extract was selectively cytotoxic towards MDA-MB-231 cells while the acetone extract was significantly cytotoxic in both cell lines. Apoptosis was induced by the methanolic extract and the acetone extract induced significant necrosis. The methanolic extract suppressed migration by significantly inhibiting wound closure and inhibited cell adhesion. MMP-2 and MMP-9 were significantly downregulated and TIMP-1 was upregulated. Conclusion: The methanolic extract M. cardiospermoides possesses anti-metastatic activity.https://herbmedpharmacol.com/PDF/jhp-13-562.pdfmomordica cardiospermoidesantioxidant activitymatrix metalloproteinases-2 and -9anti-metastasis |
| spellingShingle | Mante Dolly Kgakishe Marole Maria Maluleka Kgomotso Welheminah Poopedi Leseilane Mampuru Bernice Monchusi-Khumalo Vusi Gordon Mbazima Phytochemical screening, characterization, and in vitro anti-metastatic effects of the methanolic leaf extract of Momordica cardiospermoides in MDA-MB 321 cells Journal of HerbMed Pharmacology momordica cardiospermoides antioxidant activity matrix metalloproteinases-2 and -9 anti-metastasis |
| title | Phytochemical screening, characterization, and in vitro anti-metastatic effects of the methanolic leaf extract of Momordica cardiospermoides in MDA-MB 321 cells |
| title_full | Phytochemical screening, characterization, and in vitro anti-metastatic effects of the methanolic leaf extract of Momordica cardiospermoides in MDA-MB 321 cells |
| title_fullStr | Phytochemical screening, characterization, and in vitro anti-metastatic effects of the methanolic leaf extract of Momordica cardiospermoides in MDA-MB 321 cells |
| title_full_unstemmed | Phytochemical screening, characterization, and in vitro anti-metastatic effects of the methanolic leaf extract of Momordica cardiospermoides in MDA-MB 321 cells |
| title_short | Phytochemical screening, characterization, and in vitro anti-metastatic effects of the methanolic leaf extract of Momordica cardiospermoides in MDA-MB 321 cells |
| title_sort | phytochemical screening characterization and in vitro anti metastatic effects of the methanolic leaf extract of momordica cardiospermoides in mda mb 321 cells |
| topic | momordica cardiospermoides antioxidant activity matrix metalloproteinases-2 and -9 anti-metastasis |
| url | https://herbmedpharmacol.com/PDF/jhp-13-562.pdf |
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