Brochosomes as an antireflective camouflage coating for leafhoppers

In nature, insects face immense predation pressure, where visual cues play a vital role in predators locating them. To counter this threat, insects employ a variety of nano- and microstructures on their cuticular layer to manipulate and interact with light, enhancing antireflective properties and pr...

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Main Authors: Wei Wu, Qianzhuo Mao, Zhuang-Xin Ye, Zhenfeng Liao, Hong-Wei Shan, Jun-Min Li, Chuan-Xi Zhang, Jian-Ping Chen
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2025-05-01
Series:eLife
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Online Access:https://elifesciences.org/articles/99639
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author Wei Wu
Qianzhuo Mao
Zhuang-Xin Ye
Zhenfeng Liao
Hong-Wei Shan
Jun-Min Li
Chuan-Xi Zhang
Jian-Ping Chen
author_facet Wei Wu
Qianzhuo Mao
Zhuang-Xin Ye
Zhenfeng Liao
Hong-Wei Shan
Jun-Min Li
Chuan-Xi Zhang
Jian-Ping Chen
author_sort Wei Wu
collection DOAJ
description In nature, insects face immense predation pressure, where visual cues play a vital role in predators locating them. To counter this threat, insects employ a variety of nano- and microstructures on their cuticular layer to manipulate and interact with light, enhancing antireflective properties and providing camouflage or reducing detectability by predators. Leafhoppers have a unique extracuticular coating called brochosome, yet its antireflective functions and protein composition remain unclear. Our study demonstrates strong antireflective properties of brochosomes, effectively reducing reflectance on the cuticle surface, especially in the ultraviolet spectrum, to improve evasion from visual predators. Furthermore, we identify four novel structural proteins of the brochosome (BSM) for the first time. Inhibiting their synthesis by RNAi alters brochosome morphology, impacting the optical properties of the cuticle surface. Evolutionary origin analysis of BSM suggests that brochosomes likely originated from a process involving duplication–divergence. Our study reveals that leafhoppers employ a unique camouflage strategy by secreting brochosomes as antireflection nanocoatings, enabling them to evade natural predators and contributing to their evolutionary success.
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institution OA Journals
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publisher eLife Sciences Publications Ltd
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series eLife
spelling doaj-art-895dbfa5765c43dd85fe4abc3dcc8f682025-08-20T02:38:18ZengeLife Sciences Publications LtdeLife2050-084X2025-05-011310.7554/eLife.99639Brochosomes as an antireflective camouflage coating for leafhoppersWei Wu0https://orcid.org/0000-0002-5440-345XQianzhuo Mao1Zhuang-Xin Ye2Zhenfeng Liao3Hong-Wei Shan4Jun-Min Li5Chuan-Xi Zhang6Jian-Ping Chen7State Key Laboratory of Agricultural Products Safety, Key Laboratory of Biotechnology in Plant Protection of MARA, Zhejiang Key Laboratory of Green Plant Protection, Institute of Plant Virology, Ningbo University, Ningbo, ChinaState Key Laboratory of Agricultural Products Safety, Key Laboratory of Biotechnology in Plant Protection of MARA, Zhejiang Key Laboratory of Green Plant Protection, Institute of Plant Virology, Ningbo University, Ningbo, ChinaState Key Laboratory of Agricultural Products Safety, Key Laboratory of Biotechnology in Plant Protection of MARA, Zhejiang Key Laboratory of Green Plant Protection, Institute of Plant Virology, Ningbo University, Ningbo, ChinaState Key Laboratory for Sustainable Control of Pest and Disease, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory of Agricultural Products Safety, Key Laboratory of Biotechnology in Plant Protection of MARA, Zhejiang Key Laboratory of Green Plant Protection, Institute of Plant Virology, Ningbo University, Ningbo, ChinaState Key Laboratory of Agricultural Products Safety, Key Laboratory of Biotechnology in Plant Protection of MARA, Zhejiang Key Laboratory of Green Plant Protection, Institute of Plant Virology, Ningbo University, Ningbo, ChinaState Key Laboratory of Agricultural Products Safety, Key Laboratory of Biotechnology in Plant Protection of MARA, Zhejiang Key Laboratory of Green Plant Protection, Institute of Plant Virology, Ningbo University, Ningbo, ChinaState Key Laboratory of Agricultural Products Safety, Key Laboratory of Biotechnology in Plant Protection of MARA, Zhejiang Key Laboratory of Green Plant Protection, Institute of Plant Virology, Ningbo University, Ningbo, ChinaIn nature, insects face immense predation pressure, where visual cues play a vital role in predators locating them. To counter this threat, insects employ a variety of nano- and microstructures on their cuticular layer to manipulate and interact with light, enhancing antireflective properties and providing camouflage or reducing detectability by predators. Leafhoppers have a unique extracuticular coating called brochosome, yet its antireflective functions and protein composition remain unclear. Our study demonstrates strong antireflective properties of brochosomes, effectively reducing reflectance on the cuticle surface, especially in the ultraviolet spectrum, to improve evasion from visual predators. Furthermore, we identify four novel structural proteins of the brochosome (BSM) for the first time. Inhibiting their synthesis by RNAi alters brochosome morphology, impacting the optical properties of the cuticle surface. Evolutionary origin analysis of BSM suggests that brochosomes likely originated from a process involving duplication–divergence. Our study reveals that leafhoppers employ a unique camouflage strategy by secreting brochosomes as antireflection nanocoatings, enabling them to evade natural predators and contributing to their evolutionary success.https://elifesciences.org/articles/99639brochosomeleafhoppercamouflage
spellingShingle Wei Wu
Qianzhuo Mao
Zhuang-Xin Ye
Zhenfeng Liao
Hong-Wei Shan
Jun-Min Li
Chuan-Xi Zhang
Jian-Ping Chen
Brochosomes as an antireflective camouflage coating for leafhoppers
eLife
brochosome
leafhopper
camouflage
title Brochosomes as an antireflective camouflage coating for leafhoppers
title_full Brochosomes as an antireflective camouflage coating for leafhoppers
title_fullStr Brochosomes as an antireflective camouflage coating for leafhoppers
title_full_unstemmed Brochosomes as an antireflective camouflage coating for leafhoppers
title_short Brochosomes as an antireflective camouflage coating for leafhoppers
title_sort brochosomes as an antireflective camouflage coating for leafhoppers
topic brochosome
leafhopper
camouflage
url https://elifesciences.org/articles/99639
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