Progression of Plasmodium berghei through Anopheles stephensi is density-dependent.
It is well documented that the density of Plasmodium in its vertebrate host modulates the physiological response induced; this in turn regulates parasite survival and transmission. It is less clear that parasite density in the mosquito regulates survival and transmission of this important pathogen....
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2007-12-01
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| Series: | PLoS Pathogens |
| Online Access: | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.0030195&type=printable |
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| author | Robert E Sinden Emma J Dawes Yasmene Alavi Joanna Waldock Olivia Finney Jacqui Mendoza Geoff A Butcher Laura Andrews Adrian V Hill Sarah C Gilbert María-Gloria Basáñez |
| author_facet | Robert E Sinden Emma J Dawes Yasmene Alavi Joanna Waldock Olivia Finney Jacqui Mendoza Geoff A Butcher Laura Andrews Adrian V Hill Sarah C Gilbert María-Gloria Basáñez |
| author_sort | Robert E Sinden |
| collection | DOAJ |
| description | It is well documented that the density of Plasmodium in its vertebrate host modulates the physiological response induced; this in turn regulates parasite survival and transmission. It is less clear that parasite density in the mosquito regulates survival and transmission of this important pathogen. Numerous studies have described conversion rates of Plasmodium from one life stage to the next within the mosquito, yet few have considered that these rates might vary with parasite density. Here we establish infections with defined numbers of the rodent malaria parasite Plasmodium berghei to examine how parasite density at each stage of development (gametocytes; ookinetes; oocysts and sporozoites) influences development to the ensuing stage in Anopheles stephensi, and thus the delivery of infectious sporozoites to the vertebrate host. We show that every developmental transition exhibits strong density dependence, with numbers of the ensuing stages saturating at high density. We further show that when fed ookinetes at very low densities, oocyst development is facilitated by increasing ookinete number (i.e., the efficiency of ookinete-oocyst transformation follows a sigmoid relationship). We discuss how observations on this model system generate important hypotheses for the understanding of malaria biology, and how these might guide the rational analysis of interventions against the transmission of the malaria parasites of humans by their diverse vector species. |
| format | Article |
| id | doaj-art-8953d27e55dd4aaca3e26bbbfe106a7a |
| institution | OA Journals |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2007-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-8953d27e55dd4aaca3e26bbbfe106a7a2025-08-20T02:38:27ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742007-12-01312e19510.1371/journal.ppat.0030195Progression of Plasmodium berghei through Anopheles stephensi is density-dependent.Robert E SindenEmma J DawesYasmene AlaviJoanna WaldockOlivia FinneyJacqui MendozaGeoff A ButcherLaura AndrewsAdrian V HillSarah C GilbertMaría-Gloria BasáñezIt is well documented that the density of Plasmodium in its vertebrate host modulates the physiological response induced; this in turn regulates parasite survival and transmission. It is less clear that parasite density in the mosquito regulates survival and transmission of this important pathogen. Numerous studies have described conversion rates of Plasmodium from one life stage to the next within the mosquito, yet few have considered that these rates might vary with parasite density. Here we establish infections with defined numbers of the rodent malaria parasite Plasmodium berghei to examine how parasite density at each stage of development (gametocytes; ookinetes; oocysts and sporozoites) influences development to the ensuing stage in Anopheles stephensi, and thus the delivery of infectious sporozoites to the vertebrate host. We show that every developmental transition exhibits strong density dependence, with numbers of the ensuing stages saturating at high density. We further show that when fed ookinetes at very low densities, oocyst development is facilitated by increasing ookinete number (i.e., the efficiency of ookinete-oocyst transformation follows a sigmoid relationship). We discuss how observations on this model system generate important hypotheses for the understanding of malaria biology, and how these might guide the rational analysis of interventions against the transmission of the malaria parasites of humans by their diverse vector species.https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.0030195&type=printable |
| spellingShingle | Robert E Sinden Emma J Dawes Yasmene Alavi Joanna Waldock Olivia Finney Jacqui Mendoza Geoff A Butcher Laura Andrews Adrian V Hill Sarah C Gilbert María-Gloria Basáñez Progression of Plasmodium berghei through Anopheles stephensi is density-dependent. PLoS Pathogens |
| title | Progression of Plasmodium berghei through Anopheles stephensi is density-dependent. |
| title_full | Progression of Plasmodium berghei through Anopheles stephensi is density-dependent. |
| title_fullStr | Progression of Plasmodium berghei through Anopheles stephensi is density-dependent. |
| title_full_unstemmed | Progression of Plasmodium berghei through Anopheles stephensi is density-dependent. |
| title_short | Progression of Plasmodium berghei through Anopheles stephensi is density-dependent. |
| title_sort | progression of plasmodium berghei through anopheles stephensi is density dependent |
| url | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.0030195&type=printable |
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