Functionalized Antifouling Polymer Brushes for Biospecific Surfaces
Abstract Biosensors have become integrated into our lives. Current technology requires biosensors not only to have high sensitivity but also to have high specificity for one target, while repelling all other molecules and materials in the biological medium. These goals are met by surfaces that combi...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2025-07-01
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| Series: | Advanced Materials Interfaces |
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| Online Access: | https://doi.org/10.1002/admi.202400955 |
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| author | Erik J. Postma Luc Scheres Sissi deBeer Andriy R. Kuzmyn Han Zuilhof |
| author_facet | Erik J. Postma Luc Scheres Sissi deBeer Andriy R. Kuzmyn Han Zuilhof |
| author_sort | Erik J. Postma |
| collection | DOAJ |
| description | Abstract Biosensors have become integrated into our lives. Current technology requires biosensors not only to have high sensitivity but also to have high specificity for one target, while repelling all other molecules and materials in the biological medium. These goals are met by surfaces that combine a biorecognition element and a high‐quality antifouling layer. In this review, we largely focus on polymer brushes that are grafted from the surface, as these are known to exhibit excellent antifouling properties. We also discuss how to functionalize these with biorecognition elements. Based on the current research on antifouling brushes, we recommend using poly(2‐hydroxypropylmethacrylamide) (HPMAA) and/or poly(carboxybetainemethacrylamide) (CBMAA) brushes, with a thickness between 20–30 nm. Furthermore, we note the importance of high polymer chain densities in such brushes and highlight that a proper comparison requires, among others, similar pre‐treatments. These antifouling brushes are biospecific after receptors are integrated with efficient coupling strategies. Here the opportunities and limitations of frequently used approaches of antifouling polymer brushes within biosensors are highlighted. Also, with the resulting combination of high specificity and low (bio‐)chemical noise levels, we envision an increase in the incorporation of novel polymer brushes for the development of stable biospecific sensors. |
| format | Article |
| id | doaj-art-89510c8285bd4fb6a1fde338ebf005e0 |
| institution | Kabale University |
| issn | 2196-7350 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced Materials Interfaces |
| spelling | doaj-art-89510c8285bd4fb6a1fde338ebf005e02025-08-20T03:31:40ZengWiley-VCHAdvanced Materials Interfaces2196-73502025-07-011214n/an/a10.1002/admi.202400955Functionalized Antifouling Polymer Brushes for Biospecific SurfacesErik J. Postma0Luc Scheres1Sissi deBeer2Andriy R. Kuzmyn3Han Zuilhof4Department of Molecules & Materials MESA+ Institute University of Twente Enschede 7500AE The NetherlandsSurfix BV Agro Business Park 2 Wageningen 6708 PW The NetherlandsDepartment of Molecules & Materials MESA+ Institute University of Twente Enschede 7500AE The NetherlandsDepartment of Molecules & Materials MESA+ Institute University of Twente Enschede 7500AE The NetherlandsLaboratory of Organic Chemistry Wageningen University Stippeneng 4 Wageningen 6708 WE The NetherlandsAbstract Biosensors have become integrated into our lives. Current technology requires biosensors not only to have high sensitivity but also to have high specificity for one target, while repelling all other molecules and materials in the biological medium. These goals are met by surfaces that combine a biorecognition element and a high‐quality antifouling layer. In this review, we largely focus on polymer brushes that are grafted from the surface, as these are known to exhibit excellent antifouling properties. We also discuss how to functionalize these with biorecognition elements. Based on the current research on antifouling brushes, we recommend using poly(2‐hydroxypropylmethacrylamide) (HPMAA) and/or poly(carboxybetainemethacrylamide) (CBMAA) brushes, with a thickness between 20–30 nm. Furthermore, we note the importance of high polymer chain densities in such brushes and highlight that a proper comparison requires, among others, similar pre‐treatments. These antifouling brushes are biospecific after receptors are integrated with efficient coupling strategies. Here the opportunities and limitations of frequently used approaches of antifouling polymer brushes within biosensors are highlighted. Also, with the resulting combination of high specificity and low (bio‐)chemical noise levels, we envision an increase in the incorporation of novel polymer brushes for the development of stable biospecific sensors.https://doi.org/10.1002/admi.202400955antifoulingbiofunctionalizationbiosensorsbiospecific surfacesfoulingpolymer brushes |
| spellingShingle | Erik J. Postma Luc Scheres Sissi deBeer Andriy R. Kuzmyn Han Zuilhof Functionalized Antifouling Polymer Brushes for Biospecific Surfaces Advanced Materials Interfaces antifouling biofunctionalization biosensors biospecific surfaces fouling polymer brushes |
| title | Functionalized Antifouling Polymer Brushes for Biospecific Surfaces |
| title_full | Functionalized Antifouling Polymer Brushes for Biospecific Surfaces |
| title_fullStr | Functionalized Antifouling Polymer Brushes for Biospecific Surfaces |
| title_full_unstemmed | Functionalized Antifouling Polymer Brushes for Biospecific Surfaces |
| title_short | Functionalized Antifouling Polymer Brushes for Biospecific Surfaces |
| title_sort | functionalized antifouling polymer brushes for biospecific surfaces |
| topic | antifouling biofunctionalization biosensors biospecific surfaces fouling polymer brushes |
| url | https://doi.org/10.1002/admi.202400955 |
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