Physicochemical and Antibacterial Properties of Chitosan Extracted from Waste Shrimp Shells
This research aims to study the production of chitosan from shrimp shell (Litopenaeus vannamei) of waste origin using two chemical methodologies involving demineralization, deproteinization, and the degree of deacetylation. The evaluation of the quality of chitosan from waste shrimp shells includes...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2016/5127515 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849400452128440320 |
|---|---|
| author | José Carlos Vilar Junior Daylin Rubio Ribeaux Carlos Alberto Alves da Silva Galba Maria De Campos-Takaki |
| author_facet | José Carlos Vilar Junior Daylin Rubio Ribeaux Carlos Alberto Alves da Silva Galba Maria De Campos-Takaki |
| author_sort | José Carlos Vilar Junior |
| collection | DOAJ |
| description | This research aims to study the production of chitosan from shrimp shell (Litopenaeus vannamei) of waste origin using two chemical methodologies involving demineralization, deproteinization, and the degree of deacetylation. The evaluation of the quality of chitosan from waste shrimp shells includes parameters for the yield, physical chemistry characteristics by infrared spectroscopy (FT-IR), the degree of deacetylation, and antibacterial activity. The results showed (by Method 1) extraction yields for chitin of 33% and for chitosan of 49% and a 76% degree of deacetylation. Chitosan obtained by Method 2 was more efficient: chitin (36%) and chitosan (63%), with a high degree of deacetylation (81.7%). The antibacterial activity was tested against Gram-negative bacteria (Stenotrophomonas maltophilia and Enterobacter cloacae) and Gram-positive Bacillus subtilis and the Minimum Inhibitory Concentrations (MIC) and the Minimum Bactericidal Concentration (MBC) were determined. Method 2 showed that extracted chitosan has good antimicrobial potential against Gram-positive and Gram-negative bacteria and that the process is viable. |
| format | Article |
| id | doaj-art-894dfbe4907741618699bc313efb6932 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-894dfbe4907741618699bc313efb69322025-08-20T03:38:02ZengWileyInternational Journal of Microbiology1687-918X1687-91982016-01-01201610.1155/2016/51275155127515Physicochemical and Antibacterial Properties of Chitosan Extracted from Waste Shrimp ShellsJosé Carlos Vilar Junior0Daylin Rubio Ribeaux1Carlos Alberto Alves da Silva2Galba Maria De Campos-Takaki3Northeast Network for Biotechnology, Federal Rural University of Pernambuco, 52171-900 Recife, PE, BrazilNucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50050-590 Recife, PE, BrazilNucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50050-590 Recife, PE, BrazilNucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, 50050-590 Recife, PE, BrazilThis research aims to study the production of chitosan from shrimp shell (Litopenaeus vannamei) of waste origin using two chemical methodologies involving demineralization, deproteinization, and the degree of deacetylation. The evaluation of the quality of chitosan from waste shrimp shells includes parameters for the yield, physical chemistry characteristics by infrared spectroscopy (FT-IR), the degree of deacetylation, and antibacterial activity. The results showed (by Method 1) extraction yields for chitin of 33% and for chitosan of 49% and a 76% degree of deacetylation. Chitosan obtained by Method 2 was more efficient: chitin (36%) and chitosan (63%), with a high degree of deacetylation (81.7%). The antibacterial activity was tested against Gram-negative bacteria (Stenotrophomonas maltophilia and Enterobacter cloacae) and Gram-positive Bacillus subtilis and the Minimum Inhibitory Concentrations (MIC) and the Minimum Bactericidal Concentration (MBC) were determined. Method 2 showed that extracted chitosan has good antimicrobial potential against Gram-positive and Gram-negative bacteria and that the process is viable.http://dx.doi.org/10.1155/2016/5127515 |
| spellingShingle | José Carlos Vilar Junior Daylin Rubio Ribeaux Carlos Alberto Alves da Silva Galba Maria De Campos-Takaki Physicochemical and Antibacterial Properties of Chitosan Extracted from Waste Shrimp Shells International Journal of Microbiology |
| title | Physicochemical and Antibacterial Properties of Chitosan Extracted from Waste Shrimp Shells |
| title_full | Physicochemical and Antibacterial Properties of Chitosan Extracted from Waste Shrimp Shells |
| title_fullStr | Physicochemical and Antibacterial Properties of Chitosan Extracted from Waste Shrimp Shells |
| title_full_unstemmed | Physicochemical and Antibacterial Properties of Chitosan Extracted from Waste Shrimp Shells |
| title_short | Physicochemical and Antibacterial Properties of Chitosan Extracted from Waste Shrimp Shells |
| title_sort | physicochemical and antibacterial properties of chitosan extracted from waste shrimp shells |
| url | http://dx.doi.org/10.1155/2016/5127515 |
| work_keys_str_mv | AT josecarlosvilarjunior physicochemicalandantibacterialpropertiesofchitosanextractedfromwasteshrimpshells AT daylinrubioribeaux physicochemicalandantibacterialpropertiesofchitosanextractedfromwasteshrimpshells AT carlosalbertoalvesdasilva physicochemicalandantibacterialpropertiesofchitosanextractedfromwasteshrimpshells AT galbamariadecampostakaki physicochemicalandantibacterialpropertiesofchitosanextractedfromwasteshrimpshells |