Amino acid distribution in blood following high-intensity interval exercise: a preliminary study
Abstract This study investigated the effect of high-intensity interval exercise on total and individual amino acid concentrations in red blood cells (RBCs) and plasma. Seven males (31 ± 13 yr) provided venous blood samples at rest, immediately and 15 min and 30 min following an 8-min high-intensity...
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| Format: | Article |
| Language: | English |
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Springer
2024-02-01
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| Series: | Amino Acids |
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| Online Access: | https://doi.org/10.1007/s00726-023-03378-y |
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| author | Nattai Borges Thomas M. Doering Grace Murphy Margaret Macdonald Richard H. Dunstan |
| author_facet | Nattai Borges Thomas M. Doering Grace Murphy Margaret Macdonald Richard H. Dunstan |
| author_sort | Nattai Borges |
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| description | Abstract This study investigated the effect of high-intensity interval exercise on total and individual amino acid concentrations in red blood cells (RBCs) and plasma. Seven males (31 ± 13 yr) provided venous blood samples at rest, immediately and 15 min and 30 min following an 8-min high-intensity exercise bout. The exercise bout was 16 × 15 s cycle efforts at 0.4N/kg of body mass and 90 rpm, interspersed with 15 s passive recovery. Total and individual amino acid concentrations of RBC and plasma and blood cell parameters were analysed. No significant differences for total amino acid concentrations between RBC and plasma were found. Individual amino acid analyses showed significant interaction effects for alanine and α-aminoadipic acid (P < 0.05), with plasma alanine significantly increased from baseline across the recovery period (P < 0.001). Blood fraction (group) effects showed greater concentrations of glycine, serine, asparagine, aspartic acid, glutamic acid, α-aminoadipic acid and ornithine in RBC, while greater concentrations of alanine, α-aminobutyric acid, valine, leucine, isoleucine, threonine, proline, phenylalanine, glutamine, tryptophan and cystine were found in plasma (P < 0.05). Comparable levels of histidine, lysine and tyrosine were observed between blood fractions. Significant differences in the variation of total amino acids in RBC were reported with higher variance at rest compared to following exercise (P = 0.01). Haemoglobin, pack cell volume and white blood cell count significantly increased immediately following exercise (P < 0.05) but returned to baseline after 15 min recovery. These results support the notion of individualised amino acid transportation roles for RBC and plasma during exercise. |
| format | Article |
| id | doaj-art-894cfe5da89c4ac59a00fefe769f794b |
| institution | OA Journals |
| issn | 1438-2199 |
| language | English |
| publishDate | 2024-02-01 |
| publisher | Springer |
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| series | Amino Acids |
| spelling | doaj-art-894cfe5da89c4ac59a00fefe769f794b2025-08-20T01:57:16ZengSpringerAmino Acids1438-21992024-02-015611810.1007/s00726-023-03378-yAmino acid distribution in blood following high-intensity interval exercise: a preliminary studyNattai Borges0Thomas M. Doering1Grace Murphy2Margaret Macdonald3Richard H. Dunstan4Faculty of Medicine and Health, School of Health Sciences, University of New South WalesSchool of Health, Medical and Applied Sciences, Central Queensland UniversitySchool of Environmental and Life Sciences, University of NewcastleSchool of Environmental and Life Sciences, University of NewcastleInnovAAte Pty LtdAbstract This study investigated the effect of high-intensity interval exercise on total and individual amino acid concentrations in red blood cells (RBCs) and plasma. Seven males (31 ± 13 yr) provided venous blood samples at rest, immediately and 15 min and 30 min following an 8-min high-intensity exercise bout. The exercise bout was 16 × 15 s cycle efforts at 0.4N/kg of body mass and 90 rpm, interspersed with 15 s passive recovery. Total and individual amino acid concentrations of RBC and plasma and blood cell parameters were analysed. No significant differences for total amino acid concentrations between RBC and plasma were found. Individual amino acid analyses showed significant interaction effects for alanine and α-aminoadipic acid (P < 0.05), with plasma alanine significantly increased from baseline across the recovery period (P < 0.001). Blood fraction (group) effects showed greater concentrations of glycine, serine, asparagine, aspartic acid, glutamic acid, α-aminoadipic acid and ornithine in RBC, while greater concentrations of alanine, α-aminobutyric acid, valine, leucine, isoleucine, threonine, proline, phenylalanine, glutamine, tryptophan and cystine were found in plasma (P < 0.05). Comparable levels of histidine, lysine and tyrosine were observed between blood fractions. Significant differences in the variation of total amino acids in RBC were reported with higher variance at rest compared to following exercise (P = 0.01). Haemoglobin, pack cell volume and white blood cell count significantly increased immediately following exercise (P < 0.05) but returned to baseline after 15 min recovery. These results support the notion of individualised amino acid transportation roles for RBC and plasma during exercise.https://doi.org/10.1007/s00726-023-03378-yRed Blood CellErythrocyteCyclePlasmaProtein |
| spellingShingle | Nattai Borges Thomas M. Doering Grace Murphy Margaret Macdonald Richard H. Dunstan Amino acid distribution in blood following high-intensity interval exercise: a preliminary study Amino Acids Red Blood Cell Erythrocyte Cycle Plasma Protein |
| title | Amino acid distribution in blood following high-intensity interval exercise: a preliminary study |
| title_full | Amino acid distribution in blood following high-intensity interval exercise: a preliminary study |
| title_fullStr | Amino acid distribution in blood following high-intensity interval exercise: a preliminary study |
| title_full_unstemmed | Amino acid distribution in blood following high-intensity interval exercise: a preliminary study |
| title_short | Amino acid distribution in blood following high-intensity interval exercise: a preliminary study |
| title_sort | amino acid distribution in blood following high intensity interval exercise a preliminary study |
| topic | Red Blood Cell Erythrocyte Cycle Plasma Protein |
| url | https://doi.org/10.1007/s00726-023-03378-y |
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