Temporal changes in ruminal microbiota composition and diversity in dairy cows supplemented with a lactobacilli-based DFM
IntroductionThis study aimed to evaluate the impact of lactobacilli-based direct-fed microbial (DFM) supplementation on the composition and diversity of the ruminal microbiota in dairy cows. Understanding how DFM influences microbial populations can inform strategies to enhance animal health and pro...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-05-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1584959/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850272111491284992 |
|---|---|
| author | Bronwyn E. Campbell Mohammad Mahmudul Hassan Robert J. Moore Timothy Olchowy Martin Soust Rafat Al Jassim John I. Alawneh John I. Alawneh |
| author_facet | Bronwyn E. Campbell Mohammad Mahmudul Hassan Robert J. Moore Timothy Olchowy Martin Soust Rafat Al Jassim John I. Alawneh John I. Alawneh |
| author_sort | Bronwyn E. Campbell |
| collection | DOAJ |
| description | IntroductionThis study aimed to evaluate the impact of lactobacilli-based direct-fed microbial (DFM) supplementation on the composition and diversity of the ruminal microbiota in dairy cows. Understanding how DFM influences microbial populations can inform strategies to enhance animal health and productivity.MethodsOver a 16-month period (September 2021 to January 2023), ruminal fluid samples were collected from fifty dairy cows assigned to either a DFM-supplemented group (DFM; n = 25) or an unsupplemented control group (CON; n = 25). Microbial DNA was extracted and subjected to 16S rRNA gene amplification and sequencing. Microbial diversity was assessed using alpha- and beta-diversity metrics (p < 0.05), and linear discriminant analysis effect size (LEfSe) was employed to identify differentially abundant taxa. Multivariable analyses were used to explore associations with age, average milk production, days in milk (DIM), time, and supplementation.ResultsThe dominant bacterial phyla identified were Bacillota and Bacteroidota, while Methanobacteriaceae was the predominant archaeal family. The DFM group showed significantly higher abundance of genera such as Eubacterium_Q, Atopobium sp. UBA7741, and Sharpea (p < 0.05). Conversely, Bacillus_P_294101 and SFMI01 were more abundant in the CON group. Temporal changes in microbial composition were observed, with significant differences in community diversity and structure between groups over time.DiscussionThese findings demonstrate that lactobacilli-based DFM supplementation can significantly alter the ruminal microbial ecosystem in dairy cows. The observed microbial shifts, including increases in beneficial bacterial taxa, highlight the potential of DFM as a nutritional strategy to modulate rumen function and improve dairy cow performance. |
| format | Article |
| id | doaj-art-894b4be57b04403d97d74d7710ecf008 |
| institution | OA Journals |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-894b4be57b04403d97d74d7710ecf0082025-08-20T01:51:58ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-05-011210.3389/fvets.2025.15849591584959Temporal changes in ruminal microbiota composition and diversity in dairy cows supplemented with a lactobacilli-based DFMBronwyn E. Campbell0Mohammad Mahmudul Hassan1Robert J. Moore2Timothy Olchowy3Martin Soust4Rafat Al Jassim5John I. Alawneh6John I. Alawneh7School of Science, RMIT University, Bundoora, VIC, AustraliaSchool of Veterinary Science, The University of Queensland, Gatton, QLD, AustraliaSchool of Science, RMIT University, Bundoora, VIC, AustraliaFaculty of Veterinary Medicine, University of Calgary, Calgary, AB, CanadaTerragen Biotech Pty Ltd., Coolum Beach, QLD, AustraliaQueensland Alliance for Agriculture and Food Innovation, Saint Lucia, QLD, AustraliaSchool of Veterinary Science, The University of Queensland, Gatton, QLD, AustraliaDepartment of Primary Industries, Plant Biosecurity and Product Integrity, Biosecurity Queensland, Brisbane, QLD, AustraliaIntroductionThis study aimed to evaluate the impact of lactobacilli-based direct-fed microbial (DFM) supplementation on the composition and diversity of the ruminal microbiota in dairy cows. Understanding how DFM influences microbial populations can inform strategies to enhance animal health and productivity.MethodsOver a 16-month period (September 2021 to January 2023), ruminal fluid samples were collected from fifty dairy cows assigned to either a DFM-supplemented group (DFM; n = 25) or an unsupplemented control group (CON; n = 25). Microbial DNA was extracted and subjected to 16S rRNA gene amplification and sequencing. Microbial diversity was assessed using alpha- and beta-diversity metrics (p < 0.05), and linear discriminant analysis effect size (LEfSe) was employed to identify differentially abundant taxa. Multivariable analyses were used to explore associations with age, average milk production, days in milk (DIM), time, and supplementation.ResultsThe dominant bacterial phyla identified were Bacillota and Bacteroidota, while Methanobacteriaceae was the predominant archaeal family. The DFM group showed significantly higher abundance of genera such as Eubacterium_Q, Atopobium sp. UBA7741, and Sharpea (p < 0.05). Conversely, Bacillus_P_294101 and SFMI01 were more abundant in the CON group. Temporal changes in microbial composition were observed, with significant differences in community diversity and structure between groups over time.DiscussionThese findings demonstrate that lactobacilli-based DFM supplementation can significantly alter the ruminal microbial ecosystem in dairy cows. The observed microbial shifts, including increases in beneficial bacterial taxa, highlight the potential of DFM as a nutritional strategy to modulate rumen function and improve dairy cow performance.https://www.frontiersin.org/articles/10.3389/fvets.2025.1584959/fullruminal microbiotadairy cowsdirect-fed microbialmicrobial diversitytemporal changes |
| spellingShingle | Bronwyn E. Campbell Mohammad Mahmudul Hassan Robert J. Moore Timothy Olchowy Martin Soust Rafat Al Jassim John I. Alawneh John I. Alawneh Temporal changes in ruminal microbiota composition and diversity in dairy cows supplemented with a lactobacilli-based DFM Frontiers in Veterinary Science ruminal microbiota dairy cows direct-fed microbial microbial diversity temporal changes |
| title | Temporal changes in ruminal microbiota composition and diversity in dairy cows supplemented with a lactobacilli-based DFM |
| title_full | Temporal changes in ruminal microbiota composition and diversity in dairy cows supplemented with a lactobacilli-based DFM |
| title_fullStr | Temporal changes in ruminal microbiota composition and diversity in dairy cows supplemented with a lactobacilli-based DFM |
| title_full_unstemmed | Temporal changes in ruminal microbiota composition and diversity in dairy cows supplemented with a lactobacilli-based DFM |
| title_short | Temporal changes in ruminal microbiota composition and diversity in dairy cows supplemented with a lactobacilli-based DFM |
| title_sort | temporal changes in ruminal microbiota composition and diversity in dairy cows supplemented with a lactobacilli based dfm |
| topic | ruminal microbiota dairy cows direct-fed microbial microbial diversity temporal changes |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1584959/full |
| work_keys_str_mv | AT bronwynecampbell temporalchangesinruminalmicrobiotacompositionanddiversityindairycowssupplementedwithalactobacillibaseddfm AT mohammadmahmudulhassan temporalchangesinruminalmicrobiotacompositionanddiversityindairycowssupplementedwithalactobacillibaseddfm AT robertjmoore temporalchangesinruminalmicrobiotacompositionanddiversityindairycowssupplementedwithalactobacillibaseddfm AT timothyolchowy temporalchangesinruminalmicrobiotacompositionanddiversityindairycowssupplementedwithalactobacillibaseddfm AT martinsoust temporalchangesinruminalmicrobiotacompositionanddiversityindairycowssupplementedwithalactobacillibaseddfm AT rafataljassim temporalchangesinruminalmicrobiotacompositionanddiversityindairycowssupplementedwithalactobacillibaseddfm AT johnialawneh temporalchangesinruminalmicrobiotacompositionanddiversityindairycowssupplementedwithalactobacillibaseddfm AT johnialawneh temporalchangesinruminalmicrobiotacompositionanddiversityindairycowssupplementedwithalactobacillibaseddfm |