Simultaneous HPLC Determination of Methocarbamol, Paracetamol and Diclofenac Sodium

A rapid and simple high performance liquid chromatography method was developed and validated at λ=275 nm for simultaneous determination of methocarbamol, paracetamol and diclofenac sodium (lamotrigine as an internal standard) from pharmaceutical preparation. The separation was performed using methan...

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Bibliographic Details
Main Authors: Deshmukh Hafsa, S. Chanda, Pradnya J. Prabhu
Format: Article
Language:English
Published: Wiley 2011-01-01
Series:E-Journal of Chemistry
Online Access:http://dx.doi.org/10.1155/2011/614748
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Summary:A rapid and simple high performance liquid chromatography method was developed and validated at λ=275 nm for simultaneous determination of methocarbamol, paracetamol and diclofenac sodium (lamotrigine as an internal standard) from pharmaceutical preparation. The separation was performed using methanol: water: GAA, in the ratio of 400:600:05 (v/v) as the mobile phase. The detector response was linear in the range of 5 to 45 μg, 3.25 to 29.25 μg, 0.5 to 4.5 μg for methocarbamol, paracetamol and diclofenac sodium respectively. The percentage assay of methocarbamol, paracetamol and diclofenac sodium was found between 100, 99.76 and 99.31% respectively. The described method has the advantage of being rapid and easy hence it can be applied for routine quality control analysis.
ISSN:0973-4945
2090-9810