Formyl-peptide receptor type 2 activation mitigates heart and lung damage in inflammatory arthritis

Abstract Rheumatoid arthritis (RA) is associated with heart and lung dysfunction. Current therapies fail to attenuate such complications. Here, we identify formyl-peptide receptor type 2 (FPR2) as a therapeutic target to treat heart and lung dysfunction associated with inflammatory arthritis. Arthri...

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Main Authors: Andreas Margraf, Jianmin Chen, Marilena Christoforou, Pol Claria-Ribas, Ayda Henriques Schneider, Chiara Cecconello, Weifeng Bu, Paul R C Imbert, Thomas D Wright, Stefan Russo, Isobel A Blacksell, Duco S Koenis, Jesmond Dalli, John A Lupisella, Nicholas R Wurtz, Ricardo A Garcia, Dianne Cooper, Lucy V Norling, Mauro Perretti
Format: Article
Language:English
Published: Springer Nature 2025-04-01
Series:EMBO Molecular Medicine
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Online Access:https://doi.org/10.1038/s44321-025-00227-1
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author Andreas Margraf
Jianmin Chen
Marilena Christoforou
Pol Claria-Ribas
Ayda Henriques Schneider
Chiara Cecconello
Weifeng Bu
Paul R C Imbert
Thomas D Wright
Stefan Russo
Isobel A Blacksell
Duco S Koenis
Jesmond Dalli
John A Lupisella
Nicholas R Wurtz
Ricardo A Garcia
Dianne Cooper
Lucy V Norling
Mauro Perretti
author_facet Andreas Margraf
Jianmin Chen
Marilena Christoforou
Pol Claria-Ribas
Ayda Henriques Schneider
Chiara Cecconello
Weifeng Bu
Paul R C Imbert
Thomas D Wright
Stefan Russo
Isobel A Blacksell
Duco S Koenis
Jesmond Dalli
John A Lupisella
Nicholas R Wurtz
Ricardo A Garcia
Dianne Cooper
Lucy V Norling
Mauro Perretti
author_sort Andreas Margraf
collection DOAJ
description Abstract Rheumatoid arthritis (RA) is associated with heart and lung dysfunction. Current therapies fail to attenuate such complications. Here, we identify formyl-peptide receptor type 2 (FPR2) as a therapeutic target to treat heart and lung dysfunction associated with inflammatory arthritis. Arthritic mice on high levels of dietary homocysteine develop cardiac diastolic dysfunction and reduced lung compliance, mirroring two comorbidities in RA. Therapeutic administration of a small molecule FPR2 agonist (BMS986235) to hyper-homocysteine arthritic mice prevented diastolic dysfunction (monitored by echocardiography) and restored lung compliance. These tissue-specific effects were secondary to reduced neutrophil infiltration, modulation of fibroblast activation and phenotype (in the heart) and attenuation of monocyte and macrophage numbers (in the lung). A dual FPR1/2 agonist (compound 43) failed to prevent the reduction in lung compliance of arthritic mice and promoted the accumulation of inflammatory monocytes and pro-fibrotic macrophages in lung parenchyma. This cellular response lies downstream of FPR1-mediated potentiation of CCL2-dependent monocyte chemotaxis and activation. This finding supports the therapeutic development of selective FPR2 agonists to mitigate two impactful comorbidities associated with inflammatory arthritides.
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spelling doaj-art-8922f9ca68ee48b7bff042e9da2a6c532025-08-20T01:51:30ZengSpringer NatureEMBO Molecular Medicine1757-46842025-04-011751153118310.1038/s44321-025-00227-1Formyl-peptide receptor type 2 activation mitigates heart and lung damage in inflammatory arthritisAndreas Margraf0Jianmin Chen1Marilena Christoforou2Pol Claria-Ribas3Ayda Henriques Schneider4Chiara Cecconello5Weifeng Bu6Paul R C Imbert7Thomas D Wright8Stefan Russo9Isobel A Blacksell10Duco S Koenis11Jesmond Dalli12John A Lupisella13Nicholas R Wurtz14Ricardo A Garcia15Dianne Cooper16Lucy V Norling17Mauro Perretti18William Harvey Research Institute, Faculty of Medicine and Dentistry, Queen Mary University of LondonWilliam Harvey Research Institute, Faculty of Medicine and Dentistry, Queen Mary University of LondonWilliam Harvey Research Institute, Faculty of Medicine and Dentistry, Queen Mary University of LondonWilliam Harvey Research Institute, Faculty of Medicine and Dentistry, Queen Mary University of LondonWilliam Harvey Research Institute, Faculty of Medicine and Dentistry, Queen Mary University of LondonWilliam Harvey Research Institute, Faculty of Medicine and Dentistry, Queen Mary University of LondonWilliam Harvey Research Institute, Faculty of Medicine and Dentistry, Queen Mary University of LondonWilliam Harvey Research Institute, Faculty of Medicine and Dentistry, Queen Mary University of LondonWilliam Harvey Research Institute, Faculty of Medicine and Dentistry, Queen Mary University of LondonWilliam Harvey Research Institute, Faculty of Medicine and Dentistry, Queen Mary University of LondonWilliam Harvey Research Institute, Faculty of Medicine and Dentistry, Queen Mary University of LondonWilliam Harvey Research Institute, Faculty of Medicine and Dentistry, Queen Mary University of LondonWilliam Harvey Research Institute, Faculty of Medicine and Dentistry, Queen Mary University of LondonDepartment of Cardiovascular and Fibrosis Drug Discovery, Bristol Myers SquibbDepartment of Cardiovascular and Fibrosis Drug Discovery, Bristol Myers SquibbDepartment of Cardiovascular and Fibrosis Drug Discovery, Bristol Myers SquibbWilliam Harvey Research Institute, Faculty of Medicine and Dentistry, Queen Mary University of LondonWilliam Harvey Research Institute, Faculty of Medicine and Dentistry, Queen Mary University of LondonWilliam Harvey Research Institute, Faculty of Medicine and Dentistry, Queen Mary University of LondonAbstract Rheumatoid arthritis (RA) is associated with heart and lung dysfunction. Current therapies fail to attenuate such complications. Here, we identify formyl-peptide receptor type 2 (FPR2) as a therapeutic target to treat heart and lung dysfunction associated with inflammatory arthritis. Arthritic mice on high levels of dietary homocysteine develop cardiac diastolic dysfunction and reduced lung compliance, mirroring two comorbidities in RA. Therapeutic administration of a small molecule FPR2 agonist (BMS986235) to hyper-homocysteine arthritic mice prevented diastolic dysfunction (monitored by echocardiography) and restored lung compliance. These tissue-specific effects were secondary to reduced neutrophil infiltration, modulation of fibroblast activation and phenotype (in the heart) and attenuation of monocyte and macrophage numbers (in the lung). A dual FPR1/2 agonist (compound 43) failed to prevent the reduction in lung compliance of arthritic mice and promoted the accumulation of inflammatory monocytes and pro-fibrotic macrophages in lung parenchyma. This cellular response lies downstream of FPR1-mediated potentiation of CCL2-dependent monocyte chemotaxis and activation. This finding supports the therapeutic development of selective FPR2 agonists to mitigate two impactful comorbidities associated with inflammatory arthritides.https://doi.org/10.1038/s44321-025-00227-1Resolution PharmacologyPro-resolving GPCRHFpEFLung InjuryRheumatoid Arthritis
spellingShingle Andreas Margraf
Jianmin Chen
Marilena Christoforou
Pol Claria-Ribas
Ayda Henriques Schneider
Chiara Cecconello
Weifeng Bu
Paul R C Imbert
Thomas D Wright
Stefan Russo
Isobel A Blacksell
Duco S Koenis
Jesmond Dalli
John A Lupisella
Nicholas R Wurtz
Ricardo A Garcia
Dianne Cooper
Lucy V Norling
Mauro Perretti
Formyl-peptide receptor type 2 activation mitigates heart and lung damage in inflammatory arthritis
EMBO Molecular Medicine
Resolution Pharmacology
Pro-resolving GPCR
HFpEF
Lung Injury
Rheumatoid Arthritis
title Formyl-peptide receptor type 2 activation mitigates heart and lung damage in inflammatory arthritis
title_full Formyl-peptide receptor type 2 activation mitigates heart and lung damage in inflammatory arthritis
title_fullStr Formyl-peptide receptor type 2 activation mitigates heart and lung damage in inflammatory arthritis
title_full_unstemmed Formyl-peptide receptor type 2 activation mitigates heart and lung damage in inflammatory arthritis
title_short Formyl-peptide receptor type 2 activation mitigates heart and lung damage in inflammatory arthritis
title_sort formyl peptide receptor type 2 activation mitigates heart and lung damage in inflammatory arthritis
topic Resolution Pharmacology
Pro-resolving GPCR
HFpEF
Lung Injury
Rheumatoid Arthritis
url https://doi.org/10.1038/s44321-025-00227-1
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