Cytotoxicity of the Aqueous Extract and Organic Fractions from Origanum majorana on Human Breast Cell Line MDA-MB-231 and Human Colon Cell Line HT-29
The toxicity of the aqueous extract of Origanum majorana was tested (5 and 10 g/kg) in albino mice. No symptoms of toxicity or mortality were observed. The mice survived being active and healthy during all 14 days of observation. In addition, the weight measurement of the left and right kidneys, hea...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Advances in Pharmacological Sciences |
| Online Access: | http://dx.doi.org/10.1155/2018/3297193 |
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| author | Hanane Makrane Mouhcine El Messaoudi Ahmed Melhaoui Mohammed El Mzibri Laila Benbacer Mohammed Aziz |
| author_facet | Hanane Makrane Mouhcine El Messaoudi Ahmed Melhaoui Mohammed El Mzibri Laila Benbacer Mohammed Aziz |
| author_sort | Hanane Makrane |
| collection | DOAJ |
| description | The toxicity of the aqueous extract of Origanum majorana was tested (5 and 10 g/kg) in albino mice. No symptoms of toxicity or mortality were observed. The mice survived being active and healthy during all 14 days of observation. In addition, the weight measurement of the left and right kidneys, heart, and liver shows no significant difference between the control, 5 g/kg, and 10 g/kg. All extracts (aqueous, petroleum ether, dichloromethane, ethyl acetate, methanolic, and depleted aqueous extracts) of Origanum majorana tested against both types of cancer cells showed a more pronounced cytotoxic effect against breast cell line MDA-MB-231 than colon cells line HT-29 cells. The most marked effect is that of the ethyl acetate extract with IC50 30.90 ± 1.39 and 50.11 ± 1.44 (µg/ml), respectively. HPLC analysis of extracts from Origanum majorana showed that this plant contained polyphenols and flavonoids, which may be responsible for the biological activities found. |
| format | Article |
| id | doaj-art-891cd96ea0a84511b1422a0ebd1bcf3b |
| institution | OA Journals |
| issn | 1687-6334 1687-6342 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Pharmacological Sciences |
| spelling | doaj-art-891cd96ea0a84511b1422a0ebd1bcf3b2025-08-20T02:09:10ZengWileyAdvances in Pharmacological Sciences1687-63341687-63422018-01-01201810.1155/2018/32971933297193Cytotoxicity of the Aqueous Extract and Organic Fractions from Origanum majorana on Human Breast Cell Line MDA-MB-231 and Human Colon Cell Line HT-29Hanane Makrane0Mouhcine El Messaoudi1Ahmed Melhaoui2Mohammed El Mzibri3Laila Benbacer4Mohammed Aziz5Laboratory of Physiology, Genetic and Ethnopharmacology, Faculty of Sciences, Mohammed the First University, PB 717, 60000 Oujda, MoroccoBiology Unit and Medical Research CNESTEN, 10001 Rabat, MoroccoLaboratory of Organic Chemistry, Macromolecular and Natural Products, Faculty of Sciences, Mohammed the First University, PB 717, 60000 Oujda, MoroccoBiology Unit and Medical Research CNESTEN, 10001 Rabat, MoroccoBiology Unit and Medical Research CNESTEN, 10001 Rabat, MoroccoLaboratory of Physiology, Genetic and Ethnopharmacology, Faculty of Sciences, Mohammed the First University, PB 717, 60000 Oujda, MoroccoThe toxicity of the aqueous extract of Origanum majorana was tested (5 and 10 g/kg) in albino mice. No symptoms of toxicity or mortality were observed. The mice survived being active and healthy during all 14 days of observation. In addition, the weight measurement of the left and right kidneys, heart, and liver shows no significant difference between the control, 5 g/kg, and 10 g/kg. All extracts (aqueous, petroleum ether, dichloromethane, ethyl acetate, methanolic, and depleted aqueous extracts) of Origanum majorana tested against both types of cancer cells showed a more pronounced cytotoxic effect against breast cell line MDA-MB-231 than colon cells line HT-29 cells. The most marked effect is that of the ethyl acetate extract with IC50 30.90 ± 1.39 and 50.11 ± 1.44 (µg/ml), respectively. HPLC analysis of extracts from Origanum majorana showed that this plant contained polyphenols and flavonoids, which may be responsible for the biological activities found.http://dx.doi.org/10.1155/2018/3297193 |
| spellingShingle | Hanane Makrane Mouhcine El Messaoudi Ahmed Melhaoui Mohammed El Mzibri Laila Benbacer Mohammed Aziz Cytotoxicity of the Aqueous Extract and Organic Fractions from Origanum majorana on Human Breast Cell Line MDA-MB-231 and Human Colon Cell Line HT-29 Advances in Pharmacological Sciences |
| title | Cytotoxicity of the Aqueous Extract and Organic Fractions from Origanum majorana on Human Breast Cell Line MDA-MB-231 and Human Colon Cell Line HT-29 |
| title_full | Cytotoxicity of the Aqueous Extract and Organic Fractions from Origanum majorana on Human Breast Cell Line MDA-MB-231 and Human Colon Cell Line HT-29 |
| title_fullStr | Cytotoxicity of the Aqueous Extract and Organic Fractions from Origanum majorana on Human Breast Cell Line MDA-MB-231 and Human Colon Cell Line HT-29 |
| title_full_unstemmed | Cytotoxicity of the Aqueous Extract and Organic Fractions from Origanum majorana on Human Breast Cell Line MDA-MB-231 and Human Colon Cell Line HT-29 |
| title_short | Cytotoxicity of the Aqueous Extract and Organic Fractions from Origanum majorana on Human Breast Cell Line MDA-MB-231 and Human Colon Cell Line HT-29 |
| title_sort | cytotoxicity of the aqueous extract and organic fractions from origanum majorana on human breast cell line mda mb 231 and human colon cell line ht 29 |
| url | http://dx.doi.org/10.1155/2018/3297193 |
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