Correlation between class 1 integron of Escherichia coli and multidrug resistance in lower respiratory tract infection
Introduction: Class 1 integrons are mobile genetic elements considered to be responsible for the transfer of multidrug resistance. This study aimed to investigate the distribution of type 1 integrons in multidrug-resistantEscherichiacolifrom hospital-acquired lower respiratory tract infections....
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Journal of Infection in Developing Countries
2017-09-01
|
| Series: | Journal of Infection in Developing Countries |
| Subjects: | |
| Online Access: | https://jidc.org/index.php/journal/article/view/8247 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850193362007621632 |
|---|---|
| author | Yu Wang Bingbing Kong Wenping Yang Xin Zhao |
| author_facet | Yu Wang Bingbing Kong Wenping Yang Xin Zhao |
| author_sort | Yu Wang |
| collection | DOAJ |
| description |
Introduction: Class 1 integrons are mobile genetic elements considered to be responsible for the transfer of multidrug resistance. This study aimed to investigate the distribution of type 1 integrons in multidrug-resistantEscherichiacolifrom hospital-acquired lower respiratory tract infections.
Methodology: Clinical strains of E. coli were isolated from patients with hospital-acquired lower respiratory tract infections in the emergency intensive care unit from January to December 2014. Drug sensitivity testing was performed using the Kirby-Bauer method. The combination disk method was used to detect extended-spectrum β-lactamase (ESBL), and polymerase chain reaction (PCR) amplification was used to detect the intI1 gene.
Results: Among 58 E. coli strains, resistance to β-lactam antibiotics ranked as follows: imipenem (0.0%), cefoperazone/sulbactam sodium (25.9%), ceftazidime (37.9%), and cefepime (39.7%); other β-lactam antibiotic resistance rates were all > 50%. The resistance rates to amikacin, ciprofloxacin, gentamicin, and cotrimoxazole were32.8%, 63.8%, 70.7%, and 81.0%, respectively. In total, 31 (53.4%) isolates were positive for class 1 integron and carried 4 different sizes of amplification fragments: 800, 1,600, 1,900, and 2,600 bp. Among 43 ESBL-positive isolates, 27 (62.8%) also carried class 1 integron; among 15 ESBL-negative isolates, 4 carried class 1 integron (26.7%). The positive rate for class 1 integron in ESBL-producing strains was significantly higher than that in non-ESBL-producing strains. The rates of resistance of integron-positive isolates to ceftriaxone, cefotaxime, amikacin, ciprofloxacin, and cotrimoxazole were significantly higher than those in integron-negative isolates.
Conclusions: Class 1 integrons are widely distributed in E. coli and are associated with multidrug resistance.
|
| format | Article |
| id | doaj-art-890b221072274b799b7d5c7fe89c63ff |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2017-09-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-890b221072274b799b7d5c7fe89c63ff2025-08-20T02:14:17ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802017-09-01110810.3855/jidc.8247Correlation between class 1 integron of Escherichia coli and multidrug resistance in lower respiratory tract infectionYu Wang0Bingbing Kong1Wenping Yang2Xin Zhao3Shengjing Hospital of China Medical University, Liaoning province, Shenyang city, ChinaShengjing Hospital of China Medical University, Liaoning province, Shenyang city,ChinaShengjing Hospital of China Medical University, Liaoning province, Shenyang city, ChinaShengjing Hospital of China Medical University, Liaoning province, Shenyang city, China Introduction: Class 1 integrons are mobile genetic elements considered to be responsible for the transfer of multidrug resistance. This study aimed to investigate the distribution of type 1 integrons in multidrug-resistantEscherichiacolifrom hospital-acquired lower respiratory tract infections. Methodology: Clinical strains of E. coli were isolated from patients with hospital-acquired lower respiratory tract infections in the emergency intensive care unit from January to December 2014. Drug sensitivity testing was performed using the Kirby-Bauer method. The combination disk method was used to detect extended-spectrum β-lactamase (ESBL), and polymerase chain reaction (PCR) amplification was used to detect the intI1 gene. Results: Among 58 E. coli strains, resistance to β-lactam antibiotics ranked as follows: imipenem (0.0%), cefoperazone/sulbactam sodium (25.9%), ceftazidime (37.9%), and cefepime (39.7%); other β-lactam antibiotic resistance rates were all > 50%. The resistance rates to amikacin, ciprofloxacin, gentamicin, and cotrimoxazole were32.8%, 63.8%, 70.7%, and 81.0%, respectively. In total, 31 (53.4%) isolates were positive for class 1 integron and carried 4 different sizes of amplification fragments: 800, 1,600, 1,900, and 2,600 bp. Among 43 ESBL-positive isolates, 27 (62.8%) also carried class 1 integron; among 15 ESBL-negative isolates, 4 carried class 1 integron (26.7%). The positive rate for class 1 integron in ESBL-producing strains was significantly higher than that in non-ESBL-producing strains. The rates of resistance of integron-positive isolates to ceftriaxone, cefotaxime, amikacin, ciprofloxacin, and cotrimoxazole were significantly higher than those in integron-negative isolates. Conclusions: Class 1 integrons are widely distributed in E. coli and are associated with multidrug resistance. https://jidc.org/index.php/journal/article/view/8247hospital-acquired infectionHospital-acquired infectionlower respiratory tract infectionLower respiratory tract infectionEscherichia coliIntegron |
| spellingShingle | Yu Wang Bingbing Kong Wenping Yang Xin Zhao Correlation between class 1 integron of Escherichia coli and multidrug resistance in lower respiratory tract infection Journal of Infection in Developing Countries hospital-acquired infection Hospital-acquired infection lower respiratory tract infection Lower respiratory tract infection Escherichia coli Integron |
| title | Correlation between class 1 integron of Escherichia coli and multidrug resistance in lower respiratory tract infection |
| title_full | Correlation between class 1 integron of Escherichia coli and multidrug resistance in lower respiratory tract infection |
| title_fullStr | Correlation between class 1 integron of Escherichia coli and multidrug resistance in lower respiratory tract infection |
| title_full_unstemmed | Correlation between class 1 integron of Escherichia coli and multidrug resistance in lower respiratory tract infection |
| title_short | Correlation between class 1 integron of Escherichia coli and multidrug resistance in lower respiratory tract infection |
| title_sort | correlation between class 1 integron of escherichia coli and multidrug resistance in lower respiratory tract infection |
| topic | hospital-acquired infection Hospital-acquired infection lower respiratory tract infection Lower respiratory tract infection Escherichia coli Integron |
| url | https://jidc.org/index.php/journal/article/view/8247 |
| work_keys_str_mv | AT yuwang correlationbetweenclass1integronofescherichiacoliandmultidrugresistanceinlowerrespiratorytractinfection AT bingbingkong correlationbetweenclass1integronofescherichiacoliandmultidrugresistanceinlowerrespiratorytractinfection AT wenpingyang correlationbetweenclass1integronofescherichiacoliandmultidrugresistanceinlowerrespiratorytractinfection AT xinzhao correlationbetweenclass1integronofescherichiacoliandmultidrugresistanceinlowerrespiratorytractinfection |