DETERMINATION OF DARBEPOETIN ALFA IN RABBIT PLASMA BY ELISA
Method of quantitative determination of darbepoetin alfa in rabbit blood plasma was developed. Quantitative determination was performed by ELISA using commercially available ELISA kits for assay «Erythropoietin ELISA-Best». The method was validated on the following parameters: specificity, linearity...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | Russian |
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LLC Center of Pharmaceutical Analytics (LLC «CPHA»)
2019-01-01
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| Series: | Разработка и регистрация лекарственных средств |
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| Online Access: | https://www.pharmjournal.ru/jour/article/view/176 |
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| author | M. V. Karlina N. M. Faustova O. N. Pozharitskaya V. M. Kosman V. G. Makarov A. N. Shikov |
| author_facet | M. V. Karlina N. M. Faustova O. N. Pozharitskaya V. M. Kosman V. G. Makarov A. N. Shikov |
| author_sort | M. V. Karlina |
| collection | DOAJ |
| description | Method of quantitative determination of darbepoetin alfa in rabbit blood plasma was developed. Quantitative determination was performed by ELISA using commercially available ELISA kits for assay «Erythropoietin ELISA-Best». The method was validated on the following parameters: specificity, linearity, precision, accuracy, and the stability samples. Linear range of methods for determining darbepoetin alfa in the blood plasma of rabbits was 67-2000 pg/mL, the limit of detection - 31 pg/ml. The method can be used for bioequivalence and toxicokinetics studies of drugs of darbepoetin alfa. |
| format | Article |
| id | doaj-art-8906641558e44be6aafcf57756e55aba |
| institution | Kabale University |
| issn | 2305-2066 2658-5049 |
| language | Russian |
| publishDate | 2019-01-01 |
| publisher | LLC Center of Pharmaceutical Analytics (LLC «CPHA») |
| record_format | Article |
| series | Разработка и регистрация лекарственных средств |
| spelling | doaj-art-8906641558e44be6aafcf57756e55aba2025-08-20T03:59:20ZrusLLC Center of Pharmaceutical Analytics (LLC «CPHA»)Разработка и регистрация лекарственных средств2305-20662658-50492019-01-0104204210176DETERMINATION OF DARBEPOETIN ALFA IN RABBIT PLASMA BY ELISAM. V. Karlina0N. M. Faustova1O. N. Pozharitskaya2V. M. Kosman3V. G. Makarov4A. N. Shikov5Saint-Petersburg Institute of PharmacySaint-Petersburg Institute of PharmacySaint-Petersburg Institute of PharmacySaint-Petersburg Institute of PharmacySaint-Petersburg Institute of PharmacySaint-Petersburg Institute of PharmacyMethod of quantitative determination of darbepoetin alfa in rabbit blood plasma was developed. Quantitative determination was performed by ELISA using commercially available ELISA kits for assay «Erythropoietin ELISA-Best». The method was validated on the following parameters: specificity, linearity, precision, accuracy, and the stability samples. Linear range of methods for determining darbepoetin alfa in the blood plasma of rabbits was 67-2000 pg/mL, the limit of detection - 31 pg/ml. The method can be used for bioequivalence and toxicokinetics studies of drugs of darbepoetin alfa.https://www.pharmjournal.ru/jour/article/view/176darbepoetinelisavalidationpharmacokineticsrabbitsplasma |
| spellingShingle | M. V. Karlina N. M. Faustova O. N. Pozharitskaya V. M. Kosman V. G. Makarov A. N. Shikov DETERMINATION OF DARBEPOETIN ALFA IN RABBIT PLASMA BY ELISA Разработка и регистрация лекарственных средств darbepoetin elisa validation pharmacokinetics rabbits plasma |
| title | DETERMINATION OF DARBEPOETIN ALFA IN RABBIT PLASMA BY ELISA |
| title_full | DETERMINATION OF DARBEPOETIN ALFA IN RABBIT PLASMA BY ELISA |
| title_fullStr | DETERMINATION OF DARBEPOETIN ALFA IN RABBIT PLASMA BY ELISA |
| title_full_unstemmed | DETERMINATION OF DARBEPOETIN ALFA IN RABBIT PLASMA BY ELISA |
| title_short | DETERMINATION OF DARBEPOETIN ALFA IN RABBIT PLASMA BY ELISA |
| title_sort | determination of darbepoetin alfa in rabbit plasma by elisa |
| topic | darbepoetin elisa validation pharmacokinetics rabbits plasma |
| url | https://www.pharmjournal.ru/jour/article/view/176 |
| work_keys_str_mv | AT mvkarlina determinationofdarbepoetinalfainrabbitplasmabyelisa AT nmfaustova determinationofdarbepoetinalfainrabbitplasmabyelisa AT onpozharitskaya determinationofdarbepoetinalfainrabbitplasmabyelisa AT vmkosman determinationofdarbepoetinalfainrabbitplasmabyelisa AT vgmakarov determinationofdarbepoetinalfainrabbitplasmabyelisa AT anshikov determinationofdarbepoetinalfainrabbitplasmabyelisa |