Acinetobacter baumannii: Role in Blood Stream Infection in Neonatal Unit, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia
Acinetobacter baumannii (A. baumannii) is Gram-negative coccobacilli that has emerged as a nosocomial pathogen. Several reports in Indonesia showed the continuous presence of A. baumannii. This study aimed to determine the incidence of A. baumannii bacteremia in neonates in the Neonatal Unit Dr. Cip...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2013/180763 |
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| author | Enty Tjoa Lucky Hartati Moehario Andriansjah Rukmana Rinawati Rohsiswatmo |
| author_facet | Enty Tjoa Lucky Hartati Moehario Andriansjah Rukmana Rinawati Rohsiswatmo |
| author_sort | Enty Tjoa |
| collection | DOAJ |
| description | Acinetobacter baumannii (A. baumannii) is Gram-negative coccobacilli that has emerged as a nosocomial pathogen. Several reports in Indonesia showed the continuous presence of A. baumannii. This study aimed to determine the incidence of A. baumannii bacteremia in neonates in the Neonatal Unit Dr. Cipto Mangunkusumo Hospital (RSCM), Jakarta, Indonesia, and assess its role in blood stream infection using antibiogram and genotyping by pulsed-field gel electrophoresis (PFGE). Subjects were neonates with clinical sepsis. Blood specimens from the neonates and samples of suspected environment within the Neonatal Unit were cultivated. Antimicrobial resistance profiles were classified for analysis purpose. A. baumannii isolates were genotyped by PFGE to determine their similarity. A total of 24 A. baumannii were isolated from 80 neonates and the environment during this period of study. Seven isolates from the neonates showed multiple antimicrobial resistance (MDR), and 82% (n=17) of the environment isolates were also MDR. Antibiotype “d” seemed to be predominant (62.5%). PFGE analysis showed a very close genetic relationship between the patients and environment isolates (Dice coefficient 0.8–1.0). We concluded that a mode of transmission of environmental microbes to patients was present in the Neonatal Unit of RSCM and thus needed to be overcome. |
| format | Article |
| id | doaj-art-88f5a47f6e40455db8ff3af18e7602b9 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-88f5a47f6e40455db8ff3af18e7602b92025-08-20T03:38:05ZengWileyInternational Journal of Microbiology1687-918X1687-91982013-01-01201310.1155/2013/180763180763Acinetobacter baumannii: Role in Blood Stream Infection in Neonatal Unit, Dr. Cipto Mangunkusumo Hospital, Jakarta, IndonesiaEnty Tjoa0Lucky Hartati Moehario1Andriansjah Rukmana2Rinawati Rohsiswatmo3Department of Microbiology, Faculty of Medicine, Catholic University of Atma Jaya, Jalan Pluit Raya 2, Jakarta 14440, IndonesiaDepartment of Microbiology, Faculty of Medicine, University of Indonesia, Jalan Pegangsaan Timur 16, Jakarta 10320, IndonesiaDepartment of Microbiology, Faculty of Medicine, University of Indonesia, Jalan Pegangsaan Timur 16, Jakarta 10320, IndonesiaDepartment of Pediatrics, Faculty of Medicine, Neonatal Unit Dr. Cipto Mangunkusumo Hospital, University of Indonesia, Jalan Diponegoro 71, Jakarta 10430, IndonesiaAcinetobacter baumannii (A. baumannii) is Gram-negative coccobacilli that has emerged as a nosocomial pathogen. Several reports in Indonesia showed the continuous presence of A. baumannii. This study aimed to determine the incidence of A. baumannii bacteremia in neonates in the Neonatal Unit Dr. Cipto Mangunkusumo Hospital (RSCM), Jakarta, Indonesia, and assess its role in blood stream infection using antibiogram and genotyping by pulsed-field gel electrophoresis (PFGE). Subjects were neonates with clinical sepsis. Blood specimens from the neonates and samples of suspected environment within the Neonatal Unit were cultivated. Antimicrobial resistance profiles were classified for analysis purpose. A. baumannii isolates were genotyped by PFGE to determine their similarity. A total of 24 A. baumannii were isolated from 80 neonates and the environment during this period of study. Seven isolates from the neonates showed multiple antimicrobial resistance (MDR), and 82% (n=17) of the environment isolates were also MDR. Antibiotype “d” seemed to be predominant (62.5%). PFGE analysis showed a very close genetic relationship between the patients and environment isolates (Dice coefficient 0.8–1.0). We concluded that a mode of transmission of environmental microbes to patients was present in the Neonatal Unit of RSCM and thus needed to be overcome.http://dx.doi.org/10.1155/2013/180763 |
| spellingShingle | Enty Tjoa Lucky Hartati Moehario Andriansjah Rukmana Rinawati Rohsiswatmo Acinetobacter baumannii: Role in Blood Stream Infection in Neonatal Unit, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia International Journal of Microbiology |
| title | Acinetobacter baumannii: Role in Blood Stream Infection in Neonatal Unit, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia |
| title_full | Acinetobacter baumannii: Role in Blood Stream Infection in Neonatal Unit, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia |
| title_fullStr | Acinetobacter baumannii: Role in Blood Stream Infection in Neonatal Unit, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia |
| title_full_unstemmed | Acinetobacter baumannii: Role in Blood Stream Infection in Neonatal Unit, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia |
| title_short | Acinetobacter baumannii: Role in Blood Stream Infection in Neonatal Unit, Dr. Cipto Mangunkusumo Hospital, Jakarta, Indonesia |
| title_sort | acinetobacter baumannii role in blood stream infection in neonatal unit dr cipto mangunkusumo hospital jakarta indonesia |
| url | http://dx.doi.org/10.1155/2013/180763 |
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