Dietary supplementation of male mice with inorganic, organic or nanoparticle selenium preparations: evidence supporting a putative gut-thyroid-male fertility axis
Selenium (Se) is linked to physiological homeostasis. Male mice (n = 8/group) were fed control (AIN93G) or diets enriched in sodium selenite (NaSe, 5.6 ppm), methylselenocysteine (Met, 4.7 ppm), diphenyl diselenide (DPDS, 14.2 ppm), or nanoselenium (NanoSe, 2.7 ppm); dietary Se ascertained by induct...
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Taylor & Francis Group
2025-12-01
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| Series: | Redox Report |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13510002.2025.2495367 |
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| author | A. Mojadadi A. Au T. Ortiz Cerda J.-Y. Shao T. O’Neil K. Bell-Anderson J. W. Andersen J. Webb W. Salah G. Ahmad H. H. Harris P. K. Witting |
| author_facet | A. Mojadadi A. Au T. Ortiz Cerda J.-Y. Shao T. O’Neil K. Bell-Anderson J. W. Andersen J. Webb W. Salah G. Ahmad H. H. Harris P. K. Witting |
| author_sort | A. Mojadadi |
| collection | DOAJ |
| description | Selenium (Se) is linked to physiological homeostasis. Male mice (n = 8/group) were fed control (AIN93G) or diets enriched in sodium selenite (NaSe, 5.6 ppm), methylselenocysteine (Met, 4.7 ppm), diphenyl diselenide (DPDS, 14.2 ppm), or nanoselenium (NanoSe, 2.7 ppm); dietary Se ascertained by inductively-coupled plasma mass spectrometry. At 4 weeks testes, sperm, thyroids, blood and stool were collected to assess histoarchitecture, circulating hormones (thyroxine, T4; triiodothyronine, T3; thyroid stimulating hormone, TSH) and gut microbiome (16S rRNAV3-V4 amplicon sequencing). Supplemented NaSe, Met, and NanoSe increased plasma testosterone and testis glutathione peroxidases (GPx-1/4) while testicular superoxide dismutase and catalase increased slightly in the NanoSe group indicating a selective antioxidant response. Overall, NanoSe and NaSe enhanced male reproductive factors. All thyroids isolated from Se-supplemented mice contained marginal vacuoles and a lower follicle area vs control. Nano-Se enhanced thyroidiodothyronine deiodinase-1 (DIO1) expression however, thyroid GPx-1/4 remained unchanged. Supplemented NaSe and DPDSl increased plasma T3/T4 ratio, while plasma TSH was unchanged. Microbiome analyses showed that NanoSe was most efficacious in altering composition (judged by α-diversity, Shannon index and taxon richness) while the NaSe diet showed the greatest overall change in α-diversity. Dietary Se supplementation, particularly encapsulated NanoSe, may improve male fertility factors by enhancing the gut-thyroid-fertility axis. |
| format | Article |
| id | doaj-art-88f53468ecd846e8b1c7f1515d91ac3d |
| institution | DOAJ |
| issn | 1351-0002 1743-2928 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Redox Report |
| spelling | doaj-art-88f53468ecd846e8b1c7f1515d91ac3d2025-08-20T03:14:19ZengTaylor & Francis GroupRedox Report1351-00021743-29282025-12-0130110.1080/13510002.2025.2495367Dietary supplementation of male mice with inorganic, organic or nanoparticle selenium preparations: evidence supporting a putative gut-thyroid-male fertility axisA. Mojadadi0A. Au1T. Ortiz Cerda2J.-Y. Shao3T. O’Neil4K. Bell-Anderson5J. W. Andersen6J. Webb7W. Salah8G. Ahmad9H. H. Harris10P. K. Witting11Redox Biology Group, Charles Perkins Centre, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, AustraliaRedox Biology Group, Charles Perkins Centre, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, AustraliaDepartamento de Citología e Histología Normal y Patológica, Facultad de medicina, Universidad de Sevilla, Seville, SpainRedox Biology Group, Charles Perkins Centre, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, AustraliaRedox Biology Group, Charles Perkins Centre, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, AustraliaDiscipline of Nutrition, School of Life and Environmental Sciences, The University of Sydney, Sydney, AustraliaSchool of Chemistry and Physics, The University of Adelaide, Adelaide, AustraliaSchool of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Sydney, AustraliaRedox Biology Group, Charles Perkins Centre, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, AustraliaRedox Biology Group, Charles Perkins Centre, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, AustraliaDiscipline of Nutrition, School of Life and Environmental Sciences, The University of Sydney, Sydney, AustraliaRedox Biology Group, Charles Perkins Centre, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, AustraliaSelenium (Se) is linked to physiological homeostasis. Male mice (n = 8/group) were fed control (AIN93G) or diets enriched in sodium selenite (NaSe, 5.6 ppm), methylselenocysteine (Met, 4.7 ppm), diphenyl diselenide (DPDS, 14.2 ppm), or nanoselenium (NanoSe, 2.7 ppm); dietary Se ascertained by inductively-coupled plasma mass spectrometry. At 4 weeks testes, sperm, thyroids, blood and stool were collected to assess histoarchitecture, circulating hormones (thyroxine, T4; triiodothyronine, T3; thyroid stimulating hormone, TSH) and gut microbiome (16S rRNAV3-V4 amplicon sequencing). Supplemented NaSe, Met, and NanoSe increased plasma testosterone and testis glutathione peroxidases (GPx-1/4) while testicular superoxide dismutase and catalase increased slightly in the NanoSe group indicating a selective antioxidant response. Overall, NanoSe and NaSe enhanced male reproductive factors. All thyroids isolated from Se-supplemented mice contained marginal vacuoles and a lower follicle area vs control. Nano-Se enhanced thyroidiodothyronine deiodinase-1 (DIO1) expression however, thyroid GPx-1/4 remained unchanged. Supplemented NaSe and DPDSl increased plasma T3/T4 ratio, while plasma TSH was unchanged. Microbiome analyses showed that NanoSe was most efficacious in altering composition (judged by α-diversity, Shannon index and taxon richness) while the NaSe diet showed the greatest overall change in α-diversity. Dietary Se supplementation, particularly encapsulated NanoSe, may improve male fertility factors by enhancing the gut-thyroid-fertility axis.https://www.tandfonline.com/doi/10.1080/13510002.2025.2495367Dietary seleniumnanoseleniummale fertilitytestosteronethyroidmicrobiota |
| spellingShingle | A. Mojadadi A. Au T. Ortiz Cerda J.-Y. Shao T. O’Neil K. Bell-Anderson J. W. Andersen J. Webb W. Salah G. Ahmad H. H. Harris P. K. Witting Dietary supplementation of male mice with inorganic, organic or nanoparticle selenium preparations: evidence supporting a putative gut-thyroid-male fertility axis Redox Report Dietary selenium nanoselenium male fertility testosterone thyroid microbiota |
| title | Dietary supplementation of male mice with inorganic, organic or nanoparticle selenium preparations: evidence supporting a putative gut-thyroid-male fertility axis |
| title_full | Dietary supplementation of male mice with inorganic, organic or nanoparticle selenium preparations: evidence supporting a putative gut-thyroid-male fertility axis |
| title_fullStr | Dietary supplementation of male mice with inorganic, organic or nanoparticle selenium preparations: evidence supporting a putative gut-thyroid-male fertility axis |
| title_full_unstemmed | Dietary supplementation of male mice with inorganic, organic or nanoparticle selenium preparations: evidence supporting a putative gut-thyroid-male fertility axis |
| title_short | Dietary supplementation of male mice with inorganic, organic or nanoparticle selenium preparations: evidence supporting a putative gut-thyroid-male fertility axis |
| title_sort | dietary supplementation of male mice with inorganic organic or nanoparticle selenium preparations evidence supporting a putative gut thyroid male fertility axis |
| topic | Dietary selenium nanoselenium male fertility testosterone thyroid microbiota |
| url | https://www.tandfonline.com/doi/10.1080/13510002.2025.2495367 |
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