Dynamics of DNA methylation during osteogenic differentiation of porcine synovial membrane mesenchymal stem cells from two metabolically distinct breeds
Mesenchymal stem cells (MSCs), with the ability to differentiate into osteoblasts, adipocytes, or chondrocytes, show evidence that the donor cell’s metabolic type influences the osteogenic process. Limited knowledge exists on DNA methylation changes during osteogenic differentiation and the impact o...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Epigenetics |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/15592294.2024.2375011 |
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| author | Shuaichen Li Puntita Siengdee Frieder Hadlich Nares Trakooljul Michael Oster Henry Reyer Klaus Wimmers Siriluck Ponsuksili |
| author_facet | Shuaichen Li Puntita Siengdee Frieder Hadlich Nares Trakooljul Michael Oster Henry Reyer Klaus Wimmers Siriluck Ponsuksili |
| author_sort | Shuaichen Li |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSCs), with the ability to differentiate into osteoblasts, adipocytes, or chondrocytes, show evidence that the donor cell’s metabolic type influences the osteogenic process. Limited knowledge exists on DNA methylation changes during osteogenic differentiation and the impact of diverse donor genetic backgrounds on MSC differentiation. In this study, synovial membrane mesenchymal stem cells (SMSCs) from two pig breeds (Angeln Saddleback, AS; German Landrace, DL) with distinct metabolic phenotypes were isolated, and the methylation pattern of SMSCs during osteogenic induction was investigated. Results showed that most differentially methylated regions (DMRs) were hypomethylated in osteogenic-induced SMSC group. These DMRs were enriched with genes of different osteogenic signalling pathways at different time points including Wnt, ECM, TGFB and BMP signalling pathways. AS pigs consistently exhibited a higher number of hypermethylated DMRs than DL pigs, particularly during the peak of osteogenesis (day 21). Predicting transcription factor motifs in regions of DMRs linked to osteogenic processes and donor breeds revealed influential motifs, including KLF1, NFATC3, ZNF148, ASCL1, FOXI1, and KLF5. These findings contribute to understanding the pattern of methylation changes promoting osteogenic differentiation, emphasizing the substantial role of donor the metabolic type and epigenetic memory of different donors on SMSC differentiation. |
| format | Article |
| id | doaj-art-88e9d7aa83b84518abb8fcba29664ea0 |
| institution | OA Journals |
| issn | 1559-2294 1559-2308 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Epigenetics |
| spelling | doaj-art-88e9d7aa83b84518abb8fcba29664ea02025-08-20T02:20:49ZengTaylor & Francis GroupEpigenetics1559-22941559-23082024-12-0119110.1080/15592294.2024.2375011Dynamics of DNA methylation during osteogenic differentiation of porcine synovial membrane mesenchymal stem cells from two metabolically distinct breedsShuaichen Li0Puntita Siengdee1Frieder Hadlich2Nares Trakooljul3Michael Oster4Henry Reyer5Klaus Wimmers6Siriluck Ponsuksili7Institute of Genome Biology, Research Institute for Farm Animal Biology (FBN), Dummerstorf, GermanyInstitute of Genome Biology, Research Institute for Farm Animal Biology (FBN), Dummerstorf, GermanyInstitute of Genome Biology, Research Institute for Farm Animal Biology (FBN), Dummerstorf, GermanyInstitute of Genome Biology, Research Institute for Farm Animal Biology (FBN), Dummerstorf, GermanyInstitute of Genome Biology, Research Institute for Farm Animal Biology (FBN), Dummerstorf, GermanyInstitute of Genome Biology, Research Institute for Farm Animal Biology (FBN), Dummerstorf, GermanyInstitute of Genome Biology, Research Institute for Farm Animal Biology (FBN), Dummerstorf, GermanyInstitute of Genome Biology, Research Institute for Farm Animal Biology (FBN), Dummerstorf, GermanyMesenchymal stem cells (MSCs), with the ability to differentiate into osteoblasts, adipocytes, or chondrocytes, show evidence that the donor cell’s metabolic type influences the osteogenic process. Limited knowledge exists on DNA methylation changes during osteogenic differentiation and the impact of diverse donor genetic backgrounds on MSC differentiation. In this study, synovial membrane mesenchymal stem cells (SMSCs) from two pig breeds (Angeln Saddleback, AS; German Landrace, DL) with distinct metabolic phenotypes were isolated, and the methylation pattern of SMSCs during osteogenic induction was investigated. Results showed that most differentially methylated regions (DMRs) were hypomethylated in osteogenic-induced SMSC group. These DMRs were enriched with genes of different osteogenic signalling pathways at different time points including Wnt, ECM, TGFB and BMP signalling pathways. AS pigs consistently exhibited a higher number of hypermethylated DMRs than DL pigs, particularly during the peak of osteogenesis (day 21). Predicting transcription factor motifs in regions of DMRs linked to osteogenic processes and donor breeds revealed influential motifs, including KLF1, NFATC3, ZNF148, ASCL1, FOXI1, and KLF5. These findings contribute to understanding the pattern of methylation changes promoting osteogenic differentiation, emphasizing the substantial role of donor the metabolic type and epigenetic memory of different donors on SMSC differentiation.https://www.tandfonline.com/doi/10.1080/15592294.2024.2375011DNA methylationMesenchymal stem cellsOsteogenic differentiationPig breedsEpigenetic pattern |
| spellingShingle | Shuaichen Li Puntita Siengdee Frieder Hadlich Nares Trakooljul Michael Oster Henry Reyer Klaus Wimmers Siriluck Ponsuksili Dynamics of DNA methylation during osteogenic differentiation of porcine synovial membrane mesenchymal stem cells from two metabolically distinct breeds Epigenetics DNA methylation Mesenchymal stem cells Osteogenic differentiation Pig breeds Epigenetic pattern |
| title | Dynamics of DNA methylation during osteogenic differentiation of porcine synovial membrane mesenchymal stem cells from two metabolically distinct breeds |
| title_full | Dynamics of DNA methylation during osteogenic differentiation of porcine synovial membrane mesenchymal stem cells from two metabolically distinct breeds |
| title_fullStr | Dynamics of DNA methylation during osteogenic differentiation of porcine synovial membrane mesenchymal stem cells from two metabolically distinct breeds |
| title_full_unstemmed | Dynamics of DNA methylation during osteogenic differentiation of porcine synovial membrane mesenchymal stem cells from two metabolically distinct breeds |
| title_short | Dynamics of DNA methylation during osteogenic differentiation of porcine synovial membrane mesenchymal stem cells from two metabolically distinct breeds |
| title_sort | dynamics of dna methylation during osteogenic differentiation of porcine synovial membrane mesenchymal stem cells from two metabolically distinct breeds |
| topic | DNA methylation Mesenchymal stem cells Osteogenic differentiation Pig breeds Epigenetic pattern |
| url | https://www.tandfonline.com/doi/10.1080/15592294.2024.2375011 |
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