Synergistic effect of Maquiberry cystatin, sodium fluoride and stannous chloride for the prevention of initial dental erosion in vitro
Abstract Objective This study analyzed the synergistic effect between a recombinant Maquiberry (MaquiCPI-3) protein, sodium fluoride (NaF), and stannous chloride (SnCl2) against initial dental erosion in vitro. Methodology A total of 98 bovine enamel samples were prepared and allocated to seven...
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University of São Paulo
2025-06-01
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| Series: | Journal of Applied Oral Science |
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| author | Vinícius Taioqui PELÁ Thelma Lopes SILVA Even Akemi TAIRA Geórgia Almeida SANT’ANA Letícia Oba SAKAE Flávia Mauad LEVY Taís Scaramucci FORLIN Eduardo Pereira de SOUZA Talita Mendes Oliveira VENTURA Thiago Saads CARVALHO Adrian LUSSI Marília Afonso Rabelo BUZALAF |
| author_facet | Vinícius Taioqui PELÁ Thelma Lopes SILVA Even Akemi TAIRA Geórgia Almeida SANT’ANA Letícia Oba SAKAE Flávia Mauad LEVY Taís Scaramucci FORLIN Eduardo Pereira de SOUZA Talita Mendes Oliveira VENTURA Thiago Saads CARVALHO Adrian LUSSI Marília Afonso Rabelo BUZALAF |
| author_sort | Vinícius Taioqui PELÁ |
| collection | DOAJ |
| description | Abstract Objective This study analyzed the synergistic effect between a recombinant Maquiberry (MaquiCPI-3) protein, sodium fluoride (NaF), and stannous chloride (SnCl2) against initial dental erosion in vitro. Methodology A total of 98 bovine enamel samples were prepared and allocated to seven treatment groups (n=14/group) as follows: deionized water (Water); commercial solution, 800 ppm Sn+2, 500 ppm F-, ElmexTM, Erosion, GABA, Therwil, BL, CH (Elmex); 500 ppm of fluoride (F-) from NaF (NaF); 500 ppm of F- from NaF and 800 ppm of tin (Sn+2) from SnCl2 (NaF+SnCl); 0.5 mg/mL MaquiCPI-3 (MaquiCPI-3); combination of MaquiCPI-3 and NaF (Maqui+NaF) and; combination of MaquiCPI-3, NaF and SnCl (Maqui+NaF+SnCl). Samples were treated with the respective solutions (250 μl, 2 min, 37°C, 250 rpm). After forming the acquired enamel pellicle (AEP) by adding human saliva from nine volunteers (250 μl, 1 h, 37°C, 250 rpm), the samples underwent acid challenge (1 mL, 1% citric acid, pH 3.6, 1 min, 25°C, 250 rpm). All procedures were performed in triplicate. Surface microhardness change percentage (%SMC) and relative surface reflection intensity (%SRI) were measured and analyzed by One-way ANOVA/Tukey’s tests (p<0.05). Results The Elmex, NaF, NaF+SnCl, MaquiCPI-3, Maqui+NaF, and Maqui+NaF+SnCl groups showed significantly lower %SMC compared with Water. The NaF, Sn+2, NaF+SnCl, MaquiCPI-3, Maqui+NaF and Maqui+NaF+SnCl groups exhibited significantly greater protection compared with Elmex. Maqui+NaF+SnCl revealed better enamel protection (significant) when compared with the MaquiCPI-3 and Maqui+NaF groups. Elmex, NaF+SnCl, MaquiCPI-3, Maqui+NaF, and Maqui+NaF+SnCl had a significantly higher %SRI compared to the Water and NaF groups, which did not differ between each other. Conclusion All treatment solutions provided protection against initial dental erosion in vitro. Formulations containing Maqui+NaF+SnCl2 offered superior enamel protection compared with MaquiCPI-3 alone. |
| format | Article |
| id | doaj-art-88e57e7deaac435da0c3cc3cd6f04296 |
| institution | DOAJ |
| issn | 1678-7765 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | University of São Paulo |
| record_format | Article |
| series | Journal of Applied Oral Science |
| spelling | doaj-art-88e57e7deaac435da0c3cc3cd6f042962025-08-20T03:11:02ZengUniversity of São PauloJournal of Applied Oral Science1678-77652025-06-013310.1590/1678-7757-2024-0479Synergistic effect of Maquiberry cystatin, sodium fluoride and stannous chloride for the prevention of initial dental erosion in vitroVinícius Taioqui PELÁhttps://orcid.org/0000-0001-7933-4422Thelma Lopes SILVAhttps://orcid.org/0009-0006-5622-2966Even Akemi TAIRAhttps://orcid.org/0000-0003-0853-1335Geórgia Almeida SANT’ANAhttps://orcid.org/0009-0009-0688-9094Letícia Oba SAKAEhttps://orcid.org/0000-0003-3885-4450Flávia Mauad LEVYhttps://orcid.org/0000-0002-4912-5272Taís Scaramucci FORLINhttps://orcid.org/0000-0002-4445-6697Eduardo Pereira de SOUZAhttps://orcid.org/0000-0002-2013-0115Talita Mendes Oliveira VENTURAhttps://orcid.org/0000-0003-2101-1350Thiago Saads CARVALHOhttps://orcid.org/0000-0002-2435-1169Adrian LUSSIhttps://orcid.org/0000-0002-0426-3364Marília Afonso Rabelo BUZALAFhttps://orcid.org/0000-0002-5985-3951Abstract Objective This study analyzed the synergistic effect between a recombinant Maquiberry (MaquiCPI-3) protein, sodium fluoride (NaF), and stannous chloride (SnCl2) against initial dental erosion in vitro. Methodology A total of 98 bovine enamel samples were prepared and allocated to seven treatment groups (n=14/group) as follows: deionized water (Water); commercial solution, 800 ppm Sn+2, 500 ppm F-, ElmexTM, Erosion, GABA, Therwil, BL, CH (Elmex); 500 ppm of fluoride (F-) from NaF (NaF); 500 ppm of F- from NaF and 800 ppm of tin (Sn+2) from SnCl2 (NaF+SnCl); 0.5 mg/mL MaquiCPI-3 (MaquiCPI-3); combination of MaquiCPI-3 and NaF (Maqui+NaF) and; combination of MaquiCPI-3, NaF and SnCl (Maqui+NaF+SnCl). Samples were treated with the respective solutions (250 μl, 2 min, 37°C, 250 rpm). After forming the acquired enamel pellicle (AEP) by adding human saliva from nine volunteers (250 μl, 1 h, 37°C, 250 rpm), the samples underwent acid challenge (1 mL, 1% citric acid, pH 3.6, 1 min, 25°C, 250 rpm). All procedures were performed in triplicate. Surface microhardness change percentage (%SMC) and relative surface reflection intensity (%SRI) were measured and analyzed by One-way ANOVA/Tukey’s tests (p<0.05). Results The Elmex, NaF, NaF+SnCl, MaquiCPI-3, Maqui+NaF, and Maqui+NaF+SnCl groups showed significantly lower %SMC compared with Water. The NaF, Sn+2, NaF+SnCl, MaquiCPI-3, Maqui+NaF and Maqui+NaF+SnCl groups exhibited significantly greater protection compared with Elmex. Maqui+NaF+SnCl revealed better enamel protection (significant) when compared with the MaquiCPI-3 and Maqui+NaF groups. Elmex, NaF+SnCl, MaquiCPI-3, Maqui+NaF, and Maqui+NaF+SnCl had a significantly higher %SRI compared to the Water and NaF groups, which did not differ between each other. Conclusion All treatment solutions provided protection against initial dental erosion in vitro. Formulations containing Maqui+NaF+SnCl2 offered superior enamel protection compared with MaquiCPI-3 alone.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572025000100424&lng=en&tlng=enDemineralizationEnamelSaliva |
| spellingShingle | Vinícius Taioqui PELÁ Thelma Lopes SILVA Even Akemi TAIRA Geórgia Almeida SANT’ANA Letícia Oba SAKAE Flávia Mauad LEVY Taís Scaramucci FORLIN Eduardo Pereira de SOUZA Talita Mendes Oliveira VENTURA Thiago Saads CARVALHO Adrian LUSSI Marília Afonso Rabelo BUZALAF Synergistic effect of Maquiberry cystatin, sodium fluoride and stannous chloride for the prevention of initial dental erosion in vitro Journal of Applied Oral Science Demineralization Enamel Saliva |
| title | Synergistic effect of Maquiberry cystatin, sodium fluoride and stannous chloride for the prevention of initial dental erosion in vitro |
| title_full | Synergistic effect of Maquiberry cystatin, sodium fluoride and stannous chloride for the prevention of initial dental erosion in vitro |
| title_fullStr | Synergistic effect of Maquiberry cystatin, sodium fluoride and stannous chloride for the prevention of initial dental erosion in vitro |
| title_full_unstemmed | Synergistic effect of Maquiberry cystatin, sodium fluoride and stannous chloride for the prevention of initial dental erosion in vitro |
| title_short | Synergistic effect of Maquiberry cystatin, sodium fluoride and stannous chloride for the prevention of initial dental erosion in vitro |
| title_sort | synergistic effect of maquiberry cystatin sodium fluoride and stannous chloride for the prevention of initial dental erosion in vitro |
| topic | Demineralization Enamel Saliva |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572025000100424&lng=en&tlng=en |
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